DNA-Based Immunization Breaks Tolerance in a Hepatitis C Virus Transgenic Mouse Model

2006 ◽  
Vol 2 (2) ◽  
pp. 78-83 ◽  
Author(s):  
Jens Encke ◽  
Michael Geissler ◽  
Wolfgang Stremmel ◽  
Jack R. Wands
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Mouse Model ◽  
Transgenic Mouse ◽  
Transgenic Mouse Model

scholarly journals Alcohol Induced Hepatic Degeneration in a Hepatitis C Virus Core Protein Transgenic Mouse Model

2014 ◽  
Vol 15 (3) ◽  
pp. 4126-4141 ◽  
Author(s):  
Dong-Hyung Noh ◽  
Eun-Joo Lee ◽  
Ah-Young Kim ◽  
Eun-Mi Lee ◽  
Chang-Woo Min ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Mouse Model ◽  
Transgenic Mouse ◽  
Core Protein ◽  
Transgenic Mouse Model ◽  
Virus Core

scholarly journals Helicobacter pyloriinfection does not promote hepatocellular cancer in a transgenic mouse model of hepatitis C virus pathogenesis

Gut Microbes ◽  
2013 ◽  
Vol 4 (6) ◽  
pp. 577-590 ◽  
Author(s):  
Alexis García ◽  
Yan Feng ◽  
Nicola MA Parry ◽  
Amanda McCabe ◽  
Melissa W Mobley ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Mouse Model ◽  
Transgenic Mouse ◽  
Hepatocellular Cancer ◽  
Transgenic Mouse Model

scholarly journals Characterization of liver histopathology in a transgenic mouse model expressing genotype 1a hepatitis C virus core and envelope proteins 1 and 2

2005 ◽  
Vol 86 (8) ◽  
pp. 2185-2196 ◽  
Author(s):  
Turaya Naas ◽  
Masoud Ghorbani ◽  
Ikuri Alvarez-Maya ◽  
Michael Lapner ◽  
Rashmi Kothary ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Mouse Model ◽  
Protein Expression ◽  
Transgenic Mouse ◽  
Transgenic Mouse Model ◽  
Structural Proteins ◽  
Hcv Rna ◽  
Hcv Structural Proteins

Hepatitis C virus (HCV) is a major cause of chronic hepatitis and hepatocellular carcinoma worldwide. The purpose of this study was to determine how the HCV structural proteins affect the dynamic structural and functional properties of hepatocytes and measure the extra-hepatic manifestations induced by these viral proteins. A transgenic mouse model was established by expressing core, E1 and E2 proteins downstream of a CMV promoter. HCV RNA was detected using RT-PCR in transgenic mouse model tissues, such as liver, kidney, spleen and heart. Expression of the transgene was analysed by real-time PCR to quantify viral RNA in different tissues at different ages. Immunofluorescence analysis revealed the expression of core, E1 and E2 proteins predominantly in hepatocytes. Lower levels of protein expression were detected in spleen and kidneys. HCV RNA and viral protein expression increased in the liver with age. Histological analysis of liver cells demonstrated steatosis in transgenic mice older than 3 months, which was more progressed with age. Electron microscopy analysis revealed alterations in nuclei, mitochondria and endoplasmic reticulum. HCV structural proteins induce a severe hepatopathy in the transgenic mouse model. These mice became more prone to liver and lymphoid tumour development and hepatocellular carcinoma. In this model, the extra-hepatic effects of HCV, which included swelling of renal tubular cells, were mild. It is likely that the HCV structural proteins mediate some of the histological alterations in hepatocytes by interfering with lipid transport and liver metabolism.

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