scholarly journals Human islet mass, morphology, and survival after cryopreservation using the Edmonton protocol

Islets ◽  
2013 ◽  
Vol 5 (5) ◽  
pp. 188-195 ◽  
Author(s):  
Priya M Miranda ◽  
Viswanathan Mohan ◽  
Sekhar Ganthimathy ◽  
Ranjit M Anjana ◽  
S Gunasekaran ◽  
...  
Keyword(s):  
Human Islet ◽  
Islet Mass ◽  
Edmonton Protocol

scholarly journals A Smartphone-Fluidic Digital Imaging Analysis System for Pancreatic Islet Mass Quantification

2021 ◽  
Vol 9 ◽  
Author(s):  
Xiaoyu Yu ◽  
Pu Zhang ◽  
Yi He ◽  
Emily Lin ◽  
Huiwang Ai ◽  
...  
Keyword(s):  
Digital Imaging ◽  
Video Processing ◽  
Microfluidic Channel ◽  
Middle Layer ◽  
Average Diameter ◽  
Human Islet ◽  
Imaging Analysis ◽  
Islet Mass ◽  
Manual Counting ◽  
Analysis System

Islet beta-cell viability, function, and mass are three decisive attributes that determine the efficacy of human islet transplantation for type 1 diabetes mellitus (T1DM) patients. Islet mass is commonly assessed manually, which often leads to error and bias. Digital imaging analysis (DIA) system has shown its potential as an alternative, but it has some associated limitations. In this study, a Smartphone-Fluidic Digital Imaging Analysis (SFDIA) System, which incorporates microfluidic techniques and Python-based video processing software, was developed for islet mass assessment. We quantified islets by tracking multiple moving islets in a microfluidic channel using the SFDIA system, and we achieved a relatively consistent result. The counts from the SFDIA and manual counting showed an average difference of 2.91 ± 1.50%. Furthermore, our software can analyze and extract key human islet mass parameters, including quantity, size, volume, IEq, morphology, and purity, which are not fully obtainable from traditional manual counting methods. Using SFDIA on a representative islet sample, we measured an average diameter of 99.88 ± 53.91 µm, an average circularity of 0.591 ± 0.133, and an average solidity of 0.853 ± 0.107. Via analysis of dithizone-stained islets using SFDIA, we found that a higher islet tissue percentage is associated with top-layer islets as opposed to middle-layer islets (0.735 ± 0.213 and 0.576 ± 0.223, respectively). Our results indicate that the SFDIA system can potentially be used as a multi-parameter islet mass assay that is superior in accuracy and consistency, when compared to conventional manual techniques.

scholarly journals Extensive Loss of Islet Mass beyond the First Day after Intraportal Human Islet Transplantation in a Mouse Model

2016 ◽  
Vol 25 (3) ◽  
pp. 481-489 ◽  
Author(s):  
Hanna Liljebäck ◽  
Liza Grapensparr ◽  
Johan Olerud ◽  
Per-Ola Carlsson
Keyword(s):  
Mouse Model ◽  
Islet Transplantation ◽  
Human Islet ◽  
Islet Mass ◽  
Human Islet Transplantation

International Trial of the Edmonton Protocol for Islet Transplantation

2007 ◽  
Vol 2007 ◽  
pp. 6-9
Author(s):  
L. Kennedy ◽  
J.L. Leahy
Keyword(s):  
Islet Transplantation ◽  
International Trial ◽  
Edmonton Protocol

2056-P: In Vivo Human Islet Compensation and Dysfunction to Diet-Induced Obesity: A Phase 0 Preclinical Model

Diabetes ◽  
2020 ◽  
Vol 69 (Supplement 1) ◽  
pp. 2056-P
Author(s):  
JULIE A. KERR-CONTE ◽  
JULIEN THEVENET ◽  
GIANNI PASQUETTI ◽  
PAULINE PETIT ◽  
CLARA CLABAUT ◽  
...  
Keyword(s):  
Human Islet ◽  
Preclinical Model ◽  
Diet Induced Obesity ◽  
Phase 0

Human islet amyloid polypeptide accumulates at similar sites in islets of transgenic mice and humans

Diabetes ◽  
1994 ◽  
Vol 43 (5) ◽  
pp. 640-644 ◽  
Author(s):  
E. J. de Koning ◽  
J. W. Hoppener ◽  
J. S. Verbeek ◽  
C. Oosterwijk ◽  
K. L. van Hulst ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Islet Amyloid Polypeptide ◽  
Human Islet ◽  
Islet Amyloid ◽  
Human Islet Amyloid Polypeptide

Insulin secretory function in relation to transplanted islet mass in STZ-induced diabetic rats

Diabetes ◽  
1993 ◽  
Vol 42 (1) ◽  
pp. 98-105 ◽  
Author(s):  
B. W. Tobin ◽  
J. T. Lewis ◽  
D. Z. Chen ◽  
D. T. Finegood
Keyword(s):  
Diabetic Rats ◽  
Secretory Function ◽  
Islet Mass

Culture of adult human islet preparations with hepatocyte growth factor and 804G matrix is mitogenic for duct cells but not for beta-cells

Diabetes ◽  
1998 ◽  
Vol 47 (1) ◽  
pp. 134-137 ◽  
Author(s):  
V. H. Lefebvre ◽  
T. Otonkoski ◽  
J. Ustinov ◽  
M. A. Huotari ◽  
D. G. Pipeleers ◽  
...  
Keyword(s):  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Beta Cells ◽  
Human Islet ◽  
Adult Human ◽  
Duct Cells ◽  
Hepatocyte Growth

Human Islet Transplantation: Current Status and Future Direction

2013 ◽  
Vol 5 (3) ◽  
pp. 156-161
Author(s):  
James McGarrigle ◽  
Jose Oberholzer
Keyword(s):  
Islet Transplantation ◽  
Human Islet ◽  
Current Status ◽  
Human Islet Transplantation ◽  
Future Direction

Editorial (Hot Topic: Current Applications of Micro and Nano Technology in Human Islet Transplantation)

2013 ◽  
Vol 5 (3) ◽  
pp. 155-155
Author(s):  
James McGarrigle ◽  
Jose Oberholzer
Keyword(s):  
Islet Transplantation ◽  
Human Islet ◽  
Nano Technology ◽  
Human Islet Transplantation
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