scholarly journals A Smartphone-Fluidic Digital Imaging Analysis System for Pancreatic Islet Mass Quantification

2021 ◽  
Vol 9 ◽  
Author(s):  
Xiaoyu Yu ◽  
Pu Zhang ◽  
Yi He ◽  
Emily Lin ◽  
Huiwang Ai ◽  
...  
Keyword(s):  
Digital Imaging ◽  
Video Processing ◽  
Microfluidic Channel ◽  
Middle Layer ◽  
Average Diameter ◽  
Human Islet ◽  
Imaging Analysis ◽  
Islet Mass ◽  
Manual Counting ◽  
Analysis System

Islet beta-cell viability, function, and mass are three decisive attributes that determine the efficacy of human islet transplantation for type 1 diabetes mellitus (T1DM) patients. Islet mass is commonly assessed manually, which often leads to error and bias. Digital imaging analysis (DIA) system has shown its potential as an alternative, but it has some associated limitations. In this study, a Smartphone-Fluidic Digital Imaging Analysis (SFDIA) System, which incorporates microfluidic techniques and Python-based video processing software, was developed for islet mass assessment. We quantified islets by tracking multiple moving islets in a microfluidic channel using the SFDIA system, and we achieved a relatively consistent result. The counts from the SFDIA and manual counting showed an average difference of 2.91 ± 1.50%. Furthermore, our software can analyze and extract key human islet mass parameters, including quantity, size, volume, IEq, morphology, and purity, which are not fully obtainable from traditional manual counting methods. Using SFDIA on a representative islet sample, we measured an average diameter of 99.88 ± 53.91 µm, an average circularity of 0.591 ± 0.133, and an average solidity of 0.853 ± 0.107. Via analysis of dithizone-stained islets using SFDIA, we found that a higher islet tissue percentage is associated with top-layer islets as opposed to middle-layer islets (0.735 ± 0.213 and 0.576 ± 0.223, respectively). Our results indicate that the SFDIA system can potentially be used as a multi-parameter islet mass assay that is superior in accuracy and consistency, when compared to conventional manual techniques.

scholarly journals Objective assessment of vitiligo with a computerised digital imaging analysis system

2014 ◽  
Vol 56 (4) ◽  
pp. 285-289 ◽  
Author(s):  
Norashikin Shamsudin ◽  
Suraiya H Hussein ◽  
Hermawan Nugroho ◽  
Mohd Hani Ahmad Fadzil
Keyword(s):  
Digital Imaging ◽  
Objective Assessment ◽  
Imaging Analysis ◽  
Analysis System

scholarly journals Digital Imaging Analysis with Fractal Dimension in Oral Leukoplakia

2017 ◽  
Vol 2 (1) ◽  
pp. 1-5
Author(s):  
CH Reddy ◽  
M Anitha ◽  
A Feroz ◽  
L Chandrashekar ◽  
R Sudarshan ◽  
...  
Keyword(s):  
Fractal Dimension ◽  
Digital Imaging ◽  
Oral Leukoplakia ◽  
Imaging Analysis

Detection of Directivity of Fluorescence From a Mixed Specimen Which Consists of Micro Particles Attached Different Fluorescent Substances Using a Light Waveguide Incorporated Optical TAS

2018 ◽  
Author(s):  
Toshifumi Ohkubo ◽  
Nobuyuki Terada ◽  
Yoshikazu Yoshida
Keyword(s):  
Microfluidic Channel ◽  
Laser Source ◽  
Microfluidic Channels ◽  
Fluorescent Substance ◽  
Total Analysis System ◽  
Micro Particles ◽  
Total Analysis ◽  
Light Waveguide ◽  
Analysis System ◽  
Near Future

A resin-based optical total analysis system (O-TAS) which consists both of microfluidic channels and light waveguides [1] is thought to be one of the most promising components in developing a “ubiquitous human healthcare system” in the near future. Along with this technology trend, we have already developed a transparent epoxy-resin-based optical TAS chip which has a specially prepared light waveguide structure of radially arranged configuration at an intersection portion with a microfluidic channel, in order to detect directivity of fluorescence from fluorescent substance attached micro particles [2],[3]. Schematic diagram of the optical TAS is shown in Figure 1. In the latest research, utilizing an AC modulated laser source and time-series averaging function on detected signal waveforms, we could have successfully obtained directivities of fluorescence from 5-μm-diameter particles with higher signal to noise (S/N) ratio [3].

scholarly journals Genome-wide association for grain morphology in synthetic hexaploid wheats using digital imaging analysis

2014 ◽  
Vol 14 (1) ◽  
pp. 128 ◽  
Author(s):  
Awais Rasheed ◽  
Xianchun Xia ◽  
Francis Ogbonnaya ◽  
Tariq Mahmood ◽  
Zongwen Zhang ◽  
...  
Keyword(s):  
Digital Imaging ◽  
Grain Morphology ◽  
Genome Wide Association ◽  
Imaging Analysis ◽  
Genome Wide ◽  
Synthetic Hexaploid Wheats ◽  
Synthetic Hexaploid

High-speed digital imaging laryngoscopy of the neoglottis following supracricoid laryngectomy with cricohyoidoepiglottopexy

2010 ◽  
Vol 124 (11) ◽  
pp. 1234-1238 ◽  
Author(s):  
S Hayashi ◽  
H Hirose ◽  
N Tayama ◽  
H Imagawa ◽  
M Nakayama ◽  
...  
Keyword(s):  
Fundamental Frequency ◽  
Sound Source ◽  
Digital Imaging ◽  
High Speed ◽  
Imaging System ◽  
Vocal Performance ◽  
Inverse Filtering ◽  
Imaging Analysis ◽  
Supracricoid Laryngectomy ◽  
Digital Imaging System

AbstractObjectives:This study aimed to analyse vocal performance and to investigate the nature of the neoglottal sound source in patients who had undergone supracricoid laryngectomy with cricohyoidoepiglottopexy, using a high-speed digital imaging system.Methods:High-speed digital imaging analysis of neoglottal kinetics was performed in two patients who had undergone supracricoid laryngectomy with cricohyoidoepiglottopexy; laryngotopography, inverse filtering analysis and multiline kymography were also undertaken.Results:In case one, laryngotopography demonstrated two vibrating areas: one matched with the primary (i.e. fundamental) frequency (75 Hz) and the other with the secondary frequency (150 Hz) at the neoglottis. In case two, laryngotopography showed two vibrating areas matched with the fundamental frequency (172 Hz) at the neoglottis. The interaction between the two areas was considered to be the sound source in both patients. The waveform of the estimated volume flow at the neoglottis, obtained by inverse filtering analysis, corresponded well to the neoglottal vibration patterns derived by multiline kymography. These findings indicated that the specific sites identified at the neoglottis by the present method were likely to be the sound source in each patient.Conclusions:High-speed digital imaging analysis is effective in locating the sites responsible for voice production in patients who have undergone supracricoid laryngectomy with cricohyoidoepiglottopexy. This is the first study to clearly identify the neoglottal sound source in such patients, using a high-speed digital imaging system.

scholarly journals Islet Cryopreservation: Improved Recovery following Taurine Pretreatment

2001 ◽  
Vol 10 (3) ◽  
pp. 247-253 ◽  
Author(s):  
Anandwardhan A. Hardikar ◽  
Makarand V. Risbud ◽  
Claude Remacle ◽  
Brigitte Reusens ◽  
Joseph J. Hoet ◽  
...  
Keyword(s):  
Islet Cells ◽  
Radical Scavenging ◽  
The Body ◽  
Control Group ◽  
Image Analysis System ◽  
Islet Mass ◽  
Normal Glucose ◽  
Ethanesulfonic Acid ◽  
Analysis System ◽  
Digital Image Analysis System

Simple and efficient freezing methods with maximal postthawing recovery form the basis of ideal cryopreservation. Taurine (2-amino ethanesulfonic acid), an end-product of sulphur amino acid metabolism, is one of the most abundant free amino acids in the body. The membrane stabilizing, free radical scavenging, and osmoregulatory roles of taurine have been well documented. We studied the effect of physiological and supra-physiological concentrations (0.3 and 3.0 mM) of taurine on islet cryopreservation. Islet viability on cryopreservation was significantly improved in both the taurine-treated groups (91.9 ± 2.3% in 0.3 mM and 94.6 ± 1.58% in 3.0 mM group, p < 0.05) compared with the controls (85.7 ± 3.4%). Loss of peripheral islet cells was highly reduced in the taurine group, as examined under phase contrast and quantified by islet morphometric analysis (p < 0.05) using a digital image analysis system. Taurine-treated islets showed significant reduction in lipid peroxidation (0.905 and 0.848 nM MDA/μg protein for 0.3 and 3.0 mM taurine, respectively, p < 0.05) compared with control (1.307 nM MDA/μg protein) islets. In all, 500 islet equivalents (IE) of treated or control group islets were transplanted to BALB/c mice rendered diabetic with STZ. All animals showed a normal glucose clearance following a glucose load. Graft functionality was confirmed by normoglycemia (fasting plasma glucose: fpg < 150 mg/dl) after transplantation and reappearing hyperglycemia (fpg > 200 mg/dl) following removal of the graft. Suboptimal islet transplantation using 250 IE suggests that the grafted islet mass was inadequate for diabetes reversal. In addition, no significant differences were observed in the islet insulin content between the three groups following cryopreservation of the islets at −196°C. Our studies indicate that taurine pretreatment and its continued presence during islet cryopreservation improves the postthawing viable recovery of islets.

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