Evaluation of a Multiplex One-Step TaqMan Real-Time Reverse Transcription- PCR Assays for the Detection of H5N1 Avian Influenza Viruses in Clinical Specimens

2015 ◽  
Vol 03 (05) ◽  
Author(s):  
Stepaniuk SV Shevchuk VO
Author(s):  
C. Abolnik

A Fluorescence resonance energy transfer (FRET) real-time reverse-transcription (rRT-PCR) assay was developed that distinguishes stains of South African and European highly pathogenic (HPAI) from low pathogenicity (LPAI) H5 avian influenza viruses in the absence of virus isolation, irrespective of the length of insertion at the hemagglutinin cleavage site (H0). The assay was used to pathotype H5-type viruses detected by rRT-PCR in ostrich tracheal swabs collected during the 2006 HPAI H5N2 outbreak in the Western Cape Province.


2009 ◽  
Vol 155 (2) ◽  
pp. 193-198 ◽  
Author(s):  
Bao-Feng Qiu ◽  
Wu-Jie Liu ◽  
Da-Xin Peng ◽  
Shun-Lin Hu ◽  
Ying-Hua Tang ◽  
...  

2013 ◽  
Vol 193 (2) ◽  
pp. 452-458 ◽  
Author(s):  
Z. Kis ◽  
J. Jones ◽  
A. Creanga ◽  
K. Ferdinand ◽  
K. Inui ◽  
...  

2013 ◽  
Vol 158 (8) ◽  
pp. 1743-1753 ◽  
Author(s):  
Ilona Stefańska ◽  
Tomasz Dzieciatkowski ◽  
Lidia B. Brydak ◽  
Magdalena Romanowska

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