scholarly journals SYBR Green-Based Real-Time Reverse Transcription-PCR for Typing and Subtyping of All Hemagglutinin and Neuraminidase Genes of Avian Influenza Viruses and Comparison to Standard Serological Subtyping Tests

2011 ◽  
Vol 50 (1) ◽  
pp. 37-45 ◽  
Author(s):  
Kenji Tsukamoto ◽  
Panei Carlos Javier ◽  
Makiko Shishido ◽  
Daigo Noguchi ◽  
John Pearce ◽  
...  
Author(s):  
C. Abolnik

A Fluorescence resonance energy transfer (FRET) real-time reverse-transcription (rRT-PCR) assay was developed that distinguishes stains of South African and European highly pathogenic (HPAI) from low pathogenicity (LPAI) H5 avian influenza viruses in the absence of virus isolation, irrespective of the length of insertion at the hemagglutinin cleavage site (H0). The assay was used to pathotype H5-type viruses detected by rRT-PCR in ostrich tracheal swabs collected during the 2006 HPAI H5N2 outbreak in the Western Cape Province.


2009 ◽  
Vol 155 (2) ◽  
pp. 193-198 ◽  
Author(s):  
Bao-Feng Qiu ◽  
Wu-Jie Liu ◽  
Da-Xin Peng ◽  
Shun-Lin Hu ◽  
Ying-Hua Tang ◽  
...  

2001 ◽  
Vol 97 (1-2) ◽  
pp. 13-22 ◽  
Author(s):  
Ming-Shiuh Lee ◽  
Poa-Chun Chang ◽  
Jui-Hung Shien ◽  
Ming-Chu Cheng ◽  
Happy K Shieh

2006 ◽  
Vol 45 (2) ◽  
pp. 600-603 ◽  
Author(s):  
B. Hoffmann ◽  
T. Harder ◽  
E. Starick ◽  
K. Depner ◽  
O. Werner ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document