Comparison of Two Enzyme Immunoassays and Four Lysate Antigens for the Detection of Antibody in Canine Blastomycosis

Abstract Two solid-phase enzyme immunoassays were developed to measure 2,4-dichlorophenoxyacetic acid (2,4-D), using 2 sets of structurally distinct immunogens and enzyme ligands. The 2,4-D analog, 2-methyl- 4-chlorophenoxyacetic acid (MCPA), gave a similar response with both methods, whereas other phenoxy herbicides cross-reacted differently. In method A, the aromatic moiety of 2,4-D was distal from the carrier protein and labeled enzyme, whereas in method B, the acetic acid portion of the herbicide was distal. The use of both methods to screen for this herbicide in ground water and municipal and river water reduced the number of false-positive responses. Water sources having a low background response could be monitored with either method alone. When a concentration step, with disposable C18 extraction columns, was used, the limit of sensitivity was 5 ng/L,. Method A was the more sensitive of the 2 methods with a limit of detection of 10 j*g/L without the concentration step

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Enzyme Immunoassays for Viral Diseases

The Journal of Infectious Diseases ◽

10.1093/infdis/136.supplement_2.s274 ◽

1977 ◽

Vol 136 (Supplement 2) ◽

pp. S274-S278 ◽

Cited By ~ 31

Author(s):

D. E. Bidwell ◽

A. Bartlett ◽

A. Voller

Keyword(s):

Viral Diseases ◽

Enzyme Immunoassays

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Spread and Increase of Ratoon Stunting Disease of Sugarcane and Comparison of Disease Detection Methods

Plant Disease ◽

10.1094/pdis.1999.83.12.1170 ◽

1999 ◽

Vol 83 (12) ◽

pp. 1170-1175 ◽

Cited By ~ 25

Author(s):

J. W. Hoy ◽

M. P. Grisham ◽

K. E. Damann

Keyword(s):

Yield Loss ◽

Detection Method ◽

Xylem Sap ◽

Disease Spread ◽

Detection Methods ◽

Disease Detection ◽

Correct Identification ◽

Dot Blot ◽

Ratoon Stunting Disease ◽

Enzyme Immunoassays

The spread and increase of ratoon stunting disease (RSD) resulting from two mechanical harvests were compared in eight sugarcane cultivars at two locations. RSD spread and increase were detected in the ratoon crops grown after each harvest and varied among cultivars and locations. Disease spread and increase were greater in plants grown from stalks collected at the first harvest than in the first ratoon growth from the harvested field. RSD infection was determined using five disease detection methods: alkaline-induced metaxylem autofluorescence; microscopic examination of xylem sap; and dot blot, evaporative-binding, and tissue blot enzyme immunoassays. The tissue blot enzyme immunoassay was the most accurate RSD detection method. The dot blot and evaporative-binding enzyme immunoassays were the least sensitive for detection of RSD-infected stalks, and alkaline-induced metaxylem autofluorescence was least accurate for correct identification of noninfected stalks. The results indicate that disease spread and increase are variable even among cultivars susceptible to yield loss due to RSD, and the greatest threat of disease spread and increase occurs at planting.

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