Release of Reactive Oxygen Species by Phagocytic Cells in Response to Live Parasites in Mice Infected with Trypanosoma cruzi

1997 ◽  
Vol 56 (3) ◽  
pp. 329-334 ◽  
Author(s):  
R. L. Cardoni ◽  
C. Morales ◽  
I. Rodriguez Nantes ◽  
M. I. Antunez
Keyword(s):  
Reactive Oxygen Species ◽  
Trypanosoma Cruzi ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Phagocytic Cells

scholarly journals Proline Modulates the Trypanosoma cruzi Resistance to Reactive Oxygen Species and Drugs through a Novel D, L-Proline Transporter

PLoS ONE ◽  
2014 ◽  
Vol 9 (3) ◽  
pp. e92028 ◽  
Author(s):  
Melisa Sayé ◽  
Mariana R. Miranda ◽  
Fabio di Girolamo ◽  
María de los Milagros Cámara ◽  
Claudio A. Pereira
Keyword(s):  
Reactive Oxygen Species ◽  
Trypanosoma Cruzi ◽  
Reactive Oxygen ◽  
Oxygen Species

Induction of NADPH oxidase activity and reactive oxygen species production by a single Trypanosoma cruzi antigen

2010 ◽  
Vol 40 (13) ◽  
pp. 1531-1538 ◽  
Author(s):  
Natalia Guiñazú ◽  
Eugenio Antonio Carrera-Silva ◽  
María Cecilia Becerra ◽  
Andrea Pellegrini ◽  
Inés Albesa ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Trypanosoma Cruzi ◽  
Nadph Oxidase ◽  
Oxidase Activity ◽  
Reactive Oxygen Species Production ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Nadph Oxidase Activity ◽  
Species Production

scholarly journals Fis Is Essential for Yersinia pseudotuberculosis Virulence and Protects against Reactive Oxygen Species Produced by Phagocytic Cells during Infection

2016 ◽  
Vol 12 (9) ◽  
pp. e1005898 ◽  
Author(s):  
Erin R. Green ◽  
Stacie Clark ◽  
Gregory T. Crimmins ◽  
Matthias Mack ◽  
Carol A. Kumamoto ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Yersinia Pseudotuberculosis ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Phagocytic Cells

The function of the NADPH oxidase of phagocytes, and its relationship to other NOXs

2007 ◽  
Vol 35 (5) ◽  
pp. 1100-1103 ◽  
Author(s):  
P. Behe ◽  
A.W. Segal
Keyword(s):  
Reactive Oxygen Species ◽  
Nadph Oxidase ◽  
Reactive Oxygen ◽  
Ion Fluxes ◽  
Oxygen Species ◽  
Phagocytic Cells ◽  
Alternative Scenario ◽  
Ph Changes ◽  
Primary Function ◽  
A Charge

The NADPH oxidase of ‘professional’ phagocytic cells transfers electrons across the wall of the phagocytic vacuole, forming superoxide in the lumen. It is generally accepted that this system promotes microbial killing through the generation of reactive oxygen species and through the activity of myeloperoxidase. An alternative scenario exists in which the passage of electrons across the membrane alters the pH and generates a charge that drives ions into, and out of, the vacuole. It is proposed that the primary function of the oxidase is to produce these pH changes and ion fluxes, and the issues surrounding these processes are considered in this review. The neutrophil oxidase is the prototype of a whole family of NOXs (NAPDH oxidases) that exist throughout biology, from plants to humans, which might function, at least in part, in a similar fashion.

scholarly journals APOCYNIN, AN INHIBITOR OF THE NADPH OXIDASE COMPLEX

2020 ◽  
Vol 17 (34) ◽  
pp. 478-501
Author(s):  
Camila Nascimento GIONGO ◽  
Vanessa Falchetti LOPES ◽  
Diana Fortkamp GRIGOLETTO ◽  
Eduardo Hösel MIRANDA
Keyword(s):  
Reactive Oxygen Species ◽  
Nadph Oxidase ◽  
Superoxide Anion ◽  
Therapeutic Potential ◽  
Inflammatory Diseases ◽  
Reactive Oxygen ◽  
Enzyme Complex ◽  
Oxygen Species ◽  
Phagocytic Cells ◽  
Oxidative Species

Apocynin is a phenolic compound isolated from the plant Picrorhiza kurroa Royle ex Benth. Such a compound has been extensively investigated for its therapeutic potential in diseases involving inflammatory processes or oxidative stress due to its ability to inhibit the NADPH oxidase multienzyme complex. This complex consists of two transmembrane proteins (Nox2 and p22phox) and four cytosolic regulatory proteins (p67phox, p47phox, p40phox, and GTPase-Rac) and their activation occurs after the stimulation of phagocytic cells by the mediation of the enzyme myeloperoxidase (MPO). NADPH oxidase is the only enzyme complex that is intended for the production of superoxide anion that is precursor of highly oxidizing substances classified as reactive oxygen species (ROS). NADPH oxidase is an enzyme complex that produces superoxide anion from molecular oxygen. Ta the same time, the superoxide anion is a precursor to reactive oxygen species (ROS) catalyzed by enzymes.These oxidative species, when in excess, can induce burst, causing irreparable tissue damage. They can act by modifying the redox state of DNA, protein or lipid molecules, playing a central role in the development of chronic pathologies and various health complications. One can cite vascular problems, hyperglycemia, diabetes, hypertension, Alzheimer's disease, and cancer, among others. Apocynin, previously activated by MPO, blocks the enzyme complex and prevents the formation of these oxidative species. Therefore, the central biological function of compound is to modulate the action of NADPH oxidase, promoting a positive effect in the prevention/remediation of inflammatory diseases.

scholarly journals Galectin-1-deficient mice are protected against Trypanosoma cruzi infection through altered neutrophil migration and production of reactive oxygen species and nitric oxide

2020 ◽  
Author(s):  
Thalita Bachelli Riul ◽  
Helioswilton Sales de Campos ◽  
Djalma de Souza Lima-Junior ◽  
Ana Elisa Caleiro Seixas Azzolini ◽  
Cristina Ribeiro de Barros Cardoso ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Reactive Oxygen Species ◽  
Trypanosoma Cruzi ◽  
Neutrophil Migration ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Infection Site ◽  
Neutrophil Accumulation

SUMMARYTrypanosoma cruzi is an intracellular parasite that causes Chagas disease that affects millions of people worldwide. Many cellular and molecular aspects of this neglected disease are not fully understood. Prior studies have shown that galectin-1 (Gal-1), a β-galactoside-binding protein that regulates leukocyte recruitment to the inflammatory site, and promotes T. cruzi infection, but the mechanism is unclear. Here, we report that C57BL/6 mice lacking Gal-1 (Lgals1−/−) exhibited lower parasitemia and higher survival rates than their wildtype (WT) counterparts when infected with T. cruzi Y strain. Two weeks after infection, Lgals1−/− mice displayed greater neutrophil accumulation in infection site and heart tissue than WT mice. In T. cruzi-infected Lgals1−/− mice, infiltrated neutrophils produced increased levels of reactive oxygen species (ROS), while macrophages and neutrophils produced increased levels of nitric oxide (NO), which reduced replication and viability of parasites in vitro and downregulated IL-1β production. Pharmacological inhibition of NADPH oxidase and NO synthase during early in vivo infection reversed the protective effect of Gal-1 deficiency in Lgals1−/− mice. Together, our findings demonstrate that lacking Gal-1 favors neutrophil migration to the infection site and increases production of ROS and NO, thereby controlling the early steps of T. cruzi infection by reducing parasitemia and prolonging survival of infected mice.

Identification of Scedosporium boydii catalase A1 gene, a reactive oxygen species detoxification factor highly expressed in response to oxidative stress and phagocytic cells

2015 ◽  
Vol 119 (12) ◽  
pp. 1322-1333 ◽  
Author(s):  
Sara Mina ◽  
Cindy Staerck ◽  
Sènan M. d'Almeida ◽  
Agnès Marot ◽  
Yves Delneste ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Phagocytic Cells

scholarly journals Antichagasic Activity of Komaroviquinone Is Due to Generation of Reactive Oxygen Species Catalyzed by Trypanosoma cruzi Old Yellow Enzyme

2005 ◽  
Vol 49 (12) ◽  
pp. 5123-5126 ◽  
Author(s):  
Nahoko Uchiyama ◽  
Zakayi Kabututu ◽  
Bruno K. Kubata ◽  
Fumiyuki Kiuchi ◽  
Michiho Ito ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Life Cycle ◽  
Trypanosoma Cruzi ◽  
Reductase Activity ◽  
Reactive Oxygen ◽  
Semiquinone Radical ◽  
Oxygen Species ◽  
Old Yellow Enzyme ◽  
Interesting Candidate

ABSTRACT A novel potent trypanocidal diterpene, komaroviquinone, was reduced by Trypanosoma cruzi old yellow enzyme (TcOYE) to its semiquinone radical. The reductase activity in trypanosome lysates was completely immunoabsorbed by anti-TcOYE antibody. Since TcOYE is expressed throughout the T. cruzi life cycle, komaroviquinone is an interesting candidate for developing new antichagasic drugs.

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