ph changes
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2022 ◽  
Vol 175 ◽  
pp. 106503
Author(s):  
Zhao Jin ◽  
Da Luo ◽  
Yunlong Yu ◽  
Siqi Yang ◽  
Jing Zhang ◽  
...  

2022 ◽  
Author(s):  
Emin Zümrütdal

Abstract Yogurt is a known food item all over the world. Consuming yogurt can be quite difficult for people with lactose intolerance at times. In addition to its active carbon adsorbent feature, it is a lactose chelate. It is aimed to use yoghurt prepared by using activated carbon for nutritional purposes. For this purpose, aroma and pH changes in yogurt made with activated carbon compared to classical yogurt production were studied. It was determined that the active carbon usage method used in the study did not cause any aroma change in yoghurt, however, it increased the pH. This result makes us think that lactose cannot be used by yogurt microorganisms and that yogurt with activated carbon may be an alternative for lactose intolerant people who want to consume yogurt.


Chemosensors ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 5
Author(s):  
Chia-Ming Yang ◽  
Jia-Yuan Chang ◽  
Min-Yi Chen ◽  
Chao-Sung Lai

To evaluate point-of-care testing (POCT) for the potential early detection of biomarkers of Parkinson’s disease, a systematic investigation of portable and low-cost platforms is performed based on the Proton-enzyme-linked immunosorbent assay (Proton-ELISA) methodology. The detection of the α-synuclein antigen was first presented by biotin-relative linkers, and glucose substrate solution was first performed with a systematic experimental design to optimize the sensing results. All materials in this study are commercially available. Three different experiments with the partitional check were performed to investigate the Proton-ELISA platform, including proton catalyzed efficiency, blocking efficiency, and full Proton-ELISA procedure. The response time was selected as 15 min by the time-dependent curves of a full reaction. The limit of detection of conventional ELISA kits is 0.169 ng/mL, which is much lower than the Proton-ELISA results. The final response of the full Proton-ELISA procedure to pH changes was approximately 0.60 and 0.12 for α-synuclein antigen concentrations of 100 ng/mL and 4 ng/mL, respectively. With the partitional check, pH changes of pure glucose substrate and conjugated oxidase and interference of the nonspecific binding are 1.7 and 0.04, respectively. The lower pH changes far from the partitional check results can be concluded for the properties of glucose oxidase conjugation, including the isoelectric point and binding affinity modification by the testing environment. This preliminary guideline can be used as a lesson learnt to speed up following studies of the evaluation and optimization of other antigen detection. Therefore, Proton-ELISA can be suggested for some special applications with the help of custom-designed conjugation in the environment with less degradation or interference and a proper detection concentration range.


Gels ◽  
2021 ◽  
Vol 8 (1) ◽  
pp. 11
Author(s):  
Dmitrii G. Trofimov ◽  
Yuri I. Glazachev ◽  
Artem A. Gorodetsky ◽  
Denis A. Komarov ◽  
Tatyana V. Rybalova ◽  
...  

Local acidity and electrostatic interactions are associated both with catalytic properties and the adsorption activity of various materials, and with the vital functions of biomolecules. The observation of acid–base equilibria in stable free radicals using EPR spectroscopy represents a convenient method for monitoring pH changes and the investigation of surface electrostatics, the advantages of which are especially evident in opaque and turbid samples and in porous materials such as xerogels. Imidazoline nitroxides are the most commonly used pH-sensitive spin probes and labels due to the high sensitivity of the parameters of the EPR spectra to pH changes, their small size, and their well-developed chemistry. In this work, several new derivatives of 4-(N,N-dialkylamino)-2,5-dihydrioimidazol-1-oxyl, with functional groups suitable for specific binding, were synthesized. The dependence of the parameters of their EPR spectra on pH was studied. Several showed a pKa close to 7.4, following the pH changes in a normal physiological range, and some demonstrated a monotonous change of the hyperfine coupling constant by 0.14 mT upon pH variation by four units.


Author(s):  
Ibdal Satar ◽  
Adi Permadi ◽  
Sukma Latifatunnajib

Microbial fuel cell consisting two main components which are anode and cathode materials. In the microbial fuel cell, both anode and cathode compartments are separated with a separator. Anode generates the protons and electrons while cathode converts protons into water with the presence electrons and oxygen. During the Microbial fuel cell operation, the performance of anode is very crucial due to it provides the protons and electrons. Hence, the high efficiency microbial fuel cell is very related with the high anode performance. This work addressed to the enrichment process of electroactive bacteria (EAB) in anode of microbial fuel cell. In this work, some parameters such as current generations, , and pH changes were used to assess the enrichment process of EAB was reached. In addition, the presence of EAB on the anode surface was identified based on the morphology of anode surface. The removal of COD and the pH value were determined by using the American public health analysis method and pH tester, respectively. The morphology of anode surface was analysed by using a scanning electron microscope. Whereas, current generation was tested by using a mustimeter. The removal of COD and final pH were obtained 71.4 % and 5.7, respectively. The optimum current generation was observed 0.19 mA. The surface morphology of anode before enriched with microbes was clear surface, while after enriched with microbes was attached by microbes. The removal of COD, pH changes, current generation and morphology of anode surface could be used to assess the EAB in the anode compartment.      Keywords: Microbial fuel cell; anode; cathode; electroactive bacteria; pH changes.


Author(s):  
Laura Wimberger ◽  
Shyamal K. K. Prasad ◽  
Martin D. Peeks ◽  
Joakim Andréasson ◽  
Timothy W. Schmidt ◽  
...  
Keyword(s):  

Author(s):  
Christian W. Kuppe ◽  
Guy J D Kirk ◽  
Matthias Wissuwa ◽  
Johannes A Postma

Upland rice (Oryza sativa) is adapted to strongly phosphorus (P) sorbing soils. The mechanisms underlying P acquisition, however, are not well understood, and models typically underestimate uptake. This complicates root ideotype development and trait-based selection for further improvement. We present a novel model, which correctly simulates the P uptake by a P-efficient rice genotype measured over 48 days of growth. The model represents root morphology at the local rhizosphere scale, including root hairs and fine S-type laterals. It simulates fast-and slowly reacting soil P and the P-solubilizing effect of root-induced pH changes in the soil. Simulations predict that the zone of pH changes and P solubilization around a root spreads further into the soil than the zone of P depletion. A root needs to place laterals outside its depletion-but inside its solubilization zone to maximize P uptake. S-type laterals, which are short but hairy, appear to be the key root structures to achieve that. Thus, thicker roots facilitate the P uptake by fine lateral roots. Uptake can be enhanced through longer root hairs and greater root length density but was less sensitive to total root length and root class proportions.


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