scholarly journals DIAGNOSING INFECTION LEVELS OF FOUR HUMAN MALARIA PARASITE SPECIES BY A POLYMERASE CHAIN REACTION/LIGASE DETECTION REACTION FLUORESCENT MICROSPHERE-BASED ASSAY

2006 ◽  
Vol 74 (3) ◽  
pp. 413-421 ◽  
Author(s):  
DAVID T. McNAMARA ◽  
LAURIN J. KASEHAGEN ◽  
BRIAN T. GRIMBERG ◽  
JENNIFER COLE-TOBIAN ◽  
WILLIAM E. COLLINS ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Malaria Parasite ◽  
Parasite Species ◽  
Chain Reaction ◽  
Human Malaria Parasite ◽  
Ligase Detection Reaction ◽  
Human Malaria ◽  
Fluorescent Microsphere ◽  
Polymerase Chain

Frequency of cross-fertilization in the human malaria parasitePlasmodium falciparum

Parasitology ◽  
1993 ◽  
Vol 107 (1) ◽  
pp. 11-18 ◽  
Author(s):  
L. C. Ranford-Cartwright ◽  
P. Balfe ◽  
R. Carter ◽  
D. Walliker
Keyword(s):  
Malaria Parasite ◽  
Anopheles Stephensi ◽  
Random Mating ◽  
Crossing Experiments ◽  
Chain Reaction ◽  
Human Malaria Parasite ◽  
Human Malaria ◽  
Polymerase Chain ◽  
Cross Fertilization

SummaryTwo clones of the human malaria parasitePlasmodium falciparum, denoted 3D7 and HB3, were grownin vitrounder conditions permitting the development of gametocytes. The two clones differ in their allelic forms of two antigen genes MSP1 and MSP2. The alleles can be distinguished as size differences of polymerase chain reaction (PCR) amplified fragments of repetitive regions of each gene. Mosquitoes (Anopheles stephensi) were fed on a mixture of these gametocytes. A total of 128 oocysts was isolated from the midguts of infected mosquitoes from 9 crossing experiments between the clones. DNA extracted from these oocysts was amplified by PCR. Oocysts which contained both alleles of each gene (MSP1 and MSP2) had developed from heterozygotes produced by cross-fertilization events between 3D7 and HB3 gametes. The remaining oocysts contained single alleles of each gene, in parent clone combinations, and these had developed from homozygotes formed by self-fertilizations. The results suggest that gametes in the original mixture fed to mosquitoes had undergone random mating.

Induction of Humoral Immune Response by 66 kDa Protein and Amplification of Gene present by using Polymerase Chain Reaction

2017 ◽  
Vol 6 (6) ◽  
pp. 206-211
Author(s):  
Nisha Devi ◽  
H. S. Banal
Keyword(s):  
Polymerase Chain Reaction ◽  
Plasmodium Berghei ◽  
Genomic Dna ◽  
Malaria Parasite ◽  
Rodent Malaria Parasite ◽  
Chain Reaction ◽  
Rodent Malaria ◽  
Humoral Immune ◽  
Parasite Plasmodium ◽  
Polymerase Chain

Malaria is one of the most dreaded vector borne disease caused by apicomplexan parasite, of the genus Plasmodium. In the present study the gene which encodes 66kDa protein in rodent malaria parasite, Plasmodium berghei, was cloned. Rodent malaria parasite, Plasmodium berghei (NK-65), was found lethal to white Swiss mice (BALB/c). The immunogenicity and protective efficacy of purified 66 kDa protein was evaluated both in vitro and in vivo. Mice immunized with 66 kDa protein induced a strong humoral immune response, revealing role of 66 kDa in malaria control. Genomic DNA of P. berghei was isolated from cell free parasite. Genomic DNA serves as a template for isola on of gene encoding 66 kDa protein. Further, the gene was amplified by using polymerase chain reaction. The amplified DNA fragment of 1717 bp was extracted from agarose gel and puri ed. The purified gene was ligated to a cloning vector for cloning procedure by TA-cloning mechanism. Results of this study provide evidence that 66 kDa protein is highly immunogenic during experimental infec on in rodents and such an gen will be a suitable candidate malaria vaccine. 

Sign in / Sign up

Export Citation Format

Share Document