Effect of entomopathogenic nematodes under laboratory conditions on <i>Cylas puncticollis</i> pest of sweet potato in Benin

The virulence of ten indigenous and two commercial isolates of entomopathogenic nematodes against the black cutworm, Agrotis ipsilon, was assessed under laboratory conditions. When comparing commercial isolates, Steinernema carpocapsae exhibited higher virulence than S. feltiae. One indigenous isolate of S. carpocapsae (6Sc) provided similar or higher virulence than the commercial isolate against black cutworm larvae. An indigenous strain of S. kraussei demonstrated low virulence in our study.

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Can pre-colonisation of the soil substrate increase the efficacy of entomopathogenic nematodes (Rhabditida: Steinernematidae)?

Journal of Agrobiology ◽

10.2478/s10146-009-0004-1 ◽

2010 ◽

Vol 27 (1) ◽

pp. 19-25

Author(s):

Štěpánka Radová

Keyword(s):

Entomopathogenic Nematodes ◽

Tenebrio Molitor ◽

Steinernema Feltiae ◽

Soil Substrate ◽

Laboratory Conditions ◽

Positive Effect

Can pre-colonisation of the soil substrate increase the efficacy of entomopathogenic nematodes (Rhabditida: Steinernematidae)?The effects of soil pre-colonisation with entomopathogenic nematodes Steinernema feltiae, S. carpocapsae and S. arenarium on the mortality of Tenebrio molitor larvae were tested under laboratory conditions. The soil was inhabited with the nematodes for 7 days prior to the addition of the larvae. Nematode concentrations of 50 and 500 infective juveniles per box were used. S. carpocapsae reacted to pre-colonisation negatively and the efficacy decreased at the concentrations tested. S. arenarium showed generally low efficacy against Tenebrio molitor larvae and pre-colonisation showed only a slight positive effect on the efficacy of this species. S. feltiae increased its efficacy at the concentration of 500 infective juveniles per box compared with other tested nematodes.

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Evaluation of two native entomopathogenic nematodes against Odontotermes obesus (Rambur) (Isoptera: Termitidae) and Agrotis ipsilon (Hufnagel) (Lepidoptera: Noctuidae)

Egyptian Journal of Biological Pest Control ◽

10.1186/s41938-021-00457-8 ◽

2021 ◽

Vol 31 (1) ◽

Author(s):

K. Sindhura Bhairavi ◽

Badal Bhattacharyya ◽

Gitanjali Devi ◽

Sudhansu Bhagawati ◽

Partha Pratim Gyanudoy Das ◽

...

Keyword(s):

Biological Control ◽

Mortality Rate ◽

Entomopathogenic Nematodes ◽

Heterorhabditis Bacteriophora ◽

Agrotis Ipsilon ◽

Biological Control Agents ◽

Laboratory Conditions ◽

Odontotermes Obesus ◽

Percent Mortality ◽

Lepidoptera Noctuidae

Abstract Background Entomopathogenic nematodes (EPNs) are one of the widely studied biological control agents. The present study was conducted to evaluate the efficacy of two EPNs species, Heterorhabditis bacteriophora (Poinar) (Rhabditida: Heterorhabditidae) and Steinernema aciari (Qui, Yan, Zhou, Nguyen and Pang) (Rhabditida: Steinernematidae), isolated locally from soils of Majuli river island, Assam, India against two important subterrenean pests; Odontotermes obesus (Rambur) (Isoptera: Termitidae) and Agrotis ipsilon (Hufnagel) (Lepidoptera: Noctuidae) under laboratory conditions. Results In case of O. obesus, mortality percent was recorded by H. bacteriophora after 72 h. at 300 IJs/termite and by S. aciari at 250 and 300 IJs/termite after 96 h. The lowest LD50 and LT50 values obtained for H. bacteriophora were 13.054 IJs/termite and 26.639 h., respectively, while those of S. aciari were 42.040 IJs/termite and 31.761 h., respectively. With respect to A. ipsilon, H. bacteriophora registered a highest mortality rate at 300 IJs/larvae after 144 h. S. aciari showed 100 percent mortality at 300 IJs/larva after 168 h. The lowest values of LD50 and LT50 for H. bacteriophora were 35.711 IJs/larva and 83.050 h., respectively. The lowest values of LD50 and LT50 for S. aciari were 71.192 IJs/larvae and 97.921 h., respectively. Overall, H. bacteriophora displayed more virulence toward O. obesus and A. ipsilon than S. aciari. Conclusion Both native EPNs were found effective against O. obesus and A. ipsilon. However, H. bacteriophora was more virulent toward O. obesus and A. ipsilon than S. aciari under the laboratory conditions.

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