scholarly journals Efficacy of autologous bone marrow mononuclear cell transplantation in dogs with chronic spinal cord injury

2020 ◽  
Vol 10 (2) ◽  
pp. 206-215
Author(s):  
Katsutoshi Tamura ◽  
Noritaka Maeta
Keyword(s):  
Spinal Cord ◽  
Bone Marrow ◽  
Spinal Cord Injury ◽  
Cell Transplantation ◽  
Mononuclear Cell ◽  
Motor Neurons ◽  
Research Work ◽  
Autologous Bone Marrow ◽  
Bone Marrow Mononuclear Cell ◽  
Cord Injury

Background: Spinal cord injury (SCI) is relatively common in dogs and is a devastating condition involving loss of sensory neurons and motor  neurons. However, the main clinical protocol for the management of SCI is surgery to decompress and stabilize the vertebra. Cell transplantation therapy is a very promising strategy for the treatment of chronic SCI, but extensive preclinical and clinical research work remains.Aim: The aim of this study is to confirm the effect of bone marrow-derived mononuclear cell (BM-MNC) transplantation for chronic SCI in dogs.Methods: We tested the treatment efficiency of chronic SCI in 12 dogs using BM-MNC transplantation. Neurological evaluation used the Texas Spinal Cord Injury Scale (TSCIS). Concurrently, we characterized the transplanted cells by evaluation using quantitative real-time polymerase chain reaction, flow cytometry, and enzyme-linked immunosorbent assay.Result: All dogs had a pre-transplantation TSCIS score of 0. Two animals did not show any improvement in their final TSCIS scores. The remaining 10 dogs (83.4%) achieved improvement in the final TSCIS scores. Five of them (41.7%) regained ambulatory function with a TSCIS score greater than 10. We determined that canine BM-MNCs expressed hepatocyte growth factor (HGF) mRNA at higher levels than other cytokines, with significant  increases in HGF levels in cerebrospinal fluid within 48 hours after autologous BM-MNC transplantation into the subarachnoid space of the spinal dura matter in dogs.Conclusions: BM-MNC transplantation may be effective for at least some cases of chronic SCI. Keywords: Bone marrow-derived mononuclear cell, Cell therapy, Spinal cord injury.

Safety of Autologous Bone Marrow Stromal Cell Transplantation in Dogs with Acute Spinal Cord Injury

2012 ◽  
Vol 41 (4) ◽  
pp. 437-442 ◽  
Author(s):  
Hidetaka Nishida ◽  
Masanari Nakayama ◽  
Hiroshi Tanaka ◽  
Masahiko Kitamura ◽  
Shingo Hatoya ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Bone Marrow ◽  
Spinal Cord Injury ◽  
Cell Transplantation ◽  
Stromal Cell ◽  
Bone Marrow Stromal Cell ◽  
Autologous Bone Marrow ◽  
Marrow Stromal Cell ◽  
Cord Injury ◽  
Autologous Bone

Bone marrow-derived mononuclear cell transplantation in spinal cord injury patients by lumbar puncture

2014 ◽  
Vol 32 (4) ◽  
pp. 473-482 ◽  
Author(s):  
Yoshihisa Suzuki ◽  
Namiko Ishikawa ◽  
Kaoru Omae ◽  
Tatsuya Hirai ◽  
Katsunori Ohnishi ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Bone Marrow ◽  
Spinal Cord Injury ◽  
Lumbar Puncture ◽  
Cell Transplantation ◽  
Mononuclear Cell ◽  
Cord Injury

scholarly journals Autologous bone marrow cell transplantation in acute spinal cord injury—an Indian pilot study

Spinal Cord ◽  
2015 ◽  
Vol 54 (1) ◽  
pp. 57-64 ◽  
Author(s):  
H S Chhabra ◽  
K Sarda ◽  
M Arora ◽  
R Sharawat ◽  
V Singh ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Bone Marrow ◽  
Spinal Cord Injury ◽  
Pilot Study ◽  
Cell Transplantation ◽  
Bone Marrow Cell ◽  
Marrow Cell ◽  
Autologous Bone Marrow ◽  
Cord Injury ◽  
Autologous Bone

scholarly journals CD157 in bone marrow mesenchymal stem cells mediates mitochondrial production and transfer to improve neuronal apoptosis and functional recovery after spinal cord injury

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Jing Li ◽  
Heyangzi Li ◽  
Simin Cai ◽  
Shi Bai ◽  
Huabo Cai ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Bone Marrow ◽  
Spinal Cord Injury ◽  
Mesenchymal Stem Cells ◽  
Motor Neurons ◽  
Functional Recovery ◽  
Mitochondrial Transfer ◽  
Cord Injury ◽  
Marrow Mesenchymal Stem Cells

Abstract Background Recent studies demonstrated that autologous mitochondria derived from bone marrow mesenchymal stem cells (BMSCs) might be valuable in the treatment of spinal cord injury (SCI). However, the mechanisms of mitochondrial transfer from BMSCs to injured neurons are not fully understood. Methods We modified BMSCs by CD157, a cell surface molecule as a potential regulator mitochondria transfer, then transplanted to SCI rats and co-cultured with OGD injured VSC4.1 motor neuron. We detected extracellular mitochondrial particles derived from BMSCs by transmission electron microscope and measured the CD157/cyclic ADP-ribose signaling pathway-related protein expression by immunohistochemistry and Western blotting assay. The CD157 ADPR-cyclase activity and Fluo-4 AM was used to detect the Ca2+ signal. All data were expressed as mean ± SEM. Statistical analysis was analyzed by GraphPad Prism 6 software. Unpaired t-test was used for the analysis of two groups. Multiple comparisons were evaluated by one-way ANOVA or two-way ANOVA. Results CD157 on BMSCs was upregulated when co-cultured with injured VSC4.1 motor neurons. Upregulation of CD157 on BMSCs could raise the transfer extracellular mitochondria particles to VSC4.1 motor neurons, gradually regenerate the axon of VSC4.1 motor neuron and reduce the cell apoptosis. Transplantation of CD157-modified BMSCs at the injured sites could significantly improve the functional recovery, axon regeneration, and neuron apoptosis in SCI rats. The level of Ca2+ in CD157-modified BMSCs dramatically increased when objected to high concentration cADPR, ATP content, and MMP of BMSCs also increased. Conclusion The present results suggested that CD157 can regulate the production and transfer of BMSC-derived extracellular mitochondrial particles, enriching the mechanism of the extracellular mitochondrial transfer in BMSCs transplantation and providing a novel strategy to improve the stem cell treatment on SCI.

Efficacy and safety of bone marrow-derived cell transplantation for spinal cord injury: a systematic review and meta-analysis of clinical trials

2015 ◽  
Vol 29 (9) ◽  
pp. 786-795 ◽  
Author(s):  
Xiao Chuan Li ◽  
Cheng Fan Zhong ◽  
Gui Bin Deng ◽  
Rong Wei Liang ◽  
Chun Ming Huang
Keyword(s):  
Systematic Review ◽  
Spinal Cord ◽  
Bone Marrow ◽  
Spinal Cord Injury ◽  
Clinical Trials ◽  
Cell Transplantation ◽  
Meta Analysis ◽  
Efficacy And Safety ◽  
Cord Injury

scholarly journals Autologous Bone Marrow-Derived Cell Therapy Combined with Physical Therapy Induces Functional Improvement in Chronic Spinal Cord Injury Patients

2014 ◽  
Vol 23 (6) ◽  
pp. 729-745 ◽  
Author(s):  
Wael Abo El-Kheir ◽  
Hala Gabr ◽  
Mohamed Reda Awad ◽  
Osama Ghannam ◽  
Yousef Barakat ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Bone Marrow ◽  
Spinal Cord Injury ◽  
Physical Therapy ◽  
Cell Therapy ◽  
Autologous Bone Marrow ◽  
Functional Improvement ◽  
Chronic Spinal Cord Injury ◽  
Cord Injury ◽  
Autologous Bone

Autologous bone marrow derived stem cell therapy in traumatic spinal cord injury: A case report and review of literature

2014 ◽  
Vol 11 (2) ◽  
pp. 150-153
Author(s):  
Pradipta Tripathy ◽  
Chidananda Dash ◽  
Aurobind Rath ◽  
S. Chakraborty ◽  
Jagannath Sahoo ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Bone Marrow ◽  
Spinal Cord Injury ◽  
Stem Cell ◽  
Case Report ◽  
Autologous Bone Marrow ◽  
Traumatic Spinal Cord Injury ◽  
Review Of Literature ◽  
Cord Injury ◽  
Autologous Bone
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