scholarly journals Nanoflares: Probes for Transfection and mRNA Detection in Living Cells*

2020 ◽  
pp. 1717-1726
Author(s):  
Dwight S. Seferos ◽  
David A. Giljohann ◽  
Andrew E. Prigodich ◽  
Chad A. Mirkin
Keyword(s):  
Living Cells ◽  
Mrna Detection

scholarly journals SunRISE: long-term imaging of individual mRNA molecules in living cells

2020 ◽  
Author(s):  
Yue Guo ◽  
Robin E. C. Lee
Keyword(s):  
Phase Separation ◽  
Single Cell ◽  
Cell Imaging ◽  
Living Cells ◽  
Mrna Detection ◽  
Central Dogma ◽  
Single Cell Imaging ◽  
Cell Fixation ◽  
Limited Sensitivity

AbstractSingle-cell imaging of individual mRNAs has revealed core mechanisms of the central dogma. However, most approaches require cell fixation or have limited sensitivity for live-cell applications. Here, we describe SunRISE (SunTag-based Reporter for Imaging Signal Enriched mRNA), a computationally and experimentally optimized approach for unambiguous single-mRNA detection in living cells. We demonstrate SunRISE with long-term epifluorescence imaging, using translational stress to track mRNA phase separation and recovery from cytosolic droplets.

scholarly journals Nanoflares: Probes for Transfection and mRNA Detection in Living Cells*

2020 ◽  
pp. 1717-1726
Author(s):  
Dwight S. Seferos ◽  
David A. Giljohann ◽  
Andrew E. Prigodich ◽  
Chad A. Mirkin
Keyword(s):  
Living Cells ◽  
Mrna Detection

mRNA detection in living cells: A next generation cancer stem cell identification technique

Methods ◽  
2015 ◽  
Vol 82 ◽  
pp. 47-54 ◽  
Author(s):  
Steven McClellan ◽  
Jaroslav Slamecka ◽  
Patrick Howze ◽  
Lee Thompson ◽  
Michael Finan ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cancer Stem Cell ◽  
Living Cells ◽  
Next Generation ◽  
Mrna Detection ◽  
Cell Identification ◽  
Identification Technique

scholarly journals A Targeted, Self-Delivered, and Photocontrolled Molecular Beacon for mRNA Detection in Living Cells

2013 ◽  
Vol 135 (35) ◽  
pp. 12952-12955 ◽  
Author(s):  
Liping Qiu ◽  
Cuichen Wu ◽  
Mingxu You ◽  
Da Han ◽  
Tao Chen ◽  
...  
Keyword(s):  
Molecular Beacon ◽  
Living Cells ◽  
Mrna Detection

scholarly journals Fluorescence Lifetime Imaging of Nanoflares for mRNA Detection in Living Cells

2016 ◽  
Vol 88 (4) ◽  
pp. 1979-1983 ◽  
Author(s):  
Jing Shi ◽  
Ming Zhou ◽  
Aihua Gong ◽  
Qijun Li ◽  
Qian Wu ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Living Cells ◽  
Fluorescence Lifetime Imaging ◽  
Mrna Detection ◽  
Lifetime Imaging

Dual-channel signals for intracellular mRNA detection via a PRET nanosensor

2017 ◽  
Vol 53 (55) ◽  
pp. 7768-7771 ◽  
Author(s):  
Tao Xie ◽  
Meng Li ◽  
Yi-Tao Long
Keyword(s):  
False Positive ◽  
Living Cells ◽  
Mrna Detection ◽  
Dual Channel ◽  
Positive Results

An intracellular nanosensor was designed and developed to accurately sense mRNA in living cells without false positive results.

scholarly journals Nano-Flares:  Probes for Transfection and mRNA Detection in Living Cells

2007 ◽  
Vol 129 (50) ◽  
pp. 15477-15479 ◽  
Author(s):  
Dwight S. Seferos ◽  
David A. Giljohann ◽  
Haley D. Hill ◽  
Andrew E. Prigodich ◽  
Chad A. Mirkin
Keyword(s):  
Living Cells ◽  
Mrna Detection

scholarly journals Nanoflares: Probes for Transfection and mRNA Detection in Living Cells*

2020 ◽  
pp. 1717-1726
Author(s):  
Dwight S. Seferos ◽  
David A. Giljohann ◽  
Haley D. Hill ◽  
Andrew E. Prigodich ◽  
Chad A. Mirkin
Keyword(s):  
Living Cells ◽  
Mrna Detection

Photoactivated Specific mRNA Detection in Single Living Cells by Coupling “Signal-on” Fluorescence and “Signal-off” Electrochemical Signals

Nano Letters ◽  
2018 ◽  
Vol 18 (8) ◽  
pp. 5116-5123 ◽  
Author(s):  
Fujian Huang ◽  
Meihua Lin ◽  
Ruilin Duan ◽  
Xiaoding Lou ◽  
Fan Xia ◽  
...  
Keyword(s):  
Living Cells ◽  
Mrna Detection ◽  
Single Living Cells ◽  
Single Living ◽  
Specific Mrna
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