Death and Loss

2021 ◽  
pp. 116-124
Author(s):  
Nicholas Mazza
Keyword(s):  
Death And Loss

Evaluation of radiation-induced genotoxicity on hu-man melanoma cells (SK-MEL-37) by flow cytometry

2019 ◽  
Vol 7 (2A) ◽  
Author(s):  
Leticia Bonfim ◽  
Luma Ramirez de Carvalho ◽  
Daniel Perez Vieira
Keyword(s):  
Genetic Material ◽  
Micronucleus Assay ◽  
Melanoma Cells ◽  
Dead Cell ◽  
Genotoxic Damage ◽  
Sytox Green ◽  
60Co Source ◽  
Radiation Induced ◽  
Death And Loss ◽  
Cell Fractions

Micronucleus assay is a test used to evaluate genotoxic damage in cells, which can be caused by various factors, like ionizing radiation. Interactions between radiation energies and DNA can cause breakage, leading to use chromosomal mutations or loss of genetic material, important events that could be induced in solid tumors to mitigate its expansion within human body.  Melanoma has been described as a tumor with increased radio resistance.  This work evaluated micronuclei percentages (%MN) in human melanoma cells (SK-MEL-37), irradiated by gamma radiation, with doses between 0 and 16Gy.  Cell suspensions were irradiated in PBS by a 60Co source in doses between 0 and 16Gy, and incubated by 48h.  Then cell membranes were lysed in the presence of SYTOX Green and EMA dyes, preserving nuclear membranes.  Using this method, EMA-stained nuclei could be discriminated as those derived from dead cells, and SYTOX nuclei and micronuclei could be quantified.  Micronuclei percentages were found to be proportional to dose, (R2 = 0.997).  Only the highest dose (16Gy) could induce statistically significant increase of MN (p<0.0001), although cultures irradiated by 4, 8 and 16Gy showed significant increase of dead cell fractions.  Calculation of the nuclei-to-beads ratio showed that 8 and 16Gy could reduce melanoma cell proliferation.  Results showed that although cell death and loss of proliferative capacity could be observed on cultures irradiated at lower doses, genotoxic damage could be induced only on a higher dose. Resistance to radiation-induced genotoxicity could explain a relatively high radio resistance of melanoma tumors.

scholarly journals Learning to Live Through Loss: Grief and the Mourning Process

EDIS ◽  
2006 ◽  
Vol 2006 (26) ◽  
Author(s):  
Carolyn S. Wilken
Keyword(s):  
Fact Sheet ◽  
Death And Loss ◽  
Mourning Process

FCS-2267, a 5-page fact sheet by Carolyn Wilken, is one in a series of six publications dealing with grief, death, and loss. This publication gives detailed descriptions of the stages of grief and explains how different kinds of deaths may cause different reactions. Also gives specific guidance on how to help someone who is grieving. Published by the UF Department of Family, Youth and Community Sciences, November 2006.

Appartenenza e separazione nelle terapie del lutto: da un tempo bloccato alla presenza di un'assenza

2009 ◽  
pp. 5-21
Author(s):  
Anna Maria Rapone ◽  
Trasarti Wilma Sponti
Keyword(s):  
Personal Experience ◽  
Case Reports ◽  
Personal Resources ◽  
Past Century ◽  
Urban Context ◽  
Private Space ◽  
Death And Loss

- Rituals, of past century culture, allowed grieving to be socially expressed, thus facilitating its condivision and elaboration. Now day culture, in occidental urban context, often leaves in a private space, psychotherapy, the expression of it. The authors, from their personal experience of psychotherapists and teachers, individuate in block of time and in a blocking of grieving a possible source of psychopathology. Aim of this article is to offer a few points of reflection on the theme of grieving for a wider understanding and utilization of it. Then they suggest the concept of loyalty, resilience, together with the concept of pacing, to accept death and loss in an isomorphic possibility of individuation and personal resources utilization. The elaboration of grief can then evolve in a nurturing way. Case reports illustrate their experience on grief elaboration.

Gaming and Grieving: Digital Games as Means of Confronting and Coping with Death

2020 ◽  
Vol 9 (3) ◽  
pp. 326-346
Author(s):  
Beverley Foulks McGuire
Keyword(s):  
Human Body ◽  
Digital Games ◽  
Digital Game ◽  
Air Bubble ◽  
Mountainous Landscapes ◽  
Game Environment ◽  
Death And Loss ◽  
And Coping ◽  
Coping With Death

Abstract This paper explores structural similarities between playing a digital game and experiencing grief. The digital game Mandagon evokes a sense of loss through its game environment of grey mountainous landscapes, broken wooden scaffolds, and Tibetan temples and prayer flags in states of disrepair. It elicits feelings of disorientation and dependency as players repeatedly fall from scaffolds but ascend by using lifts or finding air bubble streams underwater. It encompasses terrestrial, corporeal, and cosmic crossings as players move through air, land, and water, as they neither inhabit nor encounter a human body, and they cross various cosmic thresholds through the course of the game. For players struggling with grief, it validates and normalizes feelings of emptiness, loneliness, and vulnerability in the wake of death and loss.

Death and Loss

2021 ◽  
pp. 257-295
Author(s):  
Colleen T. Fogarty
Keyword(s):  
Death And Loss

scholarly journals Gastrointestinal bleeding during acute ischaemic stroke hospitalisation increases the risk of stroke recurrence

2020 ◽  
Vol 5 (2) ◽  
pp. 116-120
Author(s):  
Wanliang Du ◽  
Xingquan Zhao ◽  
Yilong Wang ◽  
Yuesong Pan ◽  
Gaifen Liu ◽  
...  
Keyword(s):  
Ischaemic Stroke ◽  
Acute Ischaemic Stroke ◽  
Patient Characteristics ◽  
Recurrence Risk ◽  
Stroke Recurrence ◽  
Gi Bleeding ◽  
Stroke Registry ◽  
Risk Of Death ◽  
Increased Risk ◽  
Death And Loss

ObjectiveGastrointestinal (GI) bleeding in patients who had a stroke is strongly associated with a higher risk of death and loss of independence. However, it is unknown whether GI bleeding increases risk for recurrence of stroke. In this study, we assess the potential relationship between GI bleeding and stroke recurrence in patients within 12 months of an acute ischaemic stroke (AIS), using the China National Stroke Registry (CNSR).MethodsThis study included 22 216 patients who had an ischaemic stroke included in the CNSR from 2007 to 2008. We analysed baseline patient characteristics, GI bleeding and outcomes of patients who had an AIS, specifically stroke recurrence at 3, 6 and 12 months. We used multivariable logistic regression to evaluate a possible association between GI bleeding and stroke recurrence.ResultsOf the 12 415 patients included in our study, 12.3%, 15.5% and 17.7% had a stroke recurrence at 3, 6 and 12 months, respectively. GI bleeding was an independent stroke recurrence risk factor in patients after ischaemic stroke at 3 months (adjusted OR 1.481, 95% CI 1.118 to 1.962), 6 months (adjusted OR 1.448, 95% CI 1.106 to 1.896) and 12 months (adjusted OR 1.350; 95% CI 1.034 to 1.763).ConclusionGI bleeding was associated with the increased risk of stroke recurrence after an AIS.

Differential effects of EGF on repair of cellular functions after dichlorovinyl-l-cysteine-induced injury

1999 ◽  
Vol 276 (2) ◽  
pp. F228-F236 ◽  
Author(s):  
Graz˙yna Nowak ◽  
Kenneth B. Keasler ◽  
Douglas E. McKeller ◽  
Rick G. Schnellmann
Keyword(s):  
Cell Death ◽  
Oxygen Consumption ◽  
Atpase Activity ◽  
Glucose Uptake ◽  
Mitochondrial Function ◽  
Cellular Functions ◽  
Sublethal Injury ◽  
Epidermal Growth ◽  
Death And Loss ◽  
Over Time

This study examined the repair of renal proximal tubule cellular (RPTC) functions following sublethal injury induced by the nephrotoxicant S-(1,2-dichlorovinyl)-l-cysteine (DCVC). DCVC exposure resulted in 31% cell death and loss 24 h following the treatment. Monolayer confluence recovered through migration/spreading but not proliferation after 6 days. Basal, uncoupled, and ouabain-sensitive oxygen consumption (Qo 2) decreased 47, 76, and 62%, respectively, 24 h after DCVC exposure. Na+-K+-ATPase activity and Na+-dependent glucose uptake were inhibited 80 and 68%, respectively, 24 h after DCVC exposure. None of these functions recovered over time. Addition of epidermal growth factor (EGF) following DCVC exposure did not prevent decreases in basal, uncoupled, and ouabain-sensitive Qo 2 values and Na+-K+-ATPase activity but promoted their recovery over 4–6 days. In contrast, no recovery of Na+-dependent glucose uptake occurred in the presence of EGF. These data show that: 1) DCVC exposure decreases mitochondrial function, Na+-K+-ATPase activity, active Na+ transport, and Na+-dependent glucose uptake in sublethally injured RPTC; 2) DCVC-treated RPTC do not proliferate nor regain their physiological functions in this model; and 3) EGF promotes recovery of mitochondrial function and active Na+ transport but not Na+-dependent glucose uptake. These results suggest that cysteine conjugates may cause renal dysfunction, in part, by decreasing RPTC functions and inhibiting their repair.

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