scholarly journals Conceptualization and validation of an innovative direct immunofluorescence technique utilizing fluorescein conjugate against IgG + IgG4 for routinely diagnosing autoimmune bullous dermatoses

2021 ◽  
Vol 46 (2) ◽  
pp. 183-190
Author(s):  
Magdalena Danuta Jałowska ◽  
Justyna Gornowicz-Porowska ◽  
Agnieszka Seraszek-Jaros ◽  
Monika Bowszyc-Dmochowska ◽  
Elżbieta Kaczmarek ◽  
...  
Keyword(s):  
Direct Immunofluorescence ◽  
Immunofluorescence Technique

scholarly journals Reproducibility of interpretation of the test for antibody-coated bacteria in urinary sediment

1977 ◽  
Vol 6 (4) ◽  
pp. 359-361
Author(s):  
D R Schaberg ◽  
R W Haley ◽  
P M Terry ◽  
J E McGowan
Keyword(s):  
Sensitivity And Specificity ◽  
Interobserver Variability ◽  
Direct Immunofluorescence ◽  
Immunofluorescence Technique ◽  
Urinary Sediment ◽  
Majority Opinion ◽  
Reading System ◽  
Individual Reading

The direct immunofluorescence technique for detecting antibody-coated bacteria in urinary sediment is felt to be useful in distinguishing infection of the kidney from infection of the bladder. An independent, blind multiple-reading system was used to measure interobserver variability in the evaluation of slides of urinary sediments for antibody-coated bacteria. Three independent observers agreed unanimously on first reading in 88% of 253 specimens. When compared with the majority opinion, the sensitivity and specificity of an individual reading were 91 and 95%, respectively.

Origin of Kallikrein in Rat and Human Exocrine Glands and Kidney

1979 ◽  
Vol 57 (s5) ◽  
pp. 239s-241s ◽  
Author(s):  
T. B. Ørstavik ◽  
K. Nustad ◽  
P. Brandtzaeg
Keyword(s):  
Cellular Localization ◽  
Rat Kidney ◽  
Acinar Cells ◽  
Human Kidney ◽  
Direct Immunofluorescence ◽  
Human Pancreas ◽  
Exocrine Glands ◽  
Duct System ◽  
Immunofluorescence Technique ◽  
Tubular Cells

1. The cellular localization of kallikrein was investigated in rat and human exocrine glands and kidney by a direct immunofluorescence technique. 2. Kallikrein was found in the duct system of the rat and human major salivary glands. 3. Kallikrein was found in the distal tubular cells of the rat kidney. Attempts to localize kallikrein in the human kidney were unsuccessful. 4. In the rat and human pancreas, kallikrein was found as a pro-enzyme in the acinar cells.

scholarly journals Studies on Tyzzer's disease : application of immunofluorescence for detection of Bacillus piliformis and for demonstration and determination of antibodies to it in sera from mice and rabbits

1977 ◽  
Vol 11 (2) ◽  
pp. 69-73 ◽  
Author(s):  
A. Schaich Fries
Keyword(s):  
Infected Mouse ◽  
Indirect Immunofluorescence ◽  
Mouse Serum ◽  
Direct Immunofluorescence ◽  
Subclinical Infection ◽  
Immunofluorescence Technique ◽  
Serum Antibodies ◽  
Indirect Immunofluorescence Technique

Bacillus pilifarmis antigens were demonstrated in smear preparations from infected mouse livers by direct immunofluorescence technique. Mouse serum antibodies against B. pilifarmis were demonstrated by indirect immunofluorescence technique. The test was employed quantitatively both on sera from experimentally infected mice and on sera from clinically healthy mice from colonies infected with B. pilifarmis, and could be used for the quantitative demonstration of antibodies in sera from a stock of rabbits with Tyzzer's disease. It was found very useful for the detection of subclinical infection.

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