Comparison of Culture, Direct Immunofluorescence Assay, and Multiplex Reverse Transcriptase PCR for Detection of Respiratory Viruses

2011 ◽  
Vol 1 (4) ◽  
pp. 221 ◽  
Author(s):  
Kui Hyun Yoon ◽  
Ji Hyun Cho
Keyword(s):  
Reverse Transcriptase ◽  
Respiratory Viruses ◽  
Immunofluorescence Assay ◽  
Direct Immunofluorescence ◽  
Reverse Transcriptase Pcr ◽  
Direct Immunofluorescence Assay

scholarly journals Evaluation of R-Mix FreshCells in Shell Vials for Detection of Respiratory Viruses

2000 ◽  
Vol 38 (12) ◽  
pp. 4660-4662 ◽  
Author(s):  
Caroline K. Y. Fong ◽  
Mi Kyung Lee ◽  
Brigitte P. Griffith
Keyword(s):  
Influenza A Virus ◽  
Cell Lines ◽  
A549 Cell ◽  
Influenza A ◽  
Respiratory Viruses ◽  
Immunofluorescence Assay ◽  
Direct Immunofluorescence ◽  
Conventional Culture ◽  
Direct Immunofluorescence Assay ◽  
High Degree

The performance of a mixture of mink lung and A549 cell lines in shell vials (MSVs) for the detection of respiratory viruses in 159 specimens was evaluated. MSVs, conventional culture, and direct immunofluorescence assay identified 96, 85, and 67% of the influenza A virus-positive specimens, respectively. MSVs provided both a high degree of sensitivity and rapid turnaround times for the detection of influenza A virus.

scholarly journals Etiology, clinical characteristics and coinfection status of bronchiolitis in Suzhou

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Jiahong Tan ◽  
Jinfeng Wu ◽  
Wujun Jiang ◽  
Li Huang ◽  
Wei Ji ◽  
...  
Keyword(s):  
Virus Infection ◽  
Clinical Characteristics ◽  
Immunofluorescence Assay ◽  
Clinical Syndrome ◽  
Enzyme Linked Immunosorbent Assay ◽  
Direct Immunofluorescence ◽  
Increased Risk ◽  
Syncytial Virus ◽  
Direct Immunofluorescence Assay ◽  
Mixed Virus Infection

Abstract Background Bronchiolitis is a clinical syndrome commonly encountered in practice, particularly among infants and young children. To investigate the prevalence of pathogens in hospitalized children with bronchiolitis and study the clinical characteristics of bronchiolitis with or without coinfections. Methods We investigated the respiratory specimens and clinical data of 1012 children with bronchiolitis who were treated at the Children’s Hospital of Soochow University between November 2011 and December 2018. The nasopharyngeal aspirates were examined to detect viruses by direct immunofluorescence assay or polymerase chain reaction (PCR). Mycoplasma pneumoniae (MP) was tested by PCR and enzyme-linked immunosorbent assay. Results Of the 1134 children less than 2 years with bronchiolitis, 122 were excluded by exclusion criteria. Causative pathogen was detected in 83.2% (842 of 1012). The majority of these (614 [72.9%] of 842) were single virus infection. The most common pathogens detected were respiratory syncytial virus (RSV) (44.4%), MP (15.6%), and human rhinovirus (HRV) (14.4%). Coinfection was identified in 13.5% (137 of 1012) of the patients. Coinfection included mixed virus infection and virus infection with MP infection. Children with single virus infection had a higher rate of oxygen therapy compared with single MP infection. Conclusions The most common pathogen detected in children with bronchiolitis is RSV, followed by MP and HRV. Coinfection leads to a longer period of illness, increased severity of the symptoms and increased risk of hypoxemia.

scholarly journals HLA-B27 phenotyping with flow cytometry: further improvement by multiple monoclonal antibodies

1997 ◽  
Vol 43 (10) ◽  
pp. 1975-1981 ◽  
Author(s):  
Johannes J M L Hoffmann ◽  
Willy C M Janssen
Keyword(s):  
Flow Cytometry ◽  
Internal Control ◽  
Hla Antigens ◽  
Immunofluorescence Assay ◽  
Direct Immunofluorescence ◽  
Hla B27 ◽  
Roc Curve Analysis ◽  
Cross Reactions ◽  
Flow Cytometric Method ◽  
Direct Immunofluorescence Assay

Abstract To establish the optimal flow cytometric method for HLA-B27 phenotyping, we compared several strategies, using three monoclonal anti-B27 antibodies (from the HLA-ABC-m3, GS145.2, and FD705 clones). We used a triple-color direct immunofluorescence assay, including a T-lymphocyte-specific antibody as an internal control and an anti-HLA-Bw4 antibody. Blood samples from >400 subjects were tested. From ROC curve analysis none of the three antibodies appeared to be suitable for use as a single typing reagent. The efficiency of the test was affected by cross-reactions with other HLA antigens, notably the HLA-B7 antigen. Preincubation with anti-B7 serum efficiently inhibited this cross-reaction and raised the test efficiency considerably. We concluded that none of the anti-B27 antibodies investigated is suitable for use as a single typing reagent. Additional typing of Bw4 is not valuable, whereas inhibition of cross-reactions due to the B7 antigen will considerably improve the performance of the test. We recommend that two different monoclonal anti-B27 antibodies be used for accurate and reliable HLA-B27 phenotyping with flow cytometry.

scholarly journals Urban park-related risks forGiardiaspp. infection in dogs

2015 ◽  
Vol 143 (15) ◽  
pp. 3277-3291 ◽  
Author(s):  
A. F. SMITH ◽  
M. ROCK ◽  
N. NEUMANN ◽  
A. MASSOLO
Keyword(s):  
Risk Factors ◽  
Urban Areas ◽  
Immunofluorescence Assay ◽  
Urban Park ◽  
Direct Immunofluorescence ◽  
Park Use ◽  
Faecal Samples ◽  
Demographic Risk ◽  
Demographic Risk Factors ◽  
Direct Immunofluorescence Assay

SUMMARYGiardiaspp. is a common gastrointestinal (GI) parasite of multiple host species, including dogs and humans, with the potential for zoonotic transmission. The risk of GI parasitism in dogs (includingGiardiaspp.) may increase with park use in urban areas. This study aimed to (1) determine whether park attendance is a risk factor forGiardiaspp. infection in dogs and (2) characterize the behavioural and demographic risk factors forGiardiaspp. infection in park-attending and non-park-attending dogs. From August to September 2012, a total of 1293 dog owners completed a survey and 860 corresponding dog faecal samples were collected. Dog faeces were screened forGiardiaspp. using a direct immunofluorescence assay and associations assessed among behaviours, demographics, andGiardiaspp. infection. Main results included off-leash and swimming frequencies within parks as significantly positively associated withGiardiaspp. infection in dogs. Dog-owner age was negatively associated with off-leash and swimming frequencies in parks. The results suggest some recreational behaviours in parks and certain demographics are risk factors for parasitism in pet dogs.

scholarly journals A Sensitive, Specific, and Cost-Effective Multiplex Reverse Transcriptase-PCR Assay for the Detection of Seven Common Respiratory Viruses in Respiratory Samples

2004 ◽  
Vol 6 (2) ◽  
pp. 125-131 ◽  
Author(s):  
Melanie W. Syrmis ◽  
David M. Whiley ◽  
Marion Thomas ◽  
Ian M. Mackay ◽  
Jeanette Williamson ◽  
...  
Keyword(s):  
Reverse Transcriptase ◽  
Cost Effective ◽  
Respiratory Viruses ◽  
Pcr Assay ◽  
Reverse Transcriptase Pcr
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