EMBRYO  PERFORMANCE  OF  SIMMENTAL  DONOR  COWS  AND  RESULTS  OF EMBRYO  MORPHOLOGICAL  EVALUATION

doi:10.52578/2305-9397-2021-1-1-49-56

EMBRYO PERFORMANCE OF SIMMENTAL DONOR COWS AND RESULTS OF EMBRYO MORPHOLOGICAL EVALUATION

Ġylym ža̋ne bìlìm ◽

10.52578/2305-9397-2021-1-1-49-56 ◽

2021 ◽

Vol 1 (1) ◽

pp. 49-56

Keyword(s):

Morphological Parameters ◽

Morphological Evaluation ◽

Simmental Cattle ◽

Donor Cows

This article discusses the importance of embryo transplantation for cattle and obtaining an embryo by creating a superovulation for Simmental cattle, evaluating embryos by morphological parameters and determining their suitability for transplantation, as well as methods for extracting, evaluating the quality and transplantation of embryos. According to the results of insemination, 113 embryos were obtained from 9 donors. Of these, 76.7 % are suitable, the remaining 23.3 % are not suitable for transplantation, and an average of 12.5 embryos can be obtained from a single cow. From 6 donors, 71 embryos were obtained by insemination with bisexual sperm, 65 embryos are suitable, 6 embryos are not suitable for transplantation, in the middle 11.8 embryos from a cow. From 3 donors, 42 embryos were obtained by insemination with sexed seed, 26 suitable, 16 unsuitable for transplantation, and an average of 14 embryos from a cow

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Does Conventional Morphological Evaluation Still Play a Role in Predicting Blastocyst Formation?

10.21203/rs.3.rs-1161968/v1 ◽

2021 ◽

Author(s):

Xiaoming Jiang ◽

Jiali Cai ◽

Lanlan Liu ◽

Zhenfang Liu ◽

Wenjie Wang ◽

...

Keyword(s):

Patient Characteristics ◽

Time Lapse ◽

Cell Number ◽

Blastocyst Transfer ◽

Embryo Morphology ◽

Morphological Parameters ◽

Lasso Regression ◽

Blastocyst Formation ◽

Morphological Evaluation ◽

Validation Set

Abstract BackgroundAdvanced models including time-lapse imaging and artificial intelligence technologies have been used to predict blastocyst formation. However, the conventional morphological evaluation of embryos is still widely used. The purpose of the present study was to evaluate the predictive power of conventional morphological evaluation regarding blastocyst formation.MethodsRetrospective evaluation of data from 15613 patients receiving blastocyst culture from January 2013 through December 2020 in our institution were reviewed. Generalized estimating equations (GEE) were used to establish the morphology-based model. To estimate whether including more features regarding patient characteristics and cycle parameters improve the predicting power, we also establish models including 27 more features with either LASSO regression or XGbosst. The predicted number of blastocyst were associated with the observed number of the blastocyst and were used to predict the blastocyst transfer cancellation either in fresh or frozen cycles. ResultsBased on early cleavage and routine observed morphological parameters (cell number, fragmentation, and symmetry), the GEE model predicted blastocyst formation with an AUC of 0.779(95%CI: 0.77-0.787) and an accuracy of 74.7%(95%CI: 73.9%-75.5%) in the validation set. LASSO regression model and XGboost model based on the combination of cycle characteristics and embryo morphology yielded similar predicting power with AUCs of 0.78(95%CI: 0.771-0.789) and 0.754(95%CI: 0.745-0.763), respectively. For per-cycle blastocyst yield, the predicted number of blastocysts using morphological parameters alone strongly correlated with observed blastocyst number (r=0.897, P<0.0001) and predicted blastocyst transfer cancel with an AUC of 0.926((95%CI: 0.911-0.94). ConclusionThe data suggested that routine morphology observation remained a feasible tool to support an informed decision regarding the day of transfer. However, models based on the combination of cycle characteristics and embryo morphology do not increase the predicting power significantly.

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Hexachlorobenzene toxicity in the rat ovary: II. Ultrastructure induced by medium (10 mg/kg) dose exposure

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010012374x ◽

1992 ◽

Vol 50 (1) ◽

pp. 668-669

Author(s):

Amreek Singh ◽

Warren G. Foster ◽

Anna Dykeman ◽

David C. Villeneuve

Keyword(s):

Germ Cells ◽

Primordial Germ Cells ◽

Surface Epithelium ◽

Morphological Parameters ◽

Comprehensive Investigation ◽

Ovary Surface Epithelium ◽

Rat Ovary ◽

High Doses

Hexachlorobenzene (HCB) is a known toxicant that is found in the environment as a by-product during manufacture of certain pesticides. This chlorinated chemical has been isolated from many tissues including ovary. When administered in high doses, HCB causes degeneration of primordial germ cells and ovary surface epithelium in sub-human primates. A purpose of this experiment was to determine a no-effect dose of the chemical on the rat ovary. The study is part of a comprehensive investigation on the effects of the compound on the biochemical, hematological, and morphological parameters in the monkey and rat.

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Distribution of VLA-5 and VLA-4 fibronectin receptors in human monocytes demonstrated by immunofluorescence, immunocytochemistry, and autoradiography

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100147375 ◽

1993 ◽

Vol 51 ◽

pp. 306-307

Author(s):

Robert Williams ◽

Che-Hung Lee ◽

Sara E. Quella ◽

David M. Harlan ◽

Yuan-Hsu Kang

Keyword(s):

Present Report ◽

Matrix Proteins ◽

Extracellular Matrices ◽

Human Monocytes ◽

Integrin Receptors ◽

Morphological Evaluation ◽

The Matrix ◽

Specific Receptors ◽

Cytokine Stimulation ◽

Monocyte Adherence

Monocyte adherence to endothelial or extracellular matrices plays an important role in triggering monocyte activation in extravascular sites of infection, chronic inflammatory disorders, and tissue damage. Migration of monocytes in the tissues involves the response to a chemoattractant and movement by a series of attachments and detachments to the extracellular matrices which are regulated by expression and distribution of specific receptors for the matrix proteins such as fibronectin (FN). The VSAs (very late antigens or beta integrins), a subfamily of the transmembrane heterodimeric integrin receptors, have been thought to play a major role in monocyte adherence to the extracellular matrices and cells. In this subfamily, VLA-5 and VLA-4 are believed to be the most essential integrins mediating monocyte adherence to FN. In the present report, we have established and compared different procedures for morphological evaluation of the expression and distribution of the FN receptors on human monocytes in order to investigate their response to endotoxin or cytokine stimulation.

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