Simultaneous determination of Sulphadoxine and Pyrimethamine by taking Tolterodine as an internal standard in Pharmaceutical dosage form by RP-HPLC

2021 ◽  
pp. 145-150
Author(s):  
Sushil D. Patil ◽  
Pravin B. Shelke ◽  
Priti Aher ◽  
Maswood Ahmed Hafizur Rahman
Keyword(s):  
Simultaneous Determination ◽  
Dosage Form ◽  
Internal Standard ◽  
Hplc Method ◽  
Control Analysis ◽  
Pharmaceutical Dosage Form ◽  
Quality Control Analysis ◽  
Rp Hplc ◽  
Sulphadoxine Pyrimethamine

A simple, rapid, economic, sensitive and precise HPLC method has been developed for the simultaneous determination of Sulphadoxine and Pyrimethamine in pharmaceutical dosage form by taking Tolterodine as an internal standard. The method was carried out using Phenomenex C18 (4.6ID × 250mm; 5µm) column and mobile phase comprised of methanol and Phosphate Buffer in proportion of ratio 60:40 v/v. The flow rate was 1.0mL/min and detection was carried out at 276nm. The retention time of Sulphadoxine, Pyrimethamine and Tolterodine were found to be 2.967, 4.058 and 6.908 respectively. Linearity of Sulphadoxine and Pyrimethamine in the range of 2 to 12μg/mL and 4 to 24μg/mL respectively. The % recoveries of Sulphadoxine and Pyrimethamine were found to be in between 99.93% to 99. 96 % respectively. The proposed method is suitable for the routine quality control analysis for simultaneous determination of Sulphadoxine and Pyrimethamine was in bulk and pharmaceutical dosage form.

scholarly journals Simultaneous Determination of Lamivudine, Zidovudine and Abacavir in Tablet Dosage Forms by RP HPLC Method

2010 ◽  
Vol 7 (1) ◽  
pp. 180-184 ◽  
Author(s):  
D. Anantha Kumar ◽  
G. Srinivasa Rao ◽  
J. V. L. N. Seshagiri Rao
Keyword(s):  
Simultaneous Determination ◽  
Internal Standard ◽  
Dosage Forms ◽  
Hplc Method ◽  
Control Analysis ◽  
Potassium Dihydrogen ◽  
Quality Control Analysis ◽  
Rp Hplc ◽  
Tablet Dosage

A simple, accurate and reproducible RP-HPLC method has been developed for the simultaneous determination of lamivudine, zidovudine and abacavir in tablet dosage forms. Chromatography was carried out on a HiQ Sil C 18 V column using a mobile phase consisting of 0.01 M potassium dihydrogenortho-phosphate (pH 3.0) and methanol (55:45 v/v) at a flow rate of 0.8 mL/min. The detection was made at 272 nm and stavudine was used as the internal standard for this study. The retention times for lamivudine, abacavir and zidovudine were found to be 3.8, 6.3, 8.1 min. respectively. The calibration curves were linear over the range 5-250 μg/mL for both zidovudine and abacavir and 5-140 μg/mL for lamivudine. The proposed method was validated as per ICH and USP guidelines and it was found suitable for the routine quality control analysis of the drugs in tablet dosage forms.

scholarly journals RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF LOSARTAN AND CHLORTHALIDONE IN PHARMACEUTICAL DOSAGE FORM

2015 ◽  
Vol 6 (7) ◽  
pp. 453-457 ◽  
Author(s):  
Aneesh T P ◽  
Renju Radhakrishnan ◽  
Aravind P M ◽  
Anuja Sasidharan ◽  
Manisha Choyal
Keyword(s):  
Simultaneous Determination ◽  
Dosage Form ◽  
Hplc Method ◽  
Pharmaceutical Dosage Form ◽  
Rp Hplc

Comparison of Chemometric assisted UV Spectrophotometric and RP-HPLC Method for the simultaneous determination of Ofloxacin and Tinidazole in their Combined dosage form

2021 ◽  
pp. 5713-5718
Author(s):  
Santosh V. Gandhi ◽  
Deepak Patil ◽  
Atul A. Baravkar
Keyword(s):  
Simultaneous Determination ◽  
Dosage Form ◽  
Hplc Method ◽  
Partial Least Square ◽  
Least Square ◽  
Partial Least Square Regression ◽  
Simultaneous Estimation ◽  
Pharmaceutical Dosage Form ◽  
Rp Hplc

In present work, chemometric-assisted UV spectrophotometric methods as well as RP-HPLC method were developed for the simultaneous estimation of Ofloxacin and Tinidazole in their combined pharmaceutical dosage form. The two chemometric methods i.e. principle component regression (PCR) and partial least square regression (PLS) were successfully applied to quantify each drug in mixture using UV absorption spectra in range of 280 to 320nm at ∆λ of 0.5nm. Chemometric model development was done using 24 binary mixture solutions and 12 solutions were used for validation of model. The chemometric-assisted analysis does not require any prior separation step. In addition, RP-HPLC method was also developed using THERMOSIL C18 column with a mobile phase consisting ofAcetonitrile: Phosphate Buffer (85:15% v/v), flow rate of 1 ml/min and quantification was achieved using UV detector at 300 nm. The methods were successfully applied for the simultaneous determination of these drugs in synthetic mixture. The results obtained for analysis by PCR and PLS methods were compared with RP-HPLC method and a good agreement was found.

Simultaneous Determination of Chloroquine and Pyrimethamine with Cetirizine in an Active Form and Human Serum by RP-HPLC

2021 ◽  
Author(s):  
Hina Shamshad ◽  
Ali Sayqal ◽  
Jahan Zeb ◽  
Agha Zeeshan Mirza
Keyword(s):  
Human Serum ◽  
Simultaneous Determination ◽  
Active Form ◽  
Internal Standard ◽  
Hplc Method ◽  
Serum Samples ◽  
Rp Hplc ◽  
Cetirizine Hydrochloride ◽  
Bulk Drug

Abstract A simple, accurate and precise RP-HPLC method was developed for the simultaneous determination of chloroquine, pyrimethamine and cetirizine hydrochloride concentrations in bulk drug and human serum. The assay was performed using a mobile phase of methanol: water (70:30) at pH of 2.8 ± 0.05 on the Purospher C-18 column with UV detection at 230 nm and rosuvastatin used as an internal standard. The retention times observed for chloroquine, pyrimethamine and cetirizine hydrochloride were 3.5, 2.5 and 5.5 minutes, respectively. The method was found to be specific for the assayed drugs showing a linear response in the concentration range of 1–100 μg mL−1 with coefficients of determination values of (r = 0.999). The method was developed and validated according to ICH guidelines. The method was used to monitor the serum samples and was found to be sensitive for therapeutic purposes, showing the potential to be a useful tool for routine analysis in laboratories.

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