Stability Indicating Simultaneous Estimation of Cetirizine Hydrochloride and Phenylephrine Hydrochloride in Combined tablet formulation by using HPTLC- Densitometric method

The new simple, precise, rapid, selective, high performance thin layer chromatography method has been developed for simultaneous estimation of cetirizine hydrochloride and phenylephrine hydrochloride in its combined solid dosage form. The HPTLC method was performed on precoated silica gel G60 F254 plates with, Toluene: Methanol: Ammonia (8: 2: 1, v/v/v) as optimum mobile phase. The plates were developed in 6.00× 0.30mm at room temperature. The developed plates were scanned and quantified at their absorption at 224 nm for CET and PHE respectively. The drugs were satisfactorily resolved with Rf = 0.36± 0.001 for CET and 0.65±0.004 for PHE. Calibration plot was linear between 100-600 ng/ spot for CET and 200-1200 ng/ spot for PHE. This HPTLC method is sensitive, economical, less time consuming than other chromatographic procedures. It can become important tool for analysis of combined solid dosage from. In addition to this results acid exposure, base exposure, oxidative exposure, neutral exposure, photolysis and dry heat exposure is carried out.

Alteration of Serum Liver Enzymes Level Caused by Cetirizine Hydrochloride in Exercise Performers

Background: Players regularly use cetirizine HCL for the prevention and treatment of various symptoms of allergy while playing in unfamiliar environments. Objective: This research study was carried out to examine the effect of cetirizine HCL on liver function (ALT, ALP and AST) among the players. Methods: A descriptive study was carried out at Gomal University, Dera Ismail Khan from January 2019 to June 2019.Twenty male subjects 10 players using cetirizine HCL (one tablet of 10mg/day) as experimental group and 10 players not using cetirizine HCL as control group was selected voluntarily as participants of the study. Five ml blood was taken from all subjects after three hours of administration of cetirizine HCL for the assessment of ALT, ALP and AST. The results obtained through liver functions test (LFTs) were analyzed through a statistical package for social sciences (SPSS, Version 24). Results: Significant difference was found in both groups (CG and EXG) in term of ALT (P = 000, 0.05>.000). There was significant difference between in both groups (CG and EXG) in term of ALP (P = 000, 0.05>.000). A significant difference was found between in both groups (CG and EXG) in term of AST (P = .001, 0.05>.000). Conclusion: on the basis of finding, the researcher concluded that Cetirizine HCL (Tablet 10mg) produced a significant rise in liver enzymes and hence disturbing the normal functioning of liver.

Simultaneous Determination of Chloroquine and Pyrimethamine with Cetirizine in an Active Form and Human Serum by RP-HPLC

A simple, accurate and precise RP-HPLC method was developed for the simultaneous determination of chloroquine, pyrimethamine and cetirizine hydrochloride concentrations in bulk drug and human serum. The assay was performed using a mobile phase of methanol: water (70:30) at pH of 2.8 ± 0.05 on the Purospher C-18 column with UV detection at 230 nm and rosuvastatin used as an internal standard. The retention times observed for chloroquine, pyrimethamine and cetirizine hydrochloride were 3.5, 2.5 and 5.5 minutes, respectively. The method was found to be specific for the assayed drugs showing a linear response in the concentration range of 1–100 μg mL−1 with coefficients of determination values of (r = 0.999). The method was developed and validated according to ICH guidelines. The method was used to monitor the serum samples and was found to be sensitive for therapeutic purposes, showing the potential to be a useful tool for routine analysis in laboratories.