scholarly journals Detection of Bovine Herpesvirus 1 from Semen by Real-time PCR to Prevent the Spread of Infectious Bovine Rhinotracheitis Infection

2021 ◽  
Vol 11 (4) ◽  
pp. 709-712
Author(s):  
Tri Untari ◽  
Yuli Purwandari Kristianingrum ◽  
Asmarani Kusumawati
Keyword(s):  
Real Time ◽  
Artificial Insemination ◽  
Real Time Pcr ◽  
Disease Transmission ◽  
Bovine Herpesvirus ◽  
Infectious Bovine Rhinotracheitis ◽  
Bovine Herpesvirus 1 ◽  
The Real ◽  
Herpesvirus 1

Infectious Bovine Rhinotracheitis (IBR) can be transmitted by livestock seeds and semen, through the process of artificial insemination. Therefore, it is necessary to detect the presence of the Bovine Herpesvirus 1 (BHV1) in semen through artificial insemination and breeding centers of Indonesia. The current study aimed to detect the presence of the virus in semen as a source of IBR disease transmission in Indonesia. A total of 27 semen samples from artificial insemination and breeding centers (Sembawa, Lembang, Ungaran, and Sleman) in Indonesia have been examined and identified using the real-time PCR (qPCR) technique. The result showed that all samples were negative to BHV1. This indicated that semen from Ungaran, Sembawa, Sleman, and Lembang was safe to be used as a semen source for artificial insemination.

EvaGreen-based Multiplex Real-time PCR Assay for Rapid Differentiation of Wild-Type and Glycoprotein E-Deleted Bovine Herpesvirus-1 Strains

2017 ◽  
Vol 28 (4) ◽  
pp. 248-252 ◽  
Author(s):  
Sachin S. Pawar ◽  
Chetan D. Meshram ◽  
Niraj K. Singh ◽  
Mohini Saini ◽  
B. P. Mishra ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Bovine Herpesvirus ◽  
Wild Type ◽  
Pcr Assay ◽  
Bovine Herpesvirus 1 ◽  
Glycoprotein E ◽  
Herpesvirus 1

scholarly journals Evaluation of a specialized filter-paper matrix for transportation of extended bovine semen to screen for bovine herpesvirus-1 by real-time PCR

2018 ◽  
Vol 257 ◽  
pp. 1-6 ◽  
Author(s):  
Laxmi Narayan Sarangi ◽  
Thodangala Naveena ◽  
Samir Kumar Rana ◽  
Kota Sri Naga Leela Surendra ◽  
Rachamreddy Venkata Chandrasekhar Reddy ◽  
...  
Keyword(s):  
Real Time ◽  
Filter Paper ◽  
Real Time Pcr ◽  
Bovine Herpesvirus ◽  
Bovine Herpesvirus 1 ◽  
Bovine Semen ◽  
Herpesvirus 1

An international inter-laboratory ring trial to evaluate a real-time PCR assay for the detection of bovine herpesvirus 1 in extended bovine semen

2008 ◽  
Vol 126 (1-3) ◽  
pp. 11-19 ◽  
Author(s):  
Jianning Wang ◽  
Joseph O’Keefe ◽  
Della Orr ◽  
Leo Loth ◽  
Malcolm Banks ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Bovine Herpesvirus ◽  
Pcr Assay ◽  
Bovine Herpesvirus 1 ◽  
Bovine Semen ◽  
Ring Trial ◽  
Herpesvirus 1

Development of a SYBR Green I based duplex real-time PCR for detection of bovine herpesvirus-1 in semen

2014 ◽  
Vol 208 ◽  
pp. 6-10 ◽  
Author(s):  
Sachin S. Pawar ◽  
Chetan D. Meshram ◽  
Niraj K. Singh ◽  
Mohini Saini ◽  
B.P. Mishra ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Bovine Herpesvirus ◽  
Sybr Green ◽  
Sybr Green I ◽  
Bovine Herpesvirus 1 ◽  
Herpesvirus 1

Validation of a real-time PCR assay for the detection of bovine herpesvirus 1 in bovine semen

2007 ◽  
Vol 144 (1-2) ◽  
pp. 103-108 ◽  
Author(s):  
Jianning Wang ◽  
Joseph O’Keefe ◽  
Della Orr ◽  
Leo Loth ◽  
Malcolm Banks ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Bovine Herpesvirus ◽  
Pcr Assay ◽  
Bovine Herpesvirus 1 ◽  
Bovine Semen ◽  
Herpesvirus 1

scholarly journals First Report on the Detection and Molecular Characterization of Bovine Herpesvirus 1 from a Clinical case of Infectious Bovine Rhinotracheitis in Pakistan

2021 ◽  
Vol 41 (01) ◽  
pp. 160-162
Author(s):  
Aayesha Riaz
Keyword(s):  
Molecular Characterization ◽  
Nested Pcr ◽  
Bovine Herpesvirus ◽  
Nasal Discharge ◽  
Infectious Bovine Rhinotracheitis ◽  
Bovine Herpesvirus 1 ◽  
Nasal Swabs ◽  
Herpesvirus 1 ◽  
First Time

Bovine herpesvirus 1 (BHV-1) is a noteworthy reason for many Cattle/Buffalo diseases. Infectious bovine rhinotracheitis (IBR) is one of the diseases which are caused by the BHV-1. In the present study a cow which was suspected of IBR was examined. The animal was suffering from fever and respiratory distress along with rhinitis (red nose), in appetence, and dyspnea. The nasal mucosa and muzzle were distinctly inflamed, with nasal discharge. DNA from blood samples and nasal swabs were subjected to nested PCR using glycoprotein B gene (gB) Primers. The samples were found positive for BHV-1 gB gene. Sequencing and phylogenetic analysis revealed close similarities with other BHV-1 gB gene sequences. The accession numbers assigned to this pioneer sequences in GenBank are MT449510 (BHV-1-Pak 1) and MT449511 (BHV-1-Pak 2). In this study, we reported for the first time the detection of DNA of BHV-1 through nested PCR assay and molecular characterization of BHV-1 gB gene in Pakistan. This study will be useful in further diagnoses of BHV-1 in Pakistan and in development of BHV-1 vaccine to reduce economical losses due to IBR

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