scholarly journals Immunogenicity of Recombinant DNA Vaccine Encoding Non-Structural Protein-1 Dengue Virus Serotype-2 in Balb/c Mice

2021 ◽  
Vol 15 (2) ◽  
pp. 69-75
Author(s):  
FITHRIYAH SJATHA ◽  
◽  
ELITHA SUNDARI PULUNGAN ◽  
TJAHJANI MIRAWATI SUDIRO ◽  
◽  
...  
Keyword(s):  
Dengue Virus ◽  
Dna Vaccine ◽  
Recombinant Dna ◽  
Structural Protein ◽  
Virus Serotype ◽  
Dengue Virus Serotype 2

scholarly journals Virus-Like Particle Secretion and Genotype-Dependent Immunogenicity of Dengue Virus Serotype 2 DNA Vaccine

2014 ◽  
Vol 88 (18) ◽  
pp. 10813-10830 ◽  
Author(s):  
J. U. Galula ◽  
W.-F. Shen ◽  
S.-T. Chuang ◽  
G.-J. J. Chang ◽  
D.-Y. Chao
Keyword(s):  
Dengue Virus ◽  
Dna Vaccine ◽  
Virus Like Particle ◽  
Virus Serotype ◽  
Dengue Virus Serotype 2

scholarly journals Immunogenicity of Recombinant DNA Vaccine Encoding Non-Structural Protein-1 Dengue Virus Serotype-2 in Balb/c Mice

2021 ◽  
Vol 15 (2) ◽  
pp. 4
Author(s):  
Elitha Pulungan
Keyword(s):  
Immune Response ◽  
Dengue Virus ◽  
Dna Vaccine ◽  
Humoral Immune Response ◽  
Structural Proteins ◽  
Ns1 Protein ◽  
Structural Protein ◽  
Humoral Immune ◽  
Virus Serotype ◽  
Positive Strand Rna

Background: Dengue Hemorrhagic Fever (DHF) is an infectious disease caused by the dengue virus (DENV) which spread widely in tropical and subtropical regions of the world. DENV is a single-positive strand RNA virus with a genome size of ± 11kb which encodes three structural proteins, seven non-structural proteins, and two untranslated regions (UTR). The non-structural protein-1 (NS1) of DENV is known to have important role in dengue pathogenesis also promising to be developed as dengue vaccine. Lately, novel vaccine approach by DNA immunization have given new perspective for a safe, stable, and immunogenic vaccine platform. Previously, we have successfully construct DNA vaccine encoding NS1 protein of DENV2 (pUNS1) which express recombinant NS1 protein in-vitro. Thus, in this current study the ability of pUNS1 to induce humoral immune response will be further analyzed by in mice immunization. Methods: Sixteen BALB/c mice aged of 4 weeks were immunized 3 times with 100 µg of pUNS1 or pUMVC4a on 2 week time interval. Blood sampling was carried out just before immunization and termination was done 2 week after last immunization. Titer from individual mice sera against DENV-2 were measure with in-house ELISA. Results: IgG against NS1 protein of DENV2 titer from mice group immunized with recombinant pUNS1 shown high ELISA absorbancies, 5 times higher than pUMVC4a group. This result suggest the ability of pUNS1 to induce humoral immune response against NS1 DENV-2 in-vivo. Conclusion: Recombinant pUNS1 can induce humoral immune response in mice.

scholarly journals Evaluation of protection induced by a dengue virus serotype 2 envelope domain III protein scaffold/DNA vaccine in non-human primates

Vaccine ◽  
2016 ◽  
Vol 34 (30) ◽  
pp. 3500-3507 ◽  
Author(s):  
Sean P. McBurney ◽  
Justine E. Sunshine ◽  
Sarah Gabriel ◽  
Jeremy P. Huynh ◽  
William F. Sutton ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Dna Vaccine ◽  
Protein Scaffold ◽  
Virus Serotype ◽  
Domain Iii ◽  
Dengue Virus Serotype 2

scholarly journals Molecular characterization of structural protein genes of dengue virus serotype 1 epidemic in Yunnan, Southwest China, in 2018

2021 ◽  
Vol 166 (3) ◽  
pp. 863-870
Author(s):  
Qingping Lan ◽  
Yun Shu ◽  
Linhao Li ◽  
Xiyun Shan ◽  
Dehong Ma ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Molecular Characterization ◽  
Southwest China ◽  
Structural Protein ◽  
Virus Serotype ◽  
Serotype 1 ◽  
Dengue Virus Serotype 1

Dengue Virus Serotype-2 Interferes with the Formation of Neutrophil Extracellular Traps

Intervirology ◽  
2015 ◽  
Vol 58 (4) ◽  
pp. 250-259 ◽  
Author(s):  
Maria Maximina B. Moreno-Altamirano ◽  
Oscar Rodríguez-Espinosa ◽  
Oscar Rojas-Espinosa ◽  
Bernardo Pliego-Rivero ◽  
Francisco J. Sánchez-García
Keyword(s):  
Dengue Virus ◽  
Neutrophil Extracellular Traps ◽  
Extracellular Traps ◽  
Virus Serotype ◽  
Dengue Virus Serotype 2

Impact of Dengue Virus Serotype 2 Strain Diversity on Serological Immune Responses to Dengue

2018 ◽  
Vol 4 (12) ◽  
pp. 1705-1717 ◽  
Author(s):  
Sarah L. Keasey ◽  
Jessica L. Smith ◽  
Stefan Fernandez ◽  
Anna P. Durbin ◽  
Bryan M. Zhao ◽  
...  
Keyword(s):  
Immune Responses ◽  
Dengue Virus ◽  
Strain Diversity ◽  
Virus Serotype ◽  
Dengue Virus Serotype 2

scholarly journals Expression of Recombinant Non Structural 1 Protein of Dengue Virus Serotype-2 in Mammalian Cell Line

2019 ◽  
Vol 13 (1) ◽  
pp. 36-42 ◽  
Author(s):  
FITHRIYAH SJATHA ◽  
◽  
OKTIVIA CHANDRA MUSTIKA ◽  
ANGKY BUDIANTI ◽  
TJAHJANI MIRAWATI SUDIRO ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Cell Line ◽  
Mammalian Cell ◽  
Mammalian Cell Line ◽  
Virus Serotype ◽  
Dengue Virus Serotype 2

scholarly journals Subversion of immunoproteasome subunit expression in dengue virus serotype 2-infected HepG2 cells

2017 ◽  
Vol 50 (1) ◽  
pp. 99-103
Author(s):  
Chye Sheng Gan ◽  
Pei Jean Lim ◽  
Muhammad Fazril Mohamad Razif ◽  
Rohana Yusof ◽  
Shatrah Othman
Keyword(s):  
Dengue Virus ◽  
Hepg2 Cells ◽  
Virus Serotype ◽  
Subunit Expression ◽  
Dengue Virus Serotype 2

Peptides P4 and P7 derived from E protein inhibit entry of dengue virus serotype 2 via interacting with β3 integrin

2018 ◽  
Vol 155 ◽  
pp. 20-27 ◽  
Author(s):  
Xiaoyun Cui ◽  
Yanhua Wu ◽  
Dongying Fan ◽  
Na Gao ◽  
Ying Ming ◽  
...  
Keyword(s):  
Dengue Virus ◽  
E Protein ◽  
Virus Serotype ◽  
Β3 Integrin ◽  
Dengue Virus Serotype 2
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