scholarly journals Establishment of Mycorrhizas of Four Different Species of Arbuscular Mycorrhizal Fungi Observed Using Petri-Dish Microcosm

2010 ◽  
Vol 4 (1) ◽  
pp. 27-32
Author(s):  
IRDIKA MANSUR ◽  
JOHN DODD ◽  
PETER JEFFRIES
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Petri Dish

A “paper bridge” system to improve in-vitro propagation of arbuscular mycorrhizal fungi

Botany ◽  
2010 ◽  
Vol 88 (6) ◽  
pp. 617-620 ◽  
Author(s):  
Yolande Dalpé ◽  
Sylvie Seguin
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Petri Dish ◽  
Efficient System ◽  
Arbuscular Mycorrhizal Fungal ◽  
Slow Growing ◽  
Paper Bridge ◽  
Bridge System

The in-vitro culture of arbuscular mycorrhizal fungi on excised roots, especially when performed on bi-compartmented Petri dishes, has proven to be an efficient system for the production of root-free fungal material. However, even after the contact between fungal hyphae and the excised roots in the proximal root compartment has occurred, up to several weeks may be required for the fungal runner hyphae to cross the median Petri dish wall and reach the distal fungal compartment. This delay is particularly long for the cultivation of slow-growing strains that usually colonize the substrate less aggressively. The delay is due to the difficulty the runner hyphae have in crossing the median Petri dish wall that separates compartments. To facilitate the passage of the fungus across the median wall, a “paper bridge” system has been devised and tested with a number of arbuscular mycorrhizal fungal strains. This method substantially accelerated fungal propagation and simplified the manipulations necessary. The proposed paper-bridge system is described and its advantages discussed.

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