scholarly journals Application of Phenol Pretreatment for the Isolation of Rare Actinomycetes from Indonesian Soil

2012 ◽  
Vol 6 (1) ◽  
pp. 42-47 ◽  
Author(s):  
YUDHIE ISTIANTO ◽  
RADEN SETYO ADJI KOESOEMOWIDODO ◽  
YOSHIO WATANABE ◽  
HARDANING PRANAMUDA ◽  
BAMBANG MARWOTO
Keyword(s):  
Rare Actinomycetes

Bioactive Metabolites from Rare Actinomycetes

2014 ◽  
pp. 419-512 ◽  
Author(s):  
Kavita Tiwari ◽  
Rajinder K. Gupta
Keyword(s):  
Bioactive Metabolites ◽  
Rare Actinomycetes

scholarly journals Polyketide Synthase and Nonribosomal Peptide Synthetase Gene Clusters in Type Strains of the Genus Phytohabitans

Life ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 257
Author(s):  
Hisayuki Komaki ◽  
Tomohiko Tamura
Keyword(s):  
Secondary Metabolites ◽  
Polyketide Synthase ◽  
Gene Clusters ◽  
Nonribosomal Peptide Synthetase ◽  
Nrps Gene ◽  
Nonribosomal Peptide ◽  
Rare Actinomycetes ◽  
Whole Genomes ◽  
Peptide Synthetase ◽  
Established Genus

(1) Background: Phytohabitans is a recently established genus belonging to rare actinomycetes. It has been unclear if its members have the capacity to synthesize diverse secondary metabolites. Polyketide and nonribosomal peptide compounds are major secondary metabolites in actinomycetes and expected as a potential source for novel pharmaceuticals. (2) Methods: Whole genomes of Phytohabitans flavus NBRC 107702T, Phytohabitans rumicis NBRC 108638T, Phytohabitans houttuyneae NBRC 108639T, and Phytohabitans suffuscus NBRC 105367T were sequenced by PacBio. Polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS) gene clusters were bioinformatically analyzed in the genome sequences. (3) Results: These four strains harbored 10, 14, 18 and 14 PKS and NRPS gene clusters, respectively. Most of the gene clusters were annotated to synthesis unknown chemistries. (4) Conclusions: Members of the genus Phytohabitans are a possible source for novel and diverse polyketides and nonribosomal peptides.

scholarly journals Studies on the Isolation and Distribution of Rare Actinomycetes in Soil

2008 ◽  
Vol 22 (1) ◽  
pp. 12-19 ◽  
Author(s):  
Masayuki Hayakawa
Keyword(s):  
Rare Actinomycetes

scholarly journals Control of chitin and N-acetylglucosamine utilization in Saccharopolyspora erythraea

Microbiology ◽  
2014 ◽  
Vol 160 (9) ◽  
pp. 1914-1928 ◽  
Author(s):  
Chengheng Liao ◽  
Sébastien Rigali ◽  
Cuauhtemoc Licona Cassani ◽  
Esteban Marcellin ◽  
Lars Keld Nielsen ◽  
...  
Keyword(s):  
Transcriptional Control ◽  
Streptomyces Coelicolor ◽  
Saccharopolyspora Erythraea ◽  
Phosphotransferase System ◽  
Nutrient Source ◽  
Intracellular Degradation ◽  
Rare Actinomycetes ◽  
Genes Encoding ◽  
Or Genes ◽  
Model Strain

Chitin degradation and subsequent N-acetylglucosamine (GlcNAc) catabolism is thought to be a common trait of a large majority of actinomycetes. Utilization of aminosugars had been poorly investigated outside the model strain Streptomyces coelicolor A3(2), and we examined here the genetic setting of the erythromycin producer Saccharopolyspora erythraea for GlcNAc and chitin utilization, as well as the transcriptional control thereof. Sacch. erythraea efficiently utilize GlcNAc most likely via the phosphotransferase system (PTSGlcNAc); however, this strain is not able to grow when chitin or N,N′-diacetylchitobiose [(GlcNAc)2] is the sole nutrient source, despite a predicted extensive chitinolytic system (chi genes). The inability of Sacch. erythraea to utilize chitin and (GlcNAc)2 is probably because of the loss of genes encoding the DasABC transporter for (GlcNAc)2 import, and genes for intracellular degradation of (GlcNAc)2 by β-N-acetylglucosaminidases. Transcription analyses revealed that in Sacch. erythraea all putative chi and GlcNAc utilization genes are repressed by DasR, whereas in Strep. coelicolor DasR displayed either activating or repressing functions whether it targets genes involved in the polymer degradation or genes for GlcNAc dimer and monomer utilization, respectively. A transcriptomic analysis further showed that GlcNAc not only activates the transcription of GlcNAc catabolism genes but also activates chi gene expression, as opposed to the previously reported GlcNAc-mediated catabolite repression in Strep. coelicolor. Finally, synteny exploration revealed an identical genetic background for chitin utilization in other rare actinomycetes, which suggests that screening procedures that used only the chitin-based protocol for selective isolation of antibiotic-producing actinomycetes could have missed the isolation of many industrially promising strains.

Isolation and Identification of Potential Antibiotic Producing Rare Actinomycetes from Rhizospheric Soils

2016 ◽  
Vol 56 (1-2) ◽  
pp. 31-41 ◽  
Author(s):  
Mobolaji Felicia Adegboye ◽  
Olubukola Oluranti Babalola
Keyword(s):  
Isolation And Identification ◽  
Rare Actinomycetes

Diversity and isolation of rare actinomycetes: an overview

2012 ◽  
Vol 39 (3) ◽  
pp. 256-294 ◽  
Author(s):  
Kavita Tiwari ◽  
Rajinder K. Gupta
Keyword(s):  
Rare Actinomycetes
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