scholarly journals An efficient plant regeneration through embryogenic callus formation and direct somatic embryogenesis via immature embryo culture in Ipomoea purpurea and I. tricolor

2014 ◽  
Vol 31 (2) ◽  
pp. 179-183 ◽  
Author(s):  
Hiroki Ishikuro ◽  
Eiji Nitasaka ◽  
Motoyasu Otani
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Embryo Culture ◽  
Embryogenic Callus ◽  
Callus Formation ◽  
Immature Embryo ◽  
Direct Somatic Embryogenesis ◽  
Ipomoea Purpurea ◽  
Immature Embryo Culture ◽  
Embryogenic Callus Formation

scholarly journals Plant regeneration from immature embryo culture of Trifolium michelianum savi. - histological observations on adventitious shoot induction

2014 ◽  
Vol 65 (3-4) ◽  
pp. 261-266 ◽  
Author(s):  
Robert Konieczny
Keyword(s):  
Plant Regeneration ◽  
Embryo Culture ◽  
Callus Formation ◽  
Immature Embryo ◽  
Adventitious Shoot ◽  
Meristematic Tissue ◽  
Whole Plant ◽  
Half Strength ◽  
Immature Embryo Culture ◽  
Trifolium Michelianum

Whole plant regeneration via organogenesis in immature embryo culture of <em>Trifolium michelianum</em> Savi. was obtained. The shoots were induced directly from the hypocotyl or indirectly through callus on EC6 medium supplemented with 0.5 and 5 mg/l BAP, respectively. Sporadically the callus formation was preceded by shoot regeneration. Both shoots obtained directly and via callus regenerated into plants when subcultured on half-strength Murashige and Skoog (MS) medium, free of growth regulators. Histological observations revealed that the shoots were induced directly from the ring of meristematic tissue which was formed at the periphery of the hypocotyl or indirectly from the superficial cells of the callus. Irrespective of the mode of regeneration the shoots were of multicellular origin.

scholarly journals Factors influencing somatic embryogenesis and regeneration ability in somatic tissue culture of spring and winter rye

2008 ◽  
Vol 13 (4) ◽  
pp. 363 ◽  
Author(s):  
R. MA ◽  
S. PULLI
Keyword(s):  
Tissue Culture ◽  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Developmental Stage ◽  
Embryogenic Callus ◽  
Immature Embryo ◽  
Green Plant ◽  
Somatic Tissue ◽  
Winter Rye ◽  
Effi Ciency

Rye is an important crop in Northern and Eastern Europe. However, the application of various biotechnologies in rye breeding has been limited duo to its recalcitrant in tissue culture. In order to improve somatic tissue effi ciency, key factors affecting somatic embryogenesis and reproducible green plant regeneration of rye (Secale cereale L.) were evaluated and optimised. In this study, a total 27 rye genotypes including 10 spring and 17 winter genotypes were involved in the investigation. Genotype, culture medium, sugar, gel agent and auxin infl uenced somatic embryogenesis of immature embryo signifi cantly. One-two weeks cold pretreatment of young embryo enhanced somatic embryogenesis and green plant regeneration. In culture of immature embryos, infl orescences and leaf segments of the seedlings, explants signifi cantly infl uenced the culture effi ciency. Highest embryogenic callus yield resulted from rye immature embryo as explant compared to young infl orescence and leaf segment of seedling. Developmental stage of embryo played an important role in somatic embryogenesis. Late spherical coleoptile stage (embryo size 0.5–1mm in length) was optimal developmental stage of immature embryo for culture. Morphogenetic potential of embryogenic callus decreased with an increasing number of subcultures, and this ability could be maintained in vitro for a maximum of 8 months of culturing.;

scholarly journals The evaluation of java fine flavor cocoa propagation through somatic embryogenesis technique for germplasm preservation

2021 ◽  
Vol 306 ◽  
pp. 01056
Author(s):  
Sulistyani Pancaningtyas
Keyword(s):  
Somatic Embryogenesis ◽  
Embryogenic Callus ◽  
Somatic Embryos ◽  
Callus Formation ◽  
Basic Structure ◽  
Embryonic Cells ◽  
Regeneration Rate ◽  
Germplasm Preservation ◽  
Embryogenic Callus Formation ◽  
Secondary Somatic Embryos

Somatic embryogenesis is one of the newest technology that applied for the mass production of cocoa. This research aims to evaluate the regeneration rate of somatic embryos through somatic embryogenesis propagation techniques on java fine flavor cocoa. Cultivars in this study are ICCRI 01, ICCRI 02, DR 1, DR 2, DRC 16, DR 38, PNT 16, and PNT 30. Observations include parameters to determine the percentage of primary callus and embryogenic callus formation and the number of somatic embryos produced. Based on data, the ability of callus to produce primary embryos is highly dependent on plant cultivars and explant sources. Five cultivars showed a higher regeneration rate using explants from the petal part, while the rest showed a higher regeneration rate using explants from the staminode section. Embryogenic callus from each cacao cultivar has the same basic structure: a nodular friable structure consisting of many embryonic cells. Some fine flavor cacao cultivars that were able to produce callus and primary somatic embryos could not produce secondary somatic embryos and plantlets. However, two cultivars, which had low potential in producing primary embryos, had the high ability to produce secondary somatic embryos and develop into plantlets.

scholarly journals INDUCTION AND MORPHOLOGICAL CHARACTERISTICS ASSOCIATED WITH EMBRYOGENESIS OF OENANTHE STOLONIFERA DC.

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 693e-693
Author(s):  
Ji-Weon Lee ◽  
Byoung-Yil Lee
Keyword(s):  
Somatic Embryogenesis ◽  
Embryogenic Callus ◽  
Somatic Embryos ◽  
Callus Formation ◽  
Morphological Characteristics ◽  
Liquid Media ◽  
Friable Embryogenic Callus ◽  
Development And Differentiation ◽  
Rapid Mass ◽  
Embryogenic Callus Formation

The study was carried out to examine the appropriate media, explant sources, and suitable growth regulators for somatic embryogenesis to establish a rapid mass production system via somatic embryogenesis in Oenanthe stolonifera DC. Modified MS media containing higher concentrations of NO3-N were more effective for the formation and development of the somatic embryos from embryogenic callus. Liquid media were more effective for the production of somatic embryos than solidified media. Immature florets were found to be the most competent explant sources for embryogenic callus formation. 2,4-D at 1mg/l was highly effective for the formation of embryogenic callus but inhibitory for the development and differentiation of somatic embryo. Somatic embryos were developed from the translucent and friable embryogenic callus. Addition of BA promoted the callus growth synefgistically with NAA and 2,4-D, but the production of embryogenic callus was inhibited by BA.

scholarly journals Optimization of Protocols for In Vitro Regeneration of Sugarcane (Saccharum officinarum)

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Shakra Jamil ◽  
Rahil Shahzad ◽  
Ghulam Mohyuddin Talha ◽  
Ghazala Sakhawat ◽  
Sajid-ur-Rahman ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Embryogenic Callus ◽  
In Vitro Propagation ◽  
Callus Formation ◽  
Shoot Elongation ◽  
Root Induction ◽  
Rooting Media ◽  
Embryogenic Callus Formation

Sugarcane contributes 60–70% of annual sugar production in the world. Somaclonal variation has potential to enhance genetic variation present within a species. Present study was done to optimize an in vitro propagation protocol for sugarcane. The experiments included four varieties, 9 callus induction media, 27 regeneration media, and 9 root induction media under two-factor factorial CRD. Data were recorded on callus induction, embryogenic callus formation, shoot elongation (cm), root induction, and plant regeneration. Statistically significant differences existed between genotypes and treatments for callus induction (%), embryogenic callus formation (%), shoot elongation (cm), root induction, and plant regeneration (%). All parameters showed dependency on genotypes, culture media, and their interaction. Highest callus induction (95%) embryogenic callus formation (95%) was observed in callus induction media 5. Highest plantlet regeneration (98.9%) capacity was observed in regeneration media 11 whereas maximum shoot elongation (12.13 cm) and root induction (8.32) were observed in rooting media 4. G1 showed best response for all traits and vice versa for G4. Hence it was concluded that G1, callus induction media 5, regeneration media 11, and rooting media 4 are the best conditions for in vitro propagation of sugarcane.

scholarly journals Embryogenic Callus Induction and Regeneration of Elite Bangladeshi Indica Rice Cultivars

1970 ◽  
Vol 17 (1) ◽  
pp. 65-70 ◽  
Author(s):  
ME Hoque ◽  
MS Ali ◽  
NH Karim
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Embryogenic Callus ◽  
Callus Formation ◽  
Indica Rice ◽  
Rice Cultivars ◽  
Embryogenic Calli ◽  
Rice Callus ◽  
Embryogenic Callus Induction ◽  
Embryogenic Callus Formation

Significant variations were observed among six elite Bangladeshi Indica rice cultivars tested in relation to total callus induction frequency (p = 0.017), embryogenic callus formation frequency (p = 0.001) and subsequent plant regeneration responses (p = 0.005). In all the cases, embryogenic callus formation frequency was much more less than the total callus (embryogenic + non-embryonegic) formation frequency. The embryogenic calli derived from mature seed embryos produced green plants, successfully established in soil and produced fertile seeds.Key words: Indica rice, Callus induction, Plant regeneration, Genotypic variationsDOI = 10.3329/ptcb.v17i1.1122Plant Tissue Cult. & Biotech. 17(1): 65-70, 2007 (June)

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