Wheat Immature Embryo Culture for Embryogenic Callus Induction

2002 ◽  
Vol 2 (8) ◽  
pp. 520-521 ◽  
Author(s):  
Kamil Haliloglu .
Keyword(s):  
Embryo Culture ◽  
Callus Induction ◽  
Embryogenic Callus ◽  
Immature Embryo ◽  
Immature Embryo Culture ◽  
Embryogenic Callus Induction

scholarly journals Embryogenic Callus Induction of Aquilaira Malaccensis Lam. and Aquilaria Subintegra Ding Hou

2019 ◽  
Vol 9 (1) ◽  
pp. 5746-5751
Keyword(s):  
Callus Induction ◽  
Embryogenic Callus ◽  
Callus Formation ◽  
Dichlorophenoxyacetic Acid ◽  
Aseptic Culture ◽  
Aquilaria Malaccensis ◽  
The Family ◽  
Commercially Important ◽  
Embryogenic Callus Induction ◽  
2,4 Dichlorophenoxyacetic Acid

Aquilaria malaccensis Lam. and Aquilaria subintegra Ding Hou belong to the family of Thymelaeaceae which is commonly known as gaharu or agarwood. It is a commercially important tree and identified as a potential aromatic plant. The overwhelming responses in the lodging sector reduce gaharu species in the forest. Mass propagation through plant tissue culture technology will substitute this problem. The present study was conducted to investigate the embryogenic callus induction between these two species. The most optimum sterilization method for both species was sodium hypochlorite 5.0% which gave the highest percentage of aseptic culture (95%) with the absence of tissue browning. The leaves of both species were cultured on Murashige and Skoog, (1962) (MS) media supplemented with combination of various concentrations of 6-benzylaminopurine (BAP) (0.5, 1.0, 2.0 and 2.5 mg/L) and 2,4-dichlorophenoxyacetic acid (2, 4-D) (0.5, 1.0, 1.5 and 2.0 mg/L) and kept under dark condition. The explants produced embryogenic, white and compact callus at the end cut of the explants after two weeks of culture in all treatments. The highest frequency of embryogenic callus formation was observed in explants cultured on 2.0 mg/L BAP and 0.5 mg/L 2,4-D for both species. From the present study, the optimum sterilization technique and embryogenic callus induction for A. malaccensis Lam. and A. subintegra were established.

Paucity of somaclonal variation from immature embryo culture of barley

1989 ◽  
Vol 40 (6) ◽  
pp. 1155 ◽  
Author(s):  
DJ Luckett ◽  
D Rose ◽  
E Knights
Keyword(s):  
Tissue Culture ◽  
Somaclonal Variation ◽  
Embryo Culture ◽  
Field Trial ◽  
Immature Embryo ◽  
Immature Embryos ◽  
Golden Promise ◽  
Immature Embryo Culture ◽  
Tissue Origin ◽  
Regenerant Plants

Intact immature embryos of barley (cv. Golden Promise) and component tissues (the scutellum and embryonic axis) were cultured to produce callus. Regenerant plants were obtained from this callus and SC2 families raised. These families were examined in a field trial to search for somaclonal variation. No obvious variants were found confirming our previous unpublished results. The lack of somaclonal variation generated by barley tissue culture (which is in contrast to other species) was not a result of the tissue origin of the regeneration event.

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