Production of Transgenic Grapevine (Vitis vinifera L. cv. Koshusanjaku) Plants by Co-cultivation of Embryogenic Calli with Agrobacterium tumefaciens and Selecting Secondary Embryos

Crown gall was found mostly on vinifera grape (Vitis vinifera L.) cultivars in Ontario vineyards. The galls were usually small and aggregated along the trunk and cane; large woody galls were less common. Vines affected by crown gall died back in 3–4 years after planting. All the nine strains of Agrobacterium tumefaciens (Smith and Townsend) Conn isolated from galls corresponded to biotype 3 in determinative tests and were pathogenic on Datura, tobacco, tomato, and sunflower plants. Eight strains were sensitive to agrocin 84 on amended Stonier's medium. They were suppressed if inoculation was preceded 6 h earlier by Agrobacterium radiobacter strain K84 at wound sites on Datura and tomato plants.

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The Establishment of an Agrobacterium-Mediated Transformation Platform for the Non-Embryogenic Calli of Vitis vinifera L.

Agricultural Sciences in China ◽

10.1016/s1671-2927(11)60051-5 ◽

2011 ◽

Vol 10 (5) ◽

pp. 686-694 ◽

Cited By ~ 2

Author(s):

Feng-xia ZHAO ◽

Shang-wu CHEN ◽

Avihai Perl ◽

Ru DAI ◽

Hai-ying XU ◽

...

Keyword(s):

Vitis Vinifera ◽

Vitis Vinifera L ◽

Embryogenic Calli ◽

Agrobacterium Mediated Transformation

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Vascular-specific expression of GUS and GFP reporter genes in transgenic grapevine (Vitis vinifera L. cv. Albariño) conferred by the EgCCR promoter of Eucalyptus gunnii

Plant Physiology and Biochemistry ◽

10.1016/j.plaphy.2011.02.005 ◽

2011 ◽

Vol 49 (4) ◽

pp. 413-419 ◽

Cited By ~ 19

Author(s):

Jorge Gago ◽

Jacqueline Grima-Pettenati ◽

Pedro Pablo Gallego

Keyword(s):

Vitis Vinifera ◽

Reporter Genes ◽

Vitis Vinifera L ◽

Specific Expression ◽

Gfp Reporter ◽

Transgenic Grapevine

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TRANSFORMATION OF EMBRYOGENIC CALLUS AND TRANSGENIC PLANT REGENERATION IN TABLE GRAPEVINE 'SUGRAONE' (VITIS VINIFERA L.): EFFECT OF AGROBACTERIUM TUMEFACIENS STRAIN

Acta Horticulturae ◽

10.17660/actahortic.2009.827.71 ◽

2009 ◽

pp. 415-420 ◽

Cited By ~ 1

Author(s):

A.J. López-Pérez ◽

J. Carreño ◽

M. Dabauza

Keyword(s):

Agrobacterium Tumefaciens ◽

Plant Regeneration ◽

Transgenic Plant ◽

Vitis Vinifera ◽

Embryogenic Callus ◽

Vitis Vinifera L

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Producing Transgenic `Thompson Seedless' Grape (Vitis vinifera L.) Plants

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.121.4.616 ◽

1996 ◽

Vol 121 (4) ◽

pp. 616-619 ◽

Cited By ~ 48

Author(s):

R. Scorza ◽

J.M. Cordts ◽

D.J. Gray ◽

D. Gonsalves ◽

R.L. Emershad ◽

...

Keyword(s):

Vitis Vinifera ◽

Somatic Embryos ◽

Vitis Vinifera L ◽

Thompson Seedless ◽

Tomato Ringspot Virus ◽

Seedless Grape ◽

Secondary Embryos ◽

Foreign Genes ◽

Transgenic Embryos

Transgenic grape plants were regenerated from somatic embryos derived from leaves of in vitro-grown plants of `Thompson Seedless' grape (Vitis vinifera L.) plants. Somatic embryos were either exposed directly to engineered Agrobacterium tumefaciens or they were bombarded twice with 1-μm gold particles and then exposed to A. tumefaciens. Somatic embryos were transformed with either the lytic peptide Shiva-1 gene or the tomato ringspot virus (TomRSV) coat protein (CP) gene. After cocultivation, secondary embryos proliferated on Emershad/Ramming proliferation (ERP) medium for 6 weeks before selection on ERP medium containing 40 μg·mL-1 kanamycin (kan). Transgenic embryos were identified after 3 to 5 months under selection and allowed to germinate and develop into rooted plants on woody plant medium containing 1 μm 6-benzylaminopurine, 1.5% sucrose, 0.3% activated charcoal, and 0.75% agar. Integration of the foreign genes into these grapevines was verified by growth in the presence of kanamycin (kan), positive β-glucuronidase (GUS) and polymerase chain-reaction (PCR) assays, and Southern analysis.

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