scholarly journals QUANTITATIVE CONFOCAL LASER SCANNING MICROSCOPY

2011 ◽  
Vol 25 (3) ◽  
pp. 111 ◽  
Author(s):  
Merete Krog Raarup ◽  
Jens Randel Nyengaard
Keyword(s):  
Confocal Laser Scanning Microscopy ◽  
Laser Scanning ◽  
3D Structure ◽  
Correlation Spectroscopy ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Scanning Microscopy ◽  
Confocal Laser ◽  
Two Photon ◽  
Photon Excitation

This paper discusses recent advances in confocal laser scanning microscopy (CLSM) for imaging of 3D structure as well as quantitative characterization of biomolecular interactions and diffusion behaviour by means of one- and two-photon excitation. The use of CLSM for improved stereological length estimation in thick (up to 0.5 mm) tissue is proposed. The techniques of FRET (Fluorescence Resonance Energy Transfer), FLIM (Fluorescence Lifetime Imaging Microscopy), FCS (Fluorescence Correlation Spectroscopy) and FRAP (Fluorescence Recovery After Photobleaching) are introduced and their applicability for quantitative imaging of biomolecular (co-)localization and trafficking in live cells described. The advantage of two-photon versus one-photon excitation in relation to these techniques is discussed.

scholarly journals Confocal Laser Scanning Microscopy and Two Photon Excitation Microscopy as Tools to Study Testate Amoebae

2010 ◽  
Vol 16 (S2) ◽  
pp. 1142-1143
Author(s):  
Z Burdíková ◽  
M Čapek ◽  
P Ostasou ◽  
EAD Mitchell ◽  
J Machač ◽  
...  
Keyword(s):  
Confocal Laser Scanning Microscopy ◽  
Laser Scanning ◽  
Testate Amoebae ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Scanning Microscopy ◽  
Confocal Laser ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

Extended abstract of a paper presented at Microscopy and Microanalysis 2010 in Portland, Oregon, USA, August 1 – August 5, 2010.

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