scholarly journals Visualisation of the copepod female reproductive system using confocal laser scanning microscopy and two-photon microscopy

2012 ◽  
Vol 32 (5) ◽  
pp. 685-692 ◽  
Author(s):  
Susan C. Fitzer ◽  
Robert C. Upstill-Goddard ◽  
John D. D. Bishop ◽  
Anthony S. Clare ◽  
Matthew G. Bentley ◽  
...  
2010 ◽  
Vol 91 (2) ◽  
pp. 139-149 ◽  
Author(s):  
Sthefane D’ávila ◽  
Pedro Paulo de Abreu Manso ◽  
Elisabeth Cristina de Almeida Bessa ◽  
Maria de Lurdes de Azevedo Rodrigues ◽  
Roberto Júnio Pedroso Dias

2004 ◽  
Vol 95 (1) ◽  
pp. 43-49 ◽  
Author(s):  
Renata Heisler Neves ◽  
Carla de Lamare Biolchini ◽  
José Roberto Machado-Silva ◽  
Jorge José Carvalho ◽  
Thiago Braga Branquinho ◽  
...  

2010 ◽  
Vol 16 (S2) ◽  
pp. 1142-1143
Author(s):  
Z Burdíková ◽  
M Čapek ◽  
P Ostasou ◽  
EAD Mitchell ◽  
J Machač ◽  
...  

Extended abstract of a paper presented at Microscopy and Microanalysis 2010 in Portland, Oregon, USA, August 1 – August 5, 2010.


2011 ◽  
Vol 25 (3) ◽  
pp. 111 ◽  
Author(s):  
Merete Krog Raarup ◽  
Jens Randel Nyengaard

This paper discusses recent advances in confocal laser scanning microscopy (CLSM) for imaging of 3D structure as well as quantitative characterization of biomolecular interactions and diffusion behaviour by means of one- and two-photon excitation. The use of CLSM for improved stereological length estimation in thick (up to 0.5 mm) tissue is proposed. The techniques of FRET (Fluorescence Resonance Energy Transfer), FLIM (Fluorescence Lifetime Imaging Microscopy), FCS (Fluorescence Correlation Spectroscopy) and FRAP (Fluorescence Recovery After Photobleaching) are introduced and their applicability for quantitative imaging of biomolecular (co-)localization and trafficking in live cells described. The advantage of two-photon versus one-photon excitation in relation to these techniques is discussed.


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