Evaluation of the Two Different Real Time Polymerase Chain Reaction Methods Used for BK Virus (BKV) Quantification and BKV Genotype Assignment

2019 ◽  
Vol 53 (3) ◽  
pp. 285-296
Author(s):  
Aylin ERMAN DALOĞLU ◽  
Derya MUTLU ◽  
İmran SAĞLIK ◽  
Rabia CAN SARINOĞLU ◽  
Esvet MUTLU ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Bk Virus ◽  
Chain Reaction ◽  
Genotype Assignment ◽  
Polymerase Chain

Quantitative Real-Time Polymerase Chain Reaction Detection of BK Virus Using Labeled Primers

2010 ◽  
Vol 134 (3) ◽  
pp. 444-448 ◽  
Author(s):  
Zhengming Gu ◽  
Jianmin Pan ◽  
Matthew J. Bankowski ◽  
Randall T. Hayden
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Real Time Pcr ◽  
Quantitative Polymerase Chain Reaction ◽  
Bk Virus ◽  
Clinical Samples ◽  
Quantitative Detection ◽  
Chain Reaction ◽  
Pcr Method ◽  
Polymerase Chain

Abstract Context.—BK virus infections among immunocompromised patients are associated with disease of the kidney or urinary bladder. High viral loads, determined by quantitative polymerase chain reaction (PCR), have been correlated with clinical disease. Objective.—To develop and evaluate a novel method for real-time PCR detection and quantification of BK virus using labeled primers. Design.—Patient specimens (n = 54) included 17 plasma, 12 whole blood, and 25 urine samples. DNA was extracted using the MagNA Pure LC Total Nucleic Acid Isolation Kit (Roche Applied Science, Indianapolis, Indiana); sample eluate was PCR-amplified using the labeled primer PCR method. Results were compared with those of a user-developed quantitative real-time PCR method (fluorescence resonance energy transfer probe hybridization). Results.—Labeled primer PCR detected less than 10 copies per reaction and showed quantitative linearity from 101 to 107 copies per reaction. Analytical specificity of labeled primer PCR was 100%. With clinical samples, labeled primer PCR demonstrated a trend toward improved sensitivity compared with the reference method. Quantitative assay comparison showed an R2 value of 0.96 between the 2 assays. Conclusions.—Real-time PCR using labeled primers is highly sensitive and specific for the quantitative detection of BK virus from a variety of clinical specimens. These data demonstrate the applicability of labeled primer PCR for quantitative viral detection and offer a simplified method that removes the need for separate oligonucleotide probes.

Investigation of BK Virus by Real-Time Polymerase Chain Reaction in Patients With Allogeneic Bone Marrow Transplantation

2018 ◽  
Vol 50 (5) ◽  
pp. 1510-1513 ◽  
Author(s):  
F. Ekşi ◽  
T. Karslıgil ◽  
İ. Gündeş ◽  
M. Sağlam ◽  
M.P. Kırık ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Polymerase Chain Reaction ◽  
Bone Marrow Transplantation ◽  
Real Time ◽  
Marrow Transplantation ◽  
Allogeneic Bone Marrow Transplantation ◽  
Bk Virus ◽  
Allogeneic Bone ◽  
Chain Reaction ◽  
Polymerase Chain

Detection and quantitation of BK virus DNA by real-time polymerase chain reaction in the LT-ag gene in adult renal transplant recipients

2006 ◽  
Vol 131 (1) ◽  
pp. 21-27 ◽  
Author(s):  
Ali Si-Mohamed ◽  
Jérôme Le Goff ◽  
Nathalie Désiré ◽  
Sarah Maylin ◽  
Denis Glotz ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Renal Transplant ◽  
Real Time ◽  
Bk Virus ◽  
Renal Transplant Recipients ◽  
Transplant Recipients ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Real-Time Polymerase Chain Reaction and Laser Capture Microdissection for the Diagnosis of BK Virus Infection in Renal Allografts

2005 ◽  
Vol 124 (4) ◽  
pp. 537-542 ◽  
Author(s):  
Oyedele A. Adeyi ◽  
Dorothy R. Belloni ◽  
Scott D. Dufresne ◽  
Alan R. Schned ◽  
Gregory J. Tsongalis
Keyword(s):  
Polymerase Chain Reaction ◽  
Virus Infection ◽  
Real Time ◽  
Laser Capture Microdissection ◽  
Bk Virus ◽  
Chain Reaction ◽  
Renal Allografts ◽  
Polymerase Chain ◽  
Laser Capture

1504: Molecular Diagnosis and Staging of Prostate Cancer Using Clusterin in Real Time Reverse Transcription Polymerase Chain Reaction (RT-PCR)

2006 ◽  
Vol 175 (4S) ◽  
pp. 485-486
Author(s):  
Sabarinath B. Nair ◽  
Christodoulos Pipinikas ◽  
Roger Kirby ◽  
Nick Carter ◽  
Christiane Fenske
Keyword(s):  
Prostate Cancer ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Molecular Diagnosis ◽  
Reverse Transcription ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain

Control of the efficiency of instrument-cleaning using real-time Polymerase Chain Reaction

2015 ◽  
Vol 140 (S 01) ◽  
Author(s):  
MG Stoleriu ◽  
M Avci-Adali ◽  
HP Wendel ◽  
C Schlensak ◽  
T Walker
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Chain Reaction ◽  
Polymerase Chain
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