scholarly journals A contribution to an investigation of nitrogen metabolism of some thermophilic and thermophilous soil fungi

2014 ◽  
Vol 14 (1-2) ◽  
pp. 157-161
Author(s):  
Maria Gładoch
Keyword(s):  
Aspergillus Fumigatus ◽  
Nitrogen Metabolism ◽  
Soil Fungi ◽  
Humicola Lanuginosa ◽  
Sporotrichum Thermophile ◽  
Malbranchea Pulchella ◽  
Different Sources

The author has examined the assimilation of nitrogen on the basis of different sources of nitrogen and the secretion of nitrite to the substrate by some soil fungi. The following species of thermophilic and thermophilous fungi have been investigated: <i>Malbranchea pulchella</i> Saccardo et Penzig var. <i>sulfurea</i> Miehe, <i>Humicola lanuginosa</i> (Griffon et Maublanc) Bunce. <i>Sporotrichum thermophile</i> Apinis, <i>Thielavia sepedonium</i> Emmons, <i>Paecilomyces varioti</i> Bainier, <i>Aspergillus fumigatus</i> Fresenius, A. terreus Thom.

scholarly journals Population structure and diversity of the pathogenic fungus Aspergillus fumigatus isolated from different sources and geographic origins

2009 ◽  
Vol 104 (3) ◽  
pp. 427-433 ◽  
Author(s):  
Esperanza Duarte-Escalante ◽  
Gerardo Zúñiga ◽  
Oscar Nava Ramírez ◽  
Susana Córdoba ◽  
Nicolás Refojo ◽  
...  
Keyword(s):  
Population Structure ◽  
Aspergillus Fumigatus ◽  
Pathogenic Fungus ◽  
Geographic Origins ◽  
Different Sources

scholarly journals Identification of Hypoxia-Inducible Target Genes of Aspergillus fumigatus by Transcriptome Analysis Reveals Cellular Respiration as an Important Contributor to Hypoxic Survival

2014 ◽  
Vol 13 (9) ◽  
pp. 1241-1253 ◽  
Author(s):  
Kristin Kroll ◽  
Vera Pähtz ◽  
Falk Hillmann ◽  
Yakir Vaknin ◽  
Wolfgang Schmidt-Heck ◽  
...  
Keyword(s):  
Aspergillus Fumigatus ◽  
Nitrogen Metabolism ◽  
Transcriptome Analysis ◽  
Metal Ion ◽  
Target Genes ◽  
Cellular Respiration ◽  
Cell Wall Polysaccharide ◽  
Content Type ◽  
Nitrite Reductase Gene ◽  
Reductase Gene

ABSTRACT Aspergillus fumigatus is an opportunistic, airborne pathogen that causes invasive aspergillosis in immunocompromised patients. During the infection process, A. fumigatus is challenged by hypoxic microenvironments occurring in inflammatory, necrotic tissue. To gain further insights into the adaptation mechanism, A. fumigatus was cultivated in an oxygen-controlled chemostat under hypoxic and normoxic conditions. Transcriptome analysis revealed a significant increase in transcripts associated with cell wall polysaccharide metabolism, amino acid and metal ion transport, nitrogen metabolism, and glycolysis. A concomitant reduction in transcript levels was observed with cellular trafficking and G-protein-coupled signaling. To learn more about the functional roles of hypoxia-induced transcripts, we deleted A. fumigatus genes putatively involved in reactive nitrogen species detoxification ( fhpA ), NAD + regeneration ( frdA and osmA ), nitrogen metabolism ( niaD and niiA ), and respiration ( rcfB ). We show that the nitric oxygen (NO)-detoxifying flavohemoprotein gene fhpA is strongly induced by hypoxia independent of the nitrogen source but is dispensable for hypoxic survival. By deleting the nitrate reductase gene niaD , the nitrite reductase gene niiA , and the two fumarate reductase genes frdA and osmA , we found that alternative electron acceptors, such as nitrate and fumarate, do not have a significant impact on growth of A. fumigatus during hypoxia, but functional mitochondrial respiratory chain complexes are essential under these conditions. Inhibition studies indicated that primarily complexes III and IV play a crucial role in the hypoxic growth of A. fumigatus .

Production of amylase by soil fungi and partial biochemical characterization of amylase of a selected strain (Aspergillus fumigatus Fresenius)

1993 ◽  
Vol 39 (7) ◽  
pp. 681-685 ◽  
Author(s):  
Claudia M. Domingues ◽  
Rosane M. Peralta
Keyword(s):  
Aspergillus Fumigatus ◽  
Soil Fungi ◽  
Biochemical Characterization ◽  
Amylase Activity ◽  
Carbon Sources ◽  
Lignocellulosic Materials ◽  
Liquid Media ◽  
Maximal Yield ◽  
High Enzymatic Activity

Filamentous fungi from soil were screened for their ability to produce amylases in semisolid and liquid media with wheat bran. A selected strain identified as Aspergillus fumigatus Fresenius showed high enzymatic activity for α-amylase and glucoamylase. The maximal yield of these amylases was obtained when lignocellulosic materials were the carbon sources. The optimal pH and temperature were 6.0 and 50 °C, respectively, for both enzymes. α-Amylase activity was more thermostable than glucoamylase activity.Key words: amylolitic fungi, α-amylase, glucoamylase, Aspergillus fumigatus.

Use of a microbial toxicity test (Microtox®) to determine the toxigenicity of Aspergillus fumigatus strains isolated from different sources

Toxicon ◽  
2009 ◽  
Vol 53 (7-8) ◽  
pp. 729-733 ◽  
Author(s):  
Patricia Alba ◽  
Sebastián Sánchez-Fortún ◽  
Sergio Alvarez-Perez ◽  
Jose L. Blanco ◽  
Marta E. García
Keyword(s):  
Aspergillus Fumigatus ◽  
Toxicity Test ◽  
Microbial Toxicity ◽  
Different Sources

scholarly journals mSphere of Influence: the Importance of Metabolism for Pathogen Adaptation to Host-Imposed Stresses

mSphere ◽  
2019 ◽  
Vol 4 (4) ◽  
Author(s):  
Jorge Amich
Keyword(s):  
Gene Expression ◽  
Invasive Aspergillosis ◽  
Aspergillus Fumigatus ◽  
Nitrogen Metabolism ◽  
Content Type

ABSTRACT Jorge Amich studies several aspects of sulfur and nitrogen metabolism in Aspergillus fumigatus, with the ultimate aim of identifying targets for the development of novel antifungals. In this mSphere of Influence article, he reflects on how “Sub-Telomere Directed Gene Expression during Initiation of Invasive Aspergillosis” (A. McDonagh, N. D. Fedorova, J. Crabtree, Y. Yu, S. Kim, et al., PLoS Pathog 4:e1000154, 2008, https://doi.org/10.1371/journal.ppat.1000154) impacted his thinking about in vivo metabolism and how to investigate it.

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