scholarly journals Ectomycorrhizae of Tomentella badia: description and molecular identification

2013 ◽  
Vol 48 (2) ◽  
pp. 155-171 ◽  
Author(s):  
Andrea Binder ◽  
Derek Peršoh ◽  
Nourou S. Yorou ◽  
Rita Verma ◽  
Claus Bässler ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Picea Abies ◽  
Molecular Identification ◽  
Its Region ◽  
Molecular Phylogenetic Analysis ◽  
Functional Characteristics ◽  
Molecular Phylogenetic ◽  
Dark Blue

Species within the genera <em>Tomentella </em>are among the most important ECM in forests. However, our knowledge about their functional characteristics is still rather limited. The ectomycorrhizae of <em>Tomentella badia </em>on <em>Picea abies </em>are described here in detail and compared to the non-identified ECM <em>Piceirhiza obscura</em>. A pseudoparenchymatous mantle formed by epidermoid cells is covered by heaps of epidermoid cells. This mantle type is regarded as a new one and designated as mantle type R. Many cells filled with dark blue contents and/or blue granules, together with clampless hyphae, are distinct characters of these ectomycorrhizae. Molecular-phylogenetic analysis of the ITS region was used for identification.

A new species of Conocybe (Bolbitaceae) from Punjab, Pakistan

Phytotaxa ◽  
2019 ◽  
Vol 402 (5) ◽  
pp. 251
Author(s):  
AIMAN IZHAR ◽  
HIRA BASHIR ◽  
ABDUL NASIR KHALID
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Its Region ◽  
Molecular Phylogenetic Analysis ◽  
Molecular Evidence ◽  
Fruiting Bodies ◽  
Germ Pore ◽  
Central Disc ◽  
Molecular Phylogenetic ◽  
A New Species

Conocybe punjabensis sp. nov., a species belonging to the saprobic family Bolbitaceae, is illustrated and described from Pakistan based on morphology and molecular evidence. The species can be distinguished on the basis of medium-sized fruiting bodies, a pileus with a dark brown central disc, a fibrillose stipe, forked lamellae near pileus margin, ellipsoid angular basidiospores with an apical germ pore, cheilocystidia and pleurocystidia varying from catenulate, lecythiform to clavate, and lecythiform caulocystidia. Molecular phylogenetic analysis of the nuclear ribosomal ITS region also supports the recognition of this new species in Conocybe. Detailed descriptions, photographs, illustrations and comparison with allied taxa are given.

Infundibulicybe macrospora sp. nov., a new and noteworthy species from Himalayan moist temperate forests of Pakistan

Phytotaxa ◽  
2020 ◽  
Vol 452 (4) ◽  
pp. 268-277
Author(s):  
MUHAMMAD ALI ◽  
JUNAID KHAN ◽  
HIRA BASHIR ◽  
ABDUL REHMAN NIAZI ◽  
HASSAN SHER ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Internal Transcribed Spacer ◽  
Temperate Forests ◽  
Its Region ◽  
Molecular Phylogenetic Analysis ◽  
Molecular Phylogenetic ◽  
A New Species ◽  
Reddish Orange ◽  
Light Orange

Infundibulicybe macrospora, a new species, is described and illustrated from the Himalayan moist temperate forests of Pakistan. The new species can be differentiated from close allies by its reddish orange to light orange pileus, whitish lamellae and stipe, and larger and broader basidiospores. The new species is also supported by molecular phylogenetic analysis inferred from internal transcribed spacer (ITS) region.

scholarly journals The ITS region provides a reliable DNA barcode for identifying reishi/lingzhi (Ganoderma) from herbal supplements

2020 ◽  
Author(s):  
Tess Gunnels ◽  
Matthew Creswell ◽  
Janis McFerrin ◽  
Justen B. Whittall
Keyword(s):  
Phylogenetic Analysis ◽  
Phylogenetic Analyses ◽  
Dna Barcode ◽  
Its Region ◽  
Molecular Phylogenetic Analysis ◽  
Herbal Supplement ◽  
Herbal Supplements ◽  
Expected Size Range ◽  
Molecular Phylogenetic ◽  
Species Authentication

AbstractThe dietary supplement industry is a growing enterprise, valued at over $100 billion by 2025 yet, a recent study revealed that up to 60% of herbal supplements may have substituted ingredients not listed on their labels, some with harmful contaminants. Substituted ingredients make rigorous quality control testing a necessary aspect in the production of supplements. Traditionally, species have been verified morphologically or biochemically, but this is not possible for all species if the identifying characteristics are lost in the processing of the material. One approach to validating plant and fungal ingredients in herbal supplements is through DNA barcoding complemented with a molecular phylogenetic analysis. This method provides an efficient, objective, rigorous and repeatable method for species identification. We employed a molecular phylogenetic analysis for species authentication of the commonly used fungal supplement, reishi (Ganoderma lingzhi), by amplifying and sequencing the nuclear ribosomal internal transcribed spacer regions (ITS) with genus-specific primers. PCR of six powdered samples and one dried sample sold as G. lucidum representing independent suppliers produced single, strong amplification products in the expected size-range for Ganoderma. Both best-hit BLAST and molecular phylogenetic analyses using a reference panel assembled from Genbank clearly identified the predominant fungal DNA was G. lingzhi in all seven herbal supplements. We detected variation in ITS among our samples, but all samples still fall within a large clade of G. lingzhi. ITS is a successful and cost-effective method for DNA-based species authentication that could be used in the herbal supplement industry for this and other fungal and plant species that are otherwise difficult to identify.

scholarly journals Molecular Phylogenetic Analysis of the AIG Family in Vertebrates

Genes ◽  
2021 ◽  
Vol 12 (8) ◽  
pp. 1190
Author(s):  
Yuqi Huang ◽  
Minghao Sun ◽  
Lenan Zhuang ◽  
Jin He
Keyword(s):  
Phylogenetic Analysis ◽  
Gene Family ◽  
Functional Characterization ◽  
Vertebrate Evolution ◽  
Molecular Phylogenetic Analysis ◽  
Whole Genome Duplications ◽  
Genome Duplications ◽  
Molecular Phylogenetic ◽  
Duplication Events ◽  
Inducible Genes

Androgen-inducible genes (AIGs), which can be regulated by androgen level, constitute a group of genes characterized by the presence of the AIG/FAR-17a domain in its protein sequence. Previous studies on AIGs demonstrated that one member of the gene family, AIG1, is involved in many biological processes in cancer cell lines and that ADTRP is associated with cardiovascular diseases. It has been shown that the numbers of AIG paralogs in humans, mice, and zebrafish are 2, 2, and 3, respectively, indicating possible gene duplication events during vertebrate evolution. Therefore, classifying subgroups of AIGs and identifying the homologs of each AIG member are important to characterize this novel gene family further. In this study, vertebrate AIGs were phylogenetically grouped into three major clades, ADTRP, AIG1, and AIG-L, with AIG-L also evident in an outgroup consisting of invertebrsate species. In this case, AIG-L, as the ancestral AIG, gave rise to ADTRP and AIG1 after two rounds of whole-genome duplications during vertebrate evolution. Then, the AIG family, which was exposed to purifying forces during evolution, lost or gained some of its members in some species. For example, in eutherians, Neognathae, and Percomorphaceae, AIG-L was lost; in contrast, Salmonidae and Cyprinidae acquired additional AIG copies. In conclusion, this study provides a comprehensive molecular phylogenetic analysis of vertebrate AIGs, which can be employed for future functional characterization of AIGs.

Molecular phylogenetic analysis of dinoflagellate Scrippsiella trochoidea isolated from the East Asian waters

2010 ◽  
Vol 28 (2) ◽  
pp. 323-328 ◽  
Author(s):  
Xianghai Tang ◽  
Rencheng Yu ◽  
Qingchun Zhang ◽  
Yunfeng Wang ◽  
Tian Yan ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
East Asian ◽  
Molecular Phylogenetic Analysis ◽  
Scrippsiella Trochoidea ◽  
Molecular Phylogenetic
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