scholarly journals Detection of Bacterial Pathogens in River Water Using Multiplex-PCR

10.5772/37004 ◽  
2012 ◽  
Author(s):  
C. N. Wose Kinge ◽  
M. Mbewe ◽  
N. P.
Keyword(s):  
Multiplex Pcr ◽  
River Water ◽  
Bacterial Pathogens

scholarly journals Development of a multiplex PCR for simultaneous detection of Pasteurella multocida, Mannheimia haemolytica and Trueperella pyogenes

2017 ◽  
Vol 65 (3) ◽  
pp. 327-339 ◽  
Author(s):  
Wenlong Zhang ◽  
Xiaodan Liu ◽  
Mengcheng Liu ◽  
Bo Ma ◽  
Li Xu ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Genomic Dna ◽  
Pasteurella Multocida ◽  
Bacterial Pathogens ◽  
Simultaneous Detection ◽  
Laboratory Diagnosis ◽  
Mannheimia Haemolytica ◽  
Bacterial Strains ◽  
Trueperella Pyogenes ◽  
Enrichment Technology

Pasteurella multocida, Mannheimia haemolytica and Trueperella pyogenes are three bacterial pathogens closely associated with the bovine respiratory disease complex (BRDC). In the current study, a multiplex PCR for the simultaneous detection of these three bacteria in cultures was established. After serial optimisation, the detection limit of the method for the genomic DNA of the three bacteria was 40 pg/μl. The method could detect the genomic DNA of these three bacteria but not the genomic DNA of seven other bacterial strains. Together with the bacterial enrichment technology, the multiplex PCR could be used for detecting the three bacteria in animal tissues. This method might be valuable for speeding up laboratory diagnosis and directing the treatment of BRDC to these three bacterial pathogens.

Multiplex PCR amplification and immobilized capture probes for detection of bacterial pathogens and indicators in water

1990 ◽  
Vol 4 (5) ◽  
pp. 353-365 ◽  
Author(s):  
Asim K. Bej ◽  
Meena H. Mahbubani ◽  
Richard Miller ◽  
Joseph L. DiCesare ◽  
Lawrence Haff ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Bacterial Pathogens ◽  
Pcr Amplification

Multiplex PCR assay for the simultaneous detection of bacterial pathogens in rainbow trout

2017 ◽  
Vol 25 (4) ◽  
pp. 1569-1575 ◽  
Author(s):  
Nadia Rajabzadeh ◽  
Mohsen Naeemipour ◽  
Mohsen Seyedabadi
Keyword(s):  
Rainbow Trout ◽  
Multiplex Pcr ◽  
Bacterial Pathogens ◽  
Simultaneous Detection ◽  
Pcr Assay ◽  
Multiplex Pcr Assay

scholarly journals Detection of major diarrheagenic bacterial pathogens by multiplex PCR panels

2015 ◽  
Vol 172 ◽  
pp. 34-40 ◽  
Author(s):  
Åsa Sjöling ◽  
Leila Sadeghipoorjahromi ◽  
Daniel Novak ◽  
Joshua Tobias
Keyword(s):  
Multiplex Pcr ◽  
Bacterial Pathogens
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