Clinical Evaluation of the Multiplex PCR Assay for the Detection of Bacterial Pathogens in Respiratory Specimens from Patients with Pneumonia

2010 ◽  
Vol 13 (1) ◽  
pp. 40 ◽  
Author(s):  
Chae Lim Jung ◽  
Mi Ae Lee ◽  
Wha Soon Chung
Keyword(s):  
Multiplex Pcr ◽  
Clinical Evaluation ◽  
Bacterial Pathogens ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Respiratory Specimens

Multiplex PCR assay for the simultaneous detection of bacterial pathogens in rainbow trout

2017 ◽  
Vol 25 (4) ◽  
pp. 1569-1575 ◽  
Author(s):  
Nadia Rajabzadeh ◽  
Mohsen Naeemipour ◽  
Mohsen Seyedabadi
Keyword(s):  
Rainbow Trout ◽  
Multiplex Pcr ◽  
Bacterial Pathogens ◽  
Simultaneous Detection ◽  
Pcr Assay ◽  
Multiplex Pcr Assay

scholarly journals Development and application of a multiplex PCR assay for rapid detection of 4 major bacterial pathogens in ducks

2013 ◽  
Vol 92 (5) ◽  
pp. 1164-1170 ◽  
Author(s):  
B. Wei ◽  
S.-Y. Cha ◽  
M. Kang ◽  
I.-J. Park ◽  
O.-K. Moon ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Rapid Detection ◽  
Bacterial Pathogens ◽  
Pcr Assay ◽  
Multiplex Pcr Assay

Development and Clinical Evaluation of a New Multiplex PCR Assay for a Simultaneous Diagnosis of Tuberculous and Nontuberculous Mycobacteria

2021 ◽  
Author(s):  
Yeya dit Sadio Sarro ◽  
Matthew A. Butzler ◽  
Fanta Sanogo ◽  
Ousmane Kodio ◽  
Mohamed Tolofoudie ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Clinical Evaluation ◽  
Nontuberculous Mycobacteria ◽  
Pcr Assay ◽  
Multiplex Pcr Assay

scholarly journals Multiplex PCR assay for simultaneous detection of six major bacterial pathogens of rice

2016 ◽  
Vol 120 (5) ◽  
pp. 1357-1367 ◽  
Author(s):  
Z. Cui ◽  
M.R. Ojaghian ◽  
Z. Tao ◽  
K.U. Kakar ◽  
J. Zeng ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Bacterial Pathogens ◽  
Simultaneous Detection ◽  
Pcr Assay ◽  
Multiplex Pcr Assay

scholarly journals Development and clinical evaluation of a new multiplex PCR assay for a simultaneous diagnosis of tuberculous and nontuberculous mycobacteria

EBioMedicine ◽  
2021 ◽  
Vol 70 ◽  
pp. 103527
Author(s):  
Yeya dit Sadio Sarro ◽  
Matthew A Butzler ◽  
Fanta Sanogo ◽  
Ousmane Kodio ◽  
Mohamed Tolofoudie ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Clinical Evaluation ◽  
Nontuberculous Mycobacteria ◽  
Pcr Assay ◽  
Multiplex Pcr Assay

Usefulness of a Multiplex PCR for Detection of Diarrheagenic Escherichia coli in a Diagnostic Microbiology Laboratory Setting

2016 ◽  
Vol 1 (2) ◽  
pp. 38-42 ◽  
Author(s):  
Khairun Nessa ◽  
Dilruba Ahmed ◽  
Johirul Islam ◽  
FM Lutful Kabir ◽  
M Anowar Hossain
Keyword(s):  
Escherichia Coli ◽  
Multiplex Pcr ◽  
Clinical Laboratory ◽  
Proteinase K ◽  
Microbiology Laboratory ◽  
Pcr Assay ◽  
E Coli ◽  
Diarrheagenic Escherichia Coli ◽  
Multiplex Pcr Assay ◽  
Diagnostic Microbiology

A multiplex PCR assay was evaluated for diagnosis of diarrheagenic Escherichia coli in stool samples of patients with diarrhoea submitted to a diagnostic microbiology laboratory. Two procedures of DNA template preparationproteinase K buffer method and the boiling method were evaluated to examine isolates of E. coli from 150 selected diarrhoeal cases. By proteinase K buffer method, 119 strains (79.3%) of E. coli were characterized to various categories by their genes that included 55.5% enteroaggregative E. coli (EAEC), 18.5% enterotoxigenic E. coli (ETEC), 1.7% enteropathogenic E. coli (EPEC), and 0.8% Shiga toxin-producing E. coli (STEC). Although boiling method was less time consuming (<24 hrs) and less costly (<8.0 US $/ per test) but was less efficient in typing E. coli compared to proteinase K method (41.3% vs. 79.3% ; p<0.001). The sensitivity and specificity of boiling method compared to proteinase K method was 48.7% and 87.1% while the positive and negative predictive value was 93.5% and 30.7%, respectively. The majority of pathogenic E. coli were detected in children (78.0%) under five years age with 53.3% under one year, and 68.7% of the children were male. Children under 5 years age were frequently infected with EAEC (71.6%) compared to ETEC (24.3%), EPEC (2.7%) and STEC (1.4%). The multiplex PCR assay could be effectively used as a rapid diagnostic tool for characterization of diarrheagenic E. coli using a single reaction tube in the clinical laboratory setting.Bangladesh J Med Microbiol 2007; 01 (02): 38-42

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