scholarly journals Evaluation of a Multiplex PCR Assay with Molecular Beacon Probes to Rapidly Detect Bacterial Pathogens Directly in Bronchial Alveolar Lavage (BAL) Samples from Patients with Hospital-Acquired Pneumonia (HAP)

2017 ◽  
Vol 4 (suppl_1) ◽  
pp. S614-S614
Author(s):  
Kalyan D Chavda ◽  
Michael Satlin ◽  
Liang Chen ◽  
Bhakti Chavda ◽  
Claudia Manca ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Molecular Beacon ◽  
Bacterial Pathogens ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Hospital Acquired Pneumonia ◽  
Hospital Acquired ◽  
Bronchial Alveolar Lavage

scholarly journals Characteristics of hospital- and community-acquired meticillin-resistant Staphylococcus aureus in Tehran, Iran

2014 ◽  
Vol 63 (6) ◽  
pp. 796-804 ◽  
Author(s):  
Fateh Rahimi ◽  
Mohammad Katouli ◽  
Mohammad Reza Pourshafie
Keyword(s):  
Staphylococcus Aureus ◽  
Multiplex Pcr ◽  
Outpatient Clinic ◽  
Pcr Assay ◽  
Type Iii ◽  
Multiplex Pcr Assay ◽  
Type 3 ◽  
Hospital Acquired ◽  
Staphylococcal Cassette Chromosome

Staphylococcus aureus is a leading cause of hospital-acquired (HA) and community-acquired (CA) infections worldwide. Recently, S. aureus strains resistant to meticillin (MRSA) have become established within both communities. We isolated 314 isolates of MRSA from hospitalized patients in a referral hospital (HA isolates) and 268 isolates from its outpatient clinic (CA isolates) in Tehran, Iran, between February 2008 and December 2010. These isolates were tested for their susceptibility to 17 antibiotics and typed using the PhPlate system. The diversity in the structures of staphylococcal cassette chromosome mec (SCCmec) elements and ccr types was also detected using a multiplex-PCR assay and isolates were examined for the presence of different classes of prophages. Whilst all isolates were resistant to penicillin, the HA isolates were significantly more resistant to all other antibiotics tested than the CA isolates. Isolates carrying only SCCmec type III and ccr type 3 were dominant (91 %), but 20 % of the CA isolates belonging to less prevalent types carried only SCCmec types IVa, c and ccr type 2. These isolates also carried pvl genes and contained SGA prophage type. Our results indicate that whilst the dominant clonal groups of HA- and CA-MRSA belong to SCCmec type III and carry ccr type 3 genes, several distinct but less prevalent types of CA-MRSA carrying SCCmec type IVa, c and type 2 ccr are also found in Tehran. These strains carry pvl genes and the SGA prophage type, a characteristic that might be used as a marker for detection of CA–MRSA in this country.

Multiplex PCR assay for the simultaneous detection of bacterial pathogens in rainbow trout

2017 ◽  
Vol 25 (4) ◽  
pp. 1569-1575 ◽  
Author(s):  
Nadia Rajabzadeh ◽  
Mohsen Naeemipour ◽  
Mohsen Seyedabadi
Keyword(s):  
Rainbow Trout ◽  
Multiplex Pcr ◽  
Bacterial Pathogens ◽  
Simultaneous Detection ◽  
Pcr Assay ◽  
Multiplex Pcr Assay

scholarly journals Development and application of a multiplex PCR assay for rapid detection of 4 major bacterial pathogens in ducks

2013 ◽  
Vol 92 (5) ◽  
pp. 1164-1170 ◽  
Author(s):  
B. Wei ◽  
S.-Y. Cha ◽  
M. Kang ◽  
I.-J. Park ◽  
O.-K. Moon ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Rapid Detection ◽  
Bacterial Pathogens ◽  
Pcr Assay ◽  
Multiplex Pcr Assay

scholarly journals Multiplex PCR assay for simultaneous detection of six major bacterial pathogens of rice

2016 ◽  
Vol 120 (5) ◽  
pp. 1357-1367 ◽  
Author(s):  
Z. Cui ◽  
M.R. Ojaghian ◽  
Z. Tao ◽  
K.U. Kakar ◽  
J. Zeng ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Bacterial Pathogens ◽  
Simultaneous Detection ◽  
Pcr Assay ◽  
Multiplex Pcr Assay

Usefulness of a Multiplex PCR for Detection of Diarrheagenic Escherichia coli in a Diagnostic Microbiology Laboratory Setting

2016 ◽  
Vol 1 (2) ◽  
pp. 38-42 ◽  
Author(s):  
Khairun Nessa ◽  
Dilruba Ahmed ◽  
Johirul Islam ◽  
FM Lutful Kabir ◽  
M Anowar Hossain
Keyword(s):  
Escherichia Coli ◽  
Multiplex Pcr ◽  
Clinical Laboratory ◽  
Proteinase K ◽  
Microbiology Laboratory ◽  
Pcr Assay ◽  
E Coli ◽  
Diarrheagenic Escherichia Coli ◽  
Multiplex Pcr Assay ◽  
Diagnostic Microbiology

A multiplex PCR assay was evaluated for diagnosis of diarrheagenic Escherichia coli in stool samples of patients with diarrhoea submitted to a diagnostic microbiology laboratory. Two procedures of DNA template preparationproteinase K buffer method and the boiling method were evaluated to examine isolates of E. coli from 150 selected diarrhoeal cases. By proteinase K buffer method, 119 strains (79.3%) of E. coli were characterized to various categories by their genes that included 55.5% enteroaggregative E. coli (EAEC), 18.5% enterotoxigenic E. coli (ETEC), 1.7% enteropathogenic E. coli (EPEC), and 0.8% Shiga toxin-producing E. coli (STEC). Although boiling method was less time consuming (<24 hrs) and less costly (<8.0 US $/ per test) but was less efficient in typing E. coli compared to proteinase K method (41.3% vs. 79.3% ; p<0.001). The sensitivity and specificity of boiling method compared to proteinase K method was 48.7% and 87.1% while the positive and negative predictive value was 93.5% and 30.7%, respectively. The majority of pathogenic E. coli were detected in children (78.0%) under five years age with 53.3% under one year, and 68.7% of the children were male. Children under 5 years age were frequently infected with EAEC (71.6%) compared to ETEC (24.3%), EPEC (2.7%) and STEC (1.4%). The multiplex PCR assay could be effectively used as a rapid diagnostic tool for characterization of diarrheagenic E. coli using a single reaction tube in the clinical laboratory setting.Bangladesh J Med Microbiol 2007; 01 (02): 38-42

New approach for the identification of potentially toxigenic Corynebacterium sp. using a multiplex PCR assay

2021 ◽  
pp. 106198
Author(s):  
Sunarno ◽  
Khariri ◽  
Fauzul Muna ◽  
Kambang Sariadji ◽  
Yuni Rukminiati ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Pcr Assay ◽  
New Approach ◽  
Multiplex Pcr Assay ◽  
Corynebacterium Sp
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