scholarly journals Efficient one-step tissue culture protocol for propagation of endemic plant, Lilium martagon var. cattaniae Vis.

2012 ◽  
Vol 11 (8) ◽  
pp. 1862-1867 ◽  
Author(s):  
Skorić Marijana ◽  
Živković Suzana ◽  
Savić Jelena ◽  
Scaron iler Branislav ◽  
Sabovljević Aneta ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Endemic Plant ◽  
One Step ◽  
Culture Protocol

Establishment of Tissue Culture Protocol in Brassica (B. napus L.)

2004 ◽  
Vol 7 (2) ◽  
pp. 277-278 ◽  
Author(s):  
Ihsan Ullah ◽  
Hamid Rashid . ◽  
M. Ramzan Khan .
Keyword(s):  
Tissue Culture ◽  
Culture Protocol

A micro tissue culture test for the titration and neutralization of rubella virus

1969 ◽  
Vol 15 (1) ◽  
pp. 67-71 ◽  
Author(s):  
John Furesz ◽  
Pierre Moreau ◽  
Walter Yarosh
Keyword(s):  
Tissue Culture ◽  
Rubella Virus ◽  
Fetal Calf Serum ◽  
Constant Pressure ◽  
Calf Serum ◽  
Rabbit Kidney ◽  
Tissue Cultures ◽  
Virus Titration ◽  
One Step ◽  
Microneutralization Test

A simple and reproducible micro tissue culture assay has been devised in RK13 and LLC-RK1 rabbit kidney cells for the titration and neutralization of rubella virus. In this "one-step" assay all virus and serum dilutions were prepared with spiral loops in disposable microplates and tissue cultures suspended in medium 199 and 3% horse or fetal calf serum were added to the microcups simultaneously. Micro tissue cultures were kept in a humidified incubator (36 °C) under a constant pressure of 5% CO2 for 8 days and were read microscopically for viral cytopathic changes on the seventh and eighth day. The microneutralization test performed in LLC-RK1 cell cultures was shown to be a reliable method for the detection of small amounts of rubella antibodies in human sera.The micro assay may be also applied to the virus titration of live, attenuated rubella vaccines.

A Reliable Homemade Tissue Culture Protocol for Dendrobium Orchid Cultivation

2015 ◽  
Vol 804 ◽  
pp. 227-230 ◽  
Author(s):  
Duongruitai Nicomrat ◽  
Jackrit Anantasaran
Keyword(s):  
Tissue Culture ◽  
Culture Media ◽  
Nutrient Ratios ◽  
Simple Type ◽  
Tissue Cultures ◽  
Nutritional Values ◽  
Tissue Culture Media ◽  
The Media ◽  
Suitable Technique ◽  
Culture Protocol

Murashige and Skoog (MS) medium, a common tissue culture media for orchids which is mostly supplemented with various natural organic substances with high nutritional values can accelerate the growth of plant tissues. Nevertheless, the knowledge of actual compositions of these natural added substances is limited, causing various culture results. In this study, we have investigated the effects of added natural organic nutrient ratios on 2 orchid tissue cultures, Dendrobium, Dendrobium farmeri Paxt. and Dendrobium griffithianum Lindl. Additionally, simple, affordable medium recipes, MS media supplemented with banana were thus economically sterilized by either a simple type steaming vapor boiler or an autoclave for reliability improvement. The results showed that the orchids grew better in medium supplemented with Namwa banana especially at 150 g/L for Dendrobiumfarmeri Paxt. and 75 g/L for Dendrobium griffithianum Lindl. The suitable steaming procedure at 100°C for 60 minutes was adequate for eliminating most pathogens from the media and helped both orchid seedlings cultivated very well. Moreover, the optimized homemade tissue culture protocol provides fast effectiveness, simplicity, consistently high nutritional values, and practically being suitable technique for ready transferring to the community.

scholarly journals Optimisation of the tissue culture protocol for the endangered Aloe polyphylla

2002 ◽  
Vol 68 (4) ◽  
pp. 424-429 ◽  
Author(s):  
J.C. Chukwujekwu ◽  
C.W. Fennell ◽  
J. van Staden
Keyword(s):  
Tissue Culture ◽  
Aloe Polyphylla ◽  
Culture Protocol

scholarly journals Rapid Evaluation of Pathogenicity in Pseudomonas syringae pv. syringae with a Lilac Tissue Culture Bioassay and Syringomycin DNA Probes

Plant Disease ◽  
1997 ◽  
Vol 81 (8) ◽  
pp. 905-910 ◽  
Author(s):  
Heather J. Scheck ◽  
Marilyn L. Canfield ◽  
Jay W. Pscheidt ◽  
Larry W. Moore
Keyword(s):  
Tissue Culture ◽  
Pacific Northwest ◽  
Pseudomonas Syringae ◽  
Woody Plants ◽  
Dna Probes ◽  
Rapid Evaluation ◽  
Colony Hybridization ◽  
The Pacific ◽  
One Step ◽  
Tissue Culture Plantlets

Losses from diseases caused by Pseudomonas syringae pv. syringae occur on a large number of deciduous woody plants in commercial nurseries in the Pacific Northwest. Bioassays for pathogenicity are one step in the identification of P. syringae pv. syringae and are usually performed on the host of isolation; however, woody plants can take months to develop symptoms. A bioassay with highly susceptible lilac (Syringa vulgaris ‘Sensation’) tissue culture plantlets evaluated pathogenicity in strains of P. syringae pv. syringae isolated from 25 species of deciduous woody plants. DNA colony hybridization with the syrB probe for a syringomycin synthetase gene and the syrD probe for a syringomycin export gene was also evaluated as a method for identifying pathogens. Of 552 strains provisionally identified as P. syringae pv. syringae, 59% were pathogenic in the bioassay and hybridized with the syr probes, while 19% were non-pathogenic and did not hybridize with the syr probes, giving 78% agreement between the two methods. Nine percent of strains were pathogenic in the bioassay but did not hybridize with the syr probes, and 13% were not pathogenic in the bioassay but did hybridize with the syr probes. These methods detected pathogenic strains of P. syringae pv. syringae isolated from diverse woody plants in 5 to 16 days.

scholarly journals Micropropagation, Micromorphological Studies, andIn VitroFlowering inRungia pectinataL.

Scientifica ◽  
2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Mahipal S. Shekhawat ◽  
M. Manokari ◽  
C. P. Ravindran
Keyword(s):  
Tissue Culture ◽  
Shoot Multiplication ◽  
Developmental Changes ◽  
Bud Break ◽  
Ms Medium ◽  
Half Strength ◽  
Indole 3 Acetic Acid ◽  
Culture Protocol ◽  
Important Medicinal Plant

A tissue culture protocol was developed for an important medicinal plantRungia pectinataL. in the present study. Nodal shoots were used as explants and surface-sterilized with 0.1% HgCl2solution. Murashige and Skoog (MS) medium was used to establish the cultures ofR. pectinata. The bud break was reported on MS medium supplemented with 1.0 mg L−16-benzylaminopurine (BAP). About 98% response was observed with this media combination and maximum 3.2 shoots per explant with 4.3 cm length were recorded. The shoots were further multiplied using MS medium augmented with 0.5 mg L−1each of BAP and kinetin (Kin) + 0.1 mg L−1indole-3 acetic acid (IAA). Maximum 13.2 shoots per explant with 5.2 cm length were observed. All the shoots were rooted (4.9 roots per shoot with 3.5 cm length) on half strength MS medium fortified with 2.0 mg L−1indole-3 butyric acid (IBA).In vitroflowering was induced from the shoots on half strength MS medium supplemented with same concentrations and combinations of growth regulators used for shoot multiplication under 12/12 hr light/dark photoperiod. The plantlets were hardened in the greenhouse for two months and finally transferred to the field. The foliar micromorphological studies revealed the developmental changes in stomata, vein density, and trichomes during the culture of shoots underin vitroconditions.

scholarly journals Disinfection procedures for in vitro propagation of Anthurium

2015 ◽  
Vol 27 (1) ◽  
pp. 3-14 ◽  
Author(s):  
Jaime A. Teixeira da Silva ◽  
Budi Winarto ◽  
Judit Dobránszki ◽  
Songjun Zeng
Keyword(s):  
Tissue Culture ◽  
Plant Material ◽  
In Vitro Propagation ◽  
Ex Vitro ◽  
Microbial Contaminants ◽  
Culture Protocol

Abstract Disinfection of plant material is the most important step of the tissue culture protocol. In this process, an attempt is made to eliminate microbial contaminants from the surface and interior of plant material, thus giving the explant a fighting chance at survival in vitro. Initial cultures of Anthurium species and cultivars, which are usually established from ex vitro material grown in a greenhouse, pots or in the field, easily contaminate the in vitro milieu. This review highlights the differences in disinfection protocols that exist for different species or cultivars of Anthurium. The protocol needs to be adjusted based on the material used: spadices, spathes, seeds, leaves, or roots. Regrettably, most of the currently published protocols, derived from a literature that spans over 100 published papers, have numerous weaknesses and flaws in the information provided pertaining to disinfection and infection levels. Advice for future Anthurium researchers should thus be followed cautiously.

scholarly journals Standardization of tissue culture protocol for turmeric (Curcuma longa L.) Cv. Salem

2020 ◽  
Vol 8 (1) ◽  
pp. 2721-2726
Author(s):  
Sinchana NS ◽  
Kattimani KN ◽  
Prabhuling G ◽  
Sudesh K ◽  
Jagadeesha N
Keyword(s):  
Tissue Culture ◽  
Curcuma Longa ◽  
Culture Protocol
Sign in / Sign up

Export Citation Format

Share Document