Murashige and Skoog (MS) medium, a common tissue culture media for orchids which is mostly supplemented with various natural organic substances with high nutritional values can accelerate the growth of plant tissues. Nevertheless, the knowledge of actual compositions of these natural added substances is limited, causing various culture results. In this study, we have investigated the effects of added natural organic nutrient ratios on 2 orchid tissue cultures, Dendrobium, Dendrobium farmeri Paxt. and Dendrobium griffithianum Lindl. Additionally, simple, affordable medium recipes, MS media supplemented with banana were thus economically sterilized by either a simple type steaming vapor boiler or an autoclave for reliability improvement. The results showed that the orchids grew better in medium supplemented with Namwa banana especially at 150 g/L for Dendrobiumfarmeri Paxt. and 75 g/L for Dendrobium griffithianum Lindl. The suitable steaming procedure at 100°C for 60 minutes was adequate for eliminating most pathogens from the media and helped both orchid seedlings cultivated very well. Moreover, the optimized homemade tissue culture protocol provides fast effectiveness, simplicity, consistently high nutritional values, and practically being suitable technique for ready transferring to the community.
Efficient one-step tissue culture protocol for propagation of endemic plant, Lilium martagon var. cattaniae Vis.
