Characterization of egg yolk immunoglobulin (IgY) against enterotoxigenic Escherichia coli and evaluation of its effects on bovine intestinal cells

The gastrointestinal ecology (GE) of piglets fed diets containing non-starch polysaccharide hydrolysis products (HP) and egg yolk antibodies against K88 fimbriae (EYA) following oral challenge with enterotoxigenic Escherichia coli K88 (ETEC) was investigated. The HP were products of incubating feedstuffs with a blend of carbohydrase enzymes. Forty, 21-d-old pigs (two pigs/pen) were assigned to four diets to give five pens per diet. The diets were: a control fed without or with 5 g kg-1 of HP and EYA either singly or in combination forming a 2 × 2 factorial arrangement. Following a 9-d adaptation period, all pigs were orally challenged with ETEC and killed at 24 and 48 h post-challenge (one pig/pen on each occasion). Feeding HP increased pre-challenge average daily gain (252 vs. 207 g d-1; P = 0.01). An interaction (P < 0.10) between EYA and HP was observed such that when fed in combination they resulted in higher ileal digesta lactic acid and cecal DM contents and lower ileal digesta ammonia. The main effects (P < 0.05) were such that pigs fed EYA-diets had shorter intestinal crypt whilst pigs fed HP-diets showed low gastric pH and high ileal mucosal adherent lactobacilli counts. In conclusion, HP and EYA influenced indices of fermentative characteristics and intestinal morphology in the gastrointestinal ecology of piglets orally challenged with enterotoxigenic E. coli (k88).Key words: Egg yolk antibodies, ETEC, gastrointestinal ecology, non-starch polysaccharides hydrolysis products, piglet

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Characterization of the F41 fimbrial antigen of enterotoxigenic Escherichia coli by using monoclonal antibodies.

Infection and Immunity ◽

10.1128/iai.57.4.1192-1199.1989 ◽

1989 ◽

Vol 57 (4) ◽

pp. 1192-1199 ◽

Cited By ~ 10

Author(s):

F G van Zijderveld ◽

F Westenbrink ◽

J Anakotta ◽

R A Brouwers ◽

A M van Zijderveld

Keyword(s):

Escherichia Coli ◽

Monoclonal Antibodies ◽

Enterotoxigenic Escherichia Coli

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Extended-spectrum β-lactamase prevalence and virulence factor characterization of enterotoxigenic Escherichia coli responsible for acute diarrhea in Nepal from 2001 to 2016

Antimicrobial Resistance and Infection Control ◽

10.1186/s13756-018-0377-2 ◽

2018 ◽

Vol 7 (1) ◽

Cited By ~ 5

Author(s):

Katie R. Margulieux ◽

Apichai Srijan ◽

Sirigade Ruekit ◽

Panida Nobthai ◽

Kamonporn Poramathikul ◽

...

Keyword(s):

Escherichia Coli ◽

Virulence Factor ◽

Acute Diarrhea ◽

Enterotoxigenic Escherichia Coli ◽

Extended Spectrum

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Detection and characterization of the coli surface antigen 6 of enterotoxigenic Escherichia coli strains by using monoclonal antibodies.

Journal of Clinical Microbiology ◽

10.1128/jcm.35.4.867-872.1997 ◽

1997 ◽

Vol 35 (4) ◽

pp. 867-872 ◽

Cited By ~ 13

Author(s):

A Helander ◽

H M Grewal ◽

W Gaastra ◽

A M Svennerholm

Keyword(s):

Escherichia Coli ◽

Monoclonal Antibodies ◽

Surface Antigen ◽

Enterotoxigenic Escherichia Coli

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Further characterization of Escherichia coli O153:H45, an ETEC serotype disseminated in Chile

Canadian Journal of Microbiology ◽

10.1139/m88-017 ◽

1988 ◽

Vol 34 (1) ◽

pp. 85-88 ◽

Cited By ~ 2

Author(s):

M. Elena Fernández-Beroš ◽

Vincent Kissel ◽

Maria E. Agüero ◽

Guillermo Figueroa ◽

Karen D'Ottone ◽

...

Keyword(s):

Escherichia Coli ◽

Antibiotic Resistance ◽

Enterotoxigenic Escherichia Coli ◽

Plasmid Content ◽

Enterotoxin Genes ◽

Metabolic Properties ◽

Factor Antigen

The newly described stable enterotoxin producing, enterotoxigenic Escherichia coli, serotype O153:H45, capable of expressing colonizing factor antigen I, is frequently isolated as a cause of diarrhea among Chilean children. Hybridization studies of five new strains confirmed previous results which indicated that the stable enterotoxin genes are contained in nonconjugative plasmids ranging in size from 81 to 87 kilobases. The strains expressed similar antibiotic resistance and metabolic properties but differed in their plasmid content.

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