Characterization of specific egg yolk immunoglobulin (IgY) against mastitis-causing Escherichia coli

This study was selected to find out the bacterial pathogens in egg yolk, egg shell, feed and air samples of poultry houses at Dinajpur district in Bangladesh with isolation, identification and characterization of bacterial pathogens present in those samples. For this study, a total of 147 samples comprising egg shell (36), egg yolk (36), feed (45) and air (30) were collected during the period from January to May, 2012 and the collected samples were then examined for the bacteriological study by using cultural, morphological and biochemical techniques. On the basis of their cultural, morphological and biochemical properties the isolated organisms were identified as Escherichia coli, Staphylococcus spp., Salmonella serovars and Bacillus spp. In this study it was observed that out of 147 samples a total of 51 were identified as bacterial pathogens in which egg shell containing 10 (27.78%), egg yolk 11 (30.56%), feed 20 (44.44%) and air 10 (33.33%) respectively. In this study it was also observed that the highest prevalence of bacterial pathogens in feed samples (44.44%) in comparison with egg shell (27.78%), egg yolk (30.56%) and air samples (33.33%). In this study it was demonstrated that out of four (04) pathogens Escherichia coli was more abundant (39.21%) in the layer house and its environment in comparison with Staphylococcus spp. (25.49%), Salmonella (23.52%) and Bacillus spp. (11.76%) respectively.Asian J. Med. Biol. Res. June 2017, 3(2): 168-174

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Characterization of specific egg yolk immunoglobulin (IgY) against mastitis-causingStaphylococcus aureus

Journal of Applied Microbiology ◽

10.1111/j.1365-2672.2008.03920.x ◽

2008 ◽

Vol 105 (5) ◽

pp. 1529-1535 ◽

Cited By ~ 22

Author(s):

Y.-H. Zhen ◽

L.-J. Jin ◽

J. Guo ◽

X.-Y. Li ◽

Z. Li ◽

...

Keyword(s):

Egg Yolk ◽

Egg Yolk Immunoglobulin

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Preparation and Characterization of New Liposomes. Bactericidal Activity of Cefepime Encapsulated into Cationic Liposomes

Pharmaceutics ◽

10.3390/pharmaceutics11020069 ◽

2019 ◽

Vol 11 (2) ◽

pp. 69 ◽

Cited By ~ 12

Author(s):

Maria Luisa Moyá ◽

Manuel López-López ◽

José Antonio Lebrón ◽

Francisco José Ostos ◽

David Pérez ◽

...

Keyword(s):

Escherichia Coli ◽

Rate Constant ◽

Bactericidal Activity ◽

Bacterial Infections ◽

Agar Plate ◽

Egg Yolk ◽

Cationic Liposomes ◽

Growth Rate Constant ◽

E Coli

Cefepime is an antibiotic with a broad spectrum of antimicrobial activity. However, this antibiotic has several side effects and a high degradation rate. For this reason, the preparation and characterization of new liposomes that are able to encapsulate this antibiotic seem to be an important research line in the pharmaceutical industry. Anionic and cationic liposomes were prepared and characterized. All cationic structures contained the same cationic surfactant, N,N,N-triethyl-N-(12-naphthoxydodecyl)ammonium. Results showed a better encapsulation-efficiency percentage (EE%) of cefepime in liposomes with phosphatidylcholine and cholesterol than with 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE). The presence of cholesterol and the quantity of egg-yolk phospholipid in the liposome increased the encapsulation percentage. The bactericidal activity against Escherichia coli of cefepime loaded into liposomes with phosphatidylcholine was measured. The inhibitory zone in an agar plate for free cefepime was similar to that obtained for loaded cefepime. The growth-rate constant of E. coli culture was also measured in working conditions. The liposome without any antibiotic exerted no influence in such a rate constant. All obtained results suggest that PC:CH:12NBr liposomes are biocompatible nanocarriers of cefepime that can be used in bacterial infections against Escherichia coli with high inhibitory activity.

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