scholarly journals Substrate specificity of uridine and purine nucleoside phosphorylases of the Escherichia coli whole cells

1988 ◽  
Vol 4 (6) ◽  
pp. 298-302
Author(s):  
A. I. Zintchenko ◽  
L. A. Eroshevskaya ◽  
V. N. Barai ◽  
I. A. Mikhailopulo
Keyword(s):  
Escherichia Coli ◽  
Substrate Specificity ◽  
Purine Nucleoside ◽  
Whole Cells ◽  
Nucleoside Phosphorylases

Synthesis of γ-L-Glutamylalkylamides Catalyzed by Escherichia coli Having γ-Glutamyltranspeptidase Activity

2012 ◽  
Vol 550-553 ◽  
pp. 1177-1181
Author(s):  
Fei Zhang ◽  
Yin Liu ◽  
Pei Xin He
Keyword(s):  
Escherichia Coli ◽  
Substrate Specificity ◽  
Conversion Rate ◽  
Medicinal Value

γ-L-glutamyl alkylamines have potential medicinal value. In this research, five different γ-L-glutamyl alkylamines were synthesized. The result showed that substrate specificity of alkylamines, from high to low, is methylamine, ethylamine, n-propylamine, isopropylamine and n-butylamine. The reaction was optimal at pH 9.5 and 45°C, and the optimal substrate mole ratio of L-glutamine to alkylamine was 1:3 (mol/mol).Under these conditions, conversion rate of L-glutamine is about 90% (mol/mol)．

Sign in / Sign up

Export Citation Format

Share Document