scholarly journals Peer Review #1 of "Impact of copper toxicity on stone-head cabbage (Brassica oleracea var. capitata) in hydroponics (v0.1)"

2015 ◽  
Author(s):  
Sajid Ali ◽  
Muhammad Shahbaz ◽  
Ahmad Naeem Shahzad ◽  
Ammara Fatima ◽  
Hafiz Azhar Ali Khan ◽  
...  

Arable soils are frequently subjected to contamination with copper as the consequence of imbalanced fertilization with manure and organic fertilizers and/or extensive use of copper-containing fungicides. In the present study, the exposure of Stone-head cabbage (Brassica oleracea Var. capitata) to elevated Cu2+ levels resulted in leaf chlorosis and lesser biomass yield at ≥ 2 µM. Root nitrate content was not statistically affected by Cu2+ levels, although it was substantially decreased at ≥ 5 µM Cu2+ in the shoot. The decrease in nitrate contents can be related to lower nitrate uptake rates because of growth inhibition by Cu-toxicity. Shoot sulfate content increased strongly at ≥ 2 µM Cu2+ indicating an increase in demand for sulfur under Cu stress. Furthermore, at ≥ 2 µM concentration, concentration of water-soluble non-protein thiol increased markedly in the roots and to a smaller level in the shoot. When exposed to elevated concentrations of Cu2+ the improved sulfate and water-soluble non-protein thiols need further studies for the evaluation of their direct relation with the synthesis of metal-chelating compounds (i.e. phytochelatins).


2015 ◽  
Author(s):  
Sajid Ali ◽  
Muhammad Shahbaz ◽  
Ahmad Naeem Shahzad ◽  
Ammara Fatima ◽  
Hafiz Azhar Ali Khan ◽  
...  

Arable soils are frequently subjected to contamination with copper as the consequence of imbalanced fertilization with manure and organic fertilizers and/or extensive use of copper-containing fungicides. In the present study, the exposure of Stone-head cabbage (Brassica oleracea Var. capitata) to elevated Cu2+ levels resulted in leaf chlorosis and lesser biomass yield at ≥ 2 µM. Root nitrate content was not statistically affected by Cu2+ levels, although it was substantially decreased at ≥ 5 µM Cu2+ in the shoot. The decrease in nitrate contents can be related to lower nitrate uptake rates because of growth inhibition by Cu-toxicity. Shoot sulfate content increased strongly at ≥ 2 µM Cu2+ indicating an increase in demand for sulfur under Cu stress. Furthermore, at ≥ 2 µM concentration, concentration of water-soluble non-protein thiol increased markedly in the roots and to a smaller level in the shoot. When exposed to elevated concentrations of Cu2+ the improved sulfate and water-soluble non-protein thiols need further studies for the evaluation of their direct relation with the synthesis of metal-chelating compounds (i.e. phytochelatins).


2007 ◽  
Vol 67 (1) ◽  
Author(s):  
Krystyna Górecka ◽  
Dorota Krzyżanowska ◽  
Urszula Kowalska

2018 ◽  
pp. 9-12 ◽  
Author(s):  
A. S. Domblides ◽  
E. A. Domblides ◽  
L. L. Bondareva ◽  
V. F. Pivovarov

It is important to reveal the genetic base of breeding genetic material used for development of new breeding accessions among diverse Brassica oleracea L. (CC, 2n = 18). Traditional varieties, hybrids and new ones recently developed are the main genetic resources. Classification of a collection with DNA markers enables to reveal valuable genotypes and establish the breeding accession pedigree that allows developing the new accessions with sustainable economically valuable traits. The use of microsatellite markers (SSR) in B. oleracea L. has shown high efficiency in discovering genetic polymorphism between varieties and within varieties as well. In this study, 16 primer pairs have been taken to amplify microsatellite loci of genomic DNA in national 24 breeding accessions of cabbage. On the basis of the data obtained the dendrogram has been constructed with use of Jaccard’s coefficient. All loci studied were high informative, where 14 out of 16 had a PIC > 0.5. As a result, the level of genetic polymorphism has reached 85.7%. The large cluster of head cabbages consists of three subclusters: mid-maturing and early-maturing accessions of white head cabbage, red head and savoy cabbages, late-maturing and midmaturing accessions of white head cabbage, respectively. Maximum genetic distance in the cluster of head cabbages was obtained between head cabbage ‘Slava 231’ and ‘Vertu 1340’ with genetic similarity 44.7%. The two varieties of red head cabbage ‘Gako 741’ and ‘Kamennaya Golovka 447’ were genetically similar at 71.1%. The relatively low genetic similarity of these varieties can be explained by that they belong to different varietal groups. The most genetically closest varieties were ‘Zimovka 1474’ and ‘Podarok‘ with genetic similarity 86,5%. Information on topologic differentiation obtained from cluster analysis can be the basis for selection of genetically valuable breeding material with the use of DNA markers (Marker Assisted Selection).


Author(s):  
Mehtap ÖZER ◽  
Hayati KAR ◽  
Şenay MURAT DOĞRU ◽  
Nur KOBAL BEKAR ◽  
Onur KARAAĞAÇ

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