Cucumber breeding lines are a prospective material for creating new varieties for open ground in southern Russia

The varieties and hybrids of cucumbers cultivated in the Astrakhan region are insufficiently productive and are largely affected by a number of diseases. The introduction of innovative developments into production, in particular, new varieties and hybrids of domestic selection, largely solves the problem of import substitution and food security. The goal is to use promising breeding lines and their offspring as genetic sources and donors when creating new varieties and hybrids for open field conditions in southern Russia.Methods. Experiments were carried out in the fields of Precaspian agrarian federal scientific center of the Russian academy of sciences, according to the methods used in the Astrakhan region. Within three years, 47 collection samples were tested.Results. The article presents the results of studying the 12 most promising ones, distinguished by a complex of valuable economic traits, in particular by yield, amicability of fruit setting, which are of interest for further breeding work. According to the results of the data obtained, we can say that the best were the lines: 13-88RZ, 13-101RZ, Gherkins, KRASTAVAC, Courage, Zina, they can be used to create new varieties and hybrids of cucumber as donors of a predominantly female type of flowering, harmonious yield.

Evaluation of onion samples (Allium cepa L.) of various origins in the foothill zone of the North Caucasus and creation of source material fo

Relevance. The success of breeding work is largely determined by the source material, the search and creation of which for target breeding begins with the collection and study of collectible samples for the alignment of morphological features; for the precocity and amity of maturation; for the keeping quality and transportability of bulbs; for the quality of vegetable products (high content of biologically active substances and antioxidants); for resistance to biotic and abiotic stressors of a specific growing zone.Materials and methods. On the basis of the North Caucasus branch FSBSI FSVC, 90 samples of spring onions from 26 countries of the world were evaluated in the number nursery for the period from 2017 to 2019, 71 of them were varieties and 19 were F1 hybrids. In a hybrid nursery, 25 hybrid combinations obtained from FSBSI FSVC (VNIISSOK) were evaluated. The laying and placement of experiments, agricultural techniques of cultivation, accounting and evaluation of economically valuable traits, biochemical analyses were carried out according to generally accepted methods. The standards are the new Primo and Ampex varieties of the selection of the FSBSI FSVC and the Italian F1 hybrid Ranko, which were placed every 10 collection samples.Results.According to the results of the assessment, among the studied F1 varieties and hybrids, sources of economically valuable traits such as yield (20-27 t/ha), precocity (80-90 days), preservation (more than 85%) when laid for long-term storage up to 7 months, purple, white and pink color of bulbs, as well as sources of long-shaped bulbs were identified. The greatest interest for breeding for yield is represented by 9 promising varieties and 9 F1 hybrids of onions of various origins, as well as 4 hybrid combinations that were distinguished by high yields, the yield of marketable products and the mass of marketable bulbs. These samples are recommended for use in the breeding process as sources for the creation of new varieties and hybrids with high yields for the agro-climatic conditions of the foothill zone of the North Caucasus.r breeding

Creation of new tomato forms with fungal disease resistance genes based on marker selection

Relevance. The presented studies are aimed at obtaining new forms of tomato with a complex of genes for resistance to fungal diseases in combination with a standard type of bush and dark coloring of fruits based on marker-mediated selection.Methodology. The biological objects of the study are varieties and hybrid forms of tomato from the collection of the Michurinsky SAU. Molecular genetic analysis was performed using the following methods. DNA extraction was carried out from young leaves using a kit for isolation of NC Sample NC manufactured by Agrodiagnostika LLC according to the manufacturer's protocol. Fermentas production kits were used for PCR. Identification of the cladosporosis resistance gene was Cf-19 performed using the DNA marker R7. The presence of a fusarious wilting resistance gene was determined by a I-2/5 marker. The amplification results were visualized by agarose gel electrophoresis.Results. During the research, a collection of varieties and hybrid forms of tomato of the Michurinsky GAU was analyzed in order to identify genes for resistance to cladosporiosis Cf-19 and fusarium wilt I-2. A total of 52 genotypes were analyzed. It was found that most samples (41 samples) are characterized by a heterozygous state of the Cf-19 gene. All indeterminant and semi-determinant forms had both alleles. Of the 23 determinant forms presented in the collection, 10 had only one allele corresponding to recessive homozygote. Among all analyzed tomato genotypes, no dominant homozygous forms were noted. The study of the collection revealed several alleles of the I-2 gene. In total, four fragments corresponding to various alleles were amplified. A total of 50 resistant genotypes have been identified in the collection. Two alleys of the I-2 gene (633/693 bp) were identified in 42 tomato samples. Four varieties are homozygous in one allele (633 bp), which determines resistance. Three varieties have a second resistance allele (566 bp). One genotype has only an allele defining susceptibility (693 bp). On the basis of molecular analysis, as well as an assessment of the type of bush and fetal color, initial forms were selected with subsequent hybridization. 67 hybrid tomato plants were obtained. Evaluation of the presence of resistance genes showed that most of the resulting hybrids are resistant to cladosporiosis and fuzariosis. This is due to the presence of dominant alleles of Cf-19 and I-2 genes in a heterozygous state. Among the resulting hybrids, plants with a bark type of bush were identified. A total of 13 such plants were obtained.Conclusion. Thus, the work carried out allowed to obtain hybrid forms of tomato combine the signs of resistance to two pathogens of fungal diseases and the stem type of the bush. These forms are planned to be used in further selection work.

Creation of competitive varieties of melon and pumpkin with valuable economic trends

Relevance. The soil and climatic conditions of the Lower Trans-Volga region are considered favorable for melon growing and make it possible to obtain high-quality products. The purpose of the research was to create new competitive varieties and hybrids of melon and pumpkin.Materials and methods. The object of the research is new varieties of melon and butternut pumpkin created at the Bykovskaya melon breeding experimental station. Melon cultivar Osen and pumpkin cv. Zhemchuzhina were used as standards. The methods of creation are intervarietal hybridization, individual and mass selection. During the growing season, appropriate observations and counts were carried out.Results. As a result of many years of breeding work at the Bykovskaya cucurbits selection experimental station, a melon cultivar 251 and a pumpkin cultivar 509 have been created. both producers and consumers. Therefore, the obtained varieties were evaluated for taste, yield, resistance to biological and abiofactors of the environment, dry matter content, fruit size, fruit and pulp color. During the comparative assessment of the accessions, it was determined that the new varieties of melon and pumpkin exceed the standards in terms of the main economically valuable traits. The average yield during the study, in the melon cultivar 251, exceeded the standard by 47.8%. In the pumpkin cultivar, the average yield for three years of research exceeded the standard by 69.6%. In terms of taste, the new melon variety was at the level of the standard, the cv. Osen. A variety of butternut pumpkin exceeded the standard in all quality indicators. The susceptibility to powdery mildew in the melon cultivar varied depending on the year of research. The melon sample showed resistance to anthracnose higher than the standard by 16.4 and 18.6%, over the years of study. In the pumpkin cultivar, resistance to powdery mildew and anthracnose was higher than the standard, on average by 34.9% to powdery mildew and by 28.6% to anthracnose. Thus, new varieties of melon and pumpkin meet the modern requirements of the industrial melon industry, are resistant to environmental stress factors, common diseases, and have economically valuable traits.

Formation of plants density and seed yield of soybean varieties in Altai Krai

Relevance. Soybean (Glycine max (L.) Merrill is one of the most important protein- oilseed crops in world arable farming. An acute shortage of both food and feed protein is felt in many world’s countries. In this regard, the expansion of soybean seed production is relevant and is dictated by the need for import substitution of both food and feed soybean products.Materials and methods. In the research we studied the influence of two ecologically different cultivation zones: Priobskaya and Prialtaiskaya on the formation of plants density and seed yield of soybean. Three varieties Altom, Gratsia and Pripyat were taken as the objects of the research.Results. On average, over the years of research in full seedlings stage from 60 germinating seeds per square meter (600 thousand pieces/ha), in our experiment, about 56 plants were obtained per 1 m2 or 560 thousand plants per 1 hectare. This means that, on average, 93% of sown seeds give seedlings in field conditions. For harvesting after exposure of soybean crops to numerous biotic and abiotic factors, on average, there are about 52 plants per 1 m² or 520 thousand plants per 1 hectare, that is, 88% of the sown germinating seeds are saved for harvesting and give a yield. The maximum influence on the variability of plant density is exerted by vegetation conditions (years) – 37%. On average, over the years of the study, the maximum yield under Topchikha conditions was obtained in 2019 – 1.9 t/ha – in a year that was distinguished by not hot weather with sufficient rainfall during the growing season. Varieties Gratsia and Pripyat in Smolenskoye conditions significantly exceeded the standard in terms of yield in 2018 and 2020. On average, over the years of research, the Gratsia variety showed itself as more stable with Cv=11.3% in Topchikha conditions and Cv=9.8% in Smolenskoye conditions.

Early diagnosis of cucumber plants resistance to low light

Relevance. When growing cucumbers in a greenhouse not equipped with additional lighting, especially in winter, there is a shortage of solar energy. To obtain high yields of cucumber at this time, it is necessary to select the most resistant to low light hybrids. The selection of genotypes resistant to this trait is a very important stage in the selection of cucumber for winter-spring turnover.The purpose of the study: search for an easy-to-use method for early diagnosis of cucumber resistance to insufficient illumination.Methods. The research was carried out at the variety testing site in the Gavrish Breeding Center (Krymsk city) of the Scientific Research Institute of Vegetable Crop Breeding, in winter-spring turnover conditions, in greenhouses not equipped with artificial lighting. Seedlings were planted in a permanent place in the greenhouse during the period of the lowest arrival of solar radiation in the year on December 21. The measurement of the height of the main stem was carried out on 31 days after planting the plants in a permanent place.Results. Measuring the height of the main stem before fruiting showed a significant variation of this trait in different cucumber hybrids. Further study of the correlation of the height of the main stem and yield revealed their high dependence. This fact suggests that a fairly simple measurable parameter has been found for evaluating different cucumber genotypes on the basis of resistance to low light. Regression analysis made it possible to divide the variability of the studied genotypes by yield into a dependent part of the height of the main stem and an additional one, which makes up to 30% of the yield increase. Further analysis of the high-yielding group has a high breeding value.

Prospective varieties of dahlia cultural (Dahlia x cultorum Thorsr. et Reis.) when growing for shearing in conditions of protected ground

Relevance. In terms of the richness of colors, shapes and sizes of inflorescences and relative unpretentiousness during cultivation, as well as the duration of flowering of a dahlia, the cultural has no equal. Basically, this flower crop is grown in the open field and used for landscaping areas. Dahlias, due to their decorative properties, are a promising crop for cutting. But the growth in the volume of industrial cultivation of cut dahlias has long been constrained by two negative factors - the low life expectancy of cut flowers, and the fact that not all varieties of cultivated dahlias are suitable for cultivation in greenhouses. Therefore, the search for varieties that meet the aforementioned properties is an especially urgent task.Material and methods. The material for the study was 10 varieties of Dahlia × cultorum Dutch selection. They belong to different groups of dahlias – spherical: Souvenir d'Eté, The Voice, Jowey Winnie; decorative – Orion, Café au Lait, Fleurel, Karma Choc and pompom dahlias: Little William, Cornel Bronze, Petra's Wedding, All tubers were planted in phytotron beds. Phenological observations of plants were carried out according to the Methodology of State Variety Testing of Ornamental Crops. During the growing season, the following indicators were determined: the onset of phenological phases of development, the dynamics of plant growth, the number and size of inflorescences, the length of the peduncle, the duration of flowering.Results. It was found that all the studied varieties of cultural dahlias have a high decorative effect and are suitable for growing for cutting in greenhouse conditions. Growing plants in a phytotron allows you to get a high-quality cut almost two months ahead of time, as well as have a longer and more abundant flowering. It was found that the Café au Lait cultivar is not entirely suitable for cut cultivation in greenhouses or requires the development of cultivation technology that is individual for this cultivar. It was found that the Fleurel variety surpasses other studied varieties in terms of decorativeness, inflorescence size, peduncle length, onset time and flowering duration. A significant disadvantage of the variety is its high growth and fragility of the peduncles. The most popular cut varieties were Fleurel, Petra's Wedding, Jowey Winnie and Little William. It was found that the studied varieties of cultural dahlias were distinguished not only by high decorativeness, but also by high durability in a vase from 5 to 7 days.

Study of Phytophthora infestans Mont. de Bary isolates in the planting of potatoes

Relevance. One of the most common diseases of potatoes and other nightshade family species is late blight caused by a pathogenic oomycete of the Phytophthora infestans (Mont.) de Bary. At least 100 species of phytophthora have been described in nature, affecting a wide range of plant species. The phytophthora population is heterogeneous and is represented by races, as well as different types of mating. This leads to a rapid adaptation of the pathogen and the emergence of new, more aggressive, and resistant races. Phytophthora is a parasite, the damage from which cannot be avoided within the organic farming framework. Therefore, it is particularly important to know the pathogenesis and racial composition of phytophthora in each individual region of Solanaceae cultivation.Research methodology. Differentiation and collection of material from the natural population were carried out using potato varieties with known R-genes in the genome. Isolation and introduction into the culture were carried out from leaves with the dampening chambers method, followed by cultivation on nutrient media. The pathogen was identified by microscopic analysis. Culture filtrates were obtained on the liquid nutritious medium, followed by liquid filtration and autoclaving. Phytotoxic activity was determined by the effect on the seedlings of the nightshade, grass, and pea families by the standard method. Molecular genetic analysis of the isolates was carried out by ISSR analysis; the primer, amplification mixture, and temperature profile of the reaction were selected according to the literature data; the calculation of genetic characteristics was carried out using POPGENE software packages.Results. Samples of seven Phytophthora infestans isolates were collected and introduced into culture. As a result of in vitro cultivation, morphological differences were revealed, expressed in the structure and color of the mycelium, the shape of the colonies, the nature of sporulation, the color of the reverse, and the medium under the colonies. The genetic differences of the natural phytophthora material introduced into the culture, collected from potato varieties with single resistance genes (R1, R3, R4), were revealed. Differences in the phytotoxic activity of the studied isolates' cultural filtrates were revealed. The isolated isolates demonstrate differentiation at the phenotypic, genetic and physiological levels, which allows us to speak about their belonging to races.

Morphometric trait manifestation in inbred lines of monogerm beetroot (Beta vulgaris L.)

Relevance. Inbreeding is the most widespread method of obtaining starting breeding material and plays a central role in heterosis breeding programs of most crops. Beetroot inbred lines possessing economically valuable characteristics require constant maintenance via self-pollination. However, self-pollination leads to depression of a number of traits. Thorough theoretical studies and practical developments are needed to preserve valuable beetroot lines. We aimed to study the root and leaf rosette variability resulting from beetroot self-pollination. We also investigated the inheritance of monogermity in beetroots.Materials and methods. Experimental work was carried out at the All-Russian Research Institute of Vegetable Growing – branch of the Federal State Budgetary Scientific Institution "Federal Scientific Vegetable Center" (FSBSI FSVC), 2011-202], Moscow Region. The object of the study was inbred beetroot lines originated from beetroots of foreign selection. The research data were obtained via field observations and measurements.Results. It has been experimentally shown that the constant signs of self-pollinated beet lines of 1 year of life are the position and height of the leaf rosette. Stabilization and maintenance of separate fertility at a high level is carried out by selection. A negative correlation was noted between the signs of the degree of separateness and the height of the leaf rosette of the plant, the proportion of the neck of the root crop in its diameter, the mass of the root crop and the proportion of the root crop in the biomass of the plant in the offspring of the fourth generation of the inbreeding. The decrease in the productivity of the seed plant occurs at the beginning of the process of creating self-pollinated lines – in the first generation.

Results of competitive variety testing of new squash varieties in Uzbekistan

The aim of the research is to breed short climbing, early maturing, high-yielding varieties of squash in the natural and climatic zone of Uzbekistan.Methods. The samples of squash 0044SQ (Holland) and BT+KB-001 (Turkey) were taken from the gene pool of the Research Institute of Plant Industry (Uzbekistan) in 2012 for breeding work. In 2013, squash samples were studied in the nursery of the source material, where they were evaluated for biological and economically valuable traits. Individual selection of plants was carried out. In further breeding work (2014-2016 and 2018), the lines LZ-2513 and LH-1916 were distinguished.Results. The method of analytical breeding created short-climbing, early maturing, high-yielding of squash lines LZ-2513 and LH-1916. Line LZ-2513 has rounded fruits of light green color. The LH-1916 variety has elongated, dark green fruits. Competitive variety trials of new lines were conducted in 2019-2020. Unumdor variety zoned in the republic served as a standard. It was found that from sprouting to maturity of the fruit in the line LZ-2513 is 44 days, in the line LH-1916 – 45 days. High marketable yield was recorded for squash line LZ-2513 – 18.3 tons/ha (122% of the standard); in line LH-1916 marketable yield was 16.4 tons/ha (112.4% of the standard). This year we organized the primary seed production of squash lines LZ-2513 and LH-1916 for multiplication.Conclusion. Promising for cultivation in conditions of Uzbekistan squash line LZ-2513 Orbita (NAP 20200087) and line LH-1916 Viridi (NAP 20200088) were submitted to the Intellectual Property Agency under the Ministry of Justice of the Republic of Uzbekistan to be patented.