scholarly journals Peer Review #3 of "Comparison of miRNA expression profiles in pituitary–adrenal axis between Beagle and Chinese Field dogs after chronic stress exposure (v0.1)"

2016 ◽  
Keyword(s):  
Peer Review ◽  
Chronic Stress ◽  
Mirna Expression ◽  
Expression Profiles ◽  
Stress Exposure ◽  
Adrenal Axis ◽  
Mirna Expression Profiles ◽  
Pituitary Adrenal Axis

scholarly journals Comparison of miRNA expression profiles in pituitary–adrenal axis between Beagle and Chinese Field dogs after chronic stress exposure

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e1682 ◽  
Author(s):  
Wei Luo ◽  
Meixia Fang ◽  
Haiping Xu ◽  
Huijie Xing ◽  
Jiangnan Fu ◽  
...  
Keyword(s):  
Stress Response ◽  
Adrenal Cortex ◽  
Chronic Stress ◽  
Hpa Axis ◽  
Mirna Expression ◽  
Target Genes ◽  
Expression Profiles ◽  
Stress Exposure ◽  
Mirna Expression Profiles ◽  
Pituitary Adrenal Axis

MicoRNAs (miRNAs), usually as gene regulators, participate in various biological processes, including stress responses. The hypothalamus–pituitary–adrenal axis (HPA axis) is an important pathway in regulating stress response. Although the mechanism that HPA axis regulates stress response has been basically revealed, the knowledge that miRNAs regulate stress response within HPA axis, still remains poor. The object of this study was to investigate the miRNAs in the pituitary and adrenal cortex that regulate chronic stress response with high-throughput sequencing. The pituitary and adrenal cortex of beagles and Chinese Field dogs (CFD) from a stress exposure group (including beagle pituitary 1 (BP1), CFD pituitary 1 (CFDP1), beagle adrenal cortex 1 (BAC1), CFD adrenal cortex 1 (CFDAC1)) and a control group (including beagle pituitary 2 (BP2), CFD pituitary 2 (CFDP2), beagle adrenal cortex 2 (BAC2), CFD adrenal cortex 2 (CFDAC2)), were selected for miRNA-seq comparisons. Comparisons, that were made in pituitary (including BP1 vs. BP2, CFDP1 vs. CFDP2, BP1 vs. CFDP1 and BP2 vs. CFDP2) and adrenal cortex (including BAC1 vs. BAC2, CFDAC1 vs. CFDAC2, BAC1 vs. CFDAC1 and BAC2 vs. CFDAC2), showed that a total of 39 and 18 common differentially expressed miRNAs (DE-miRNAs) (Total read counts > 1,000, Fold change > 2 &p-value < 0.001), that shared in at least two pituitary comparisons and at least two adrenal cortex comparisons, were detected separately. These identified DE-miRNAs were predicted for target genes, thus resulting in 3,959 and 4,010 target genes in pituitary and adrenal cortex, respectively. Further, 105 and 10 differentially expressed genes (DEGs) (Fold change > 2 &p-value < 0.05) from those target genes in pituitary and adrenal cortex were obtained separately, in combination with our previous corresponding transcriptome study. Meanwhile, in line with that miRNAs usually negatively regulated their target genes and the dual luciferase reporter assay, we finally identified cfa-miR-205 might play an important role by upregulatingMMDin pituitary and hippocampus, thus enhancing the immune response, under chronic stress exposure. Our results shed light on the miRNA expression profiles in the pituitary and adrenal cortex with and without chronic stress exposure, and provide a new insight into miR-205 with its feasible role in regulating chronic stress in the pituitary and hippocampus through targetingMMD.

The Hypothalamic-Pituitary-Adrenal Axis, Obesity, and Chronic Stress Exposure: Foods and HPA Axis

2012 ◽  
Vol 1 (4) ◽  
pp. 199-207 ◽  
Author(s):  
Femke Rutters ◽  
Susanne La Fleur ◽  
Sofie Lemmens ◽  
Jurriaan Born ◽  
Mieke Martens ◽  
...  
Keyword(s):  
Chronic Stress ◽  
Hpa Axis ◽  
Hypothalamic Pituitary Adrenal Axis ◽  
Stress Exposure ◽  
Hypothalamic Pituitary Adrenal ◽  
Adrenal Axis ◽  
Pituitary Adrenal Axis

Transcriptome comparison in the pituitary-adrenal axis between Beagle and Chinese Field dogs after chronic stress exposure

2015 ◽  
Vol 46 (5) ◽  
pp. 522-534 ◽  
Author(s):  
Wei Luo ◽  
Meixia Fang ◽  
Haiping Xu ◽  
Huijie Xing ◽  
Qinghua Nie
Keyword(s):  
Chronic Stress ◽  
Stress Exposure ◽  
Adrenal Axis ◽  
Transcriptome Comparison ◽  
Pituitary Adrenal Axis
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