scholarly journals Purification and characterization of tannase from the local isolate of Aspergillus niger

2019 ◽  
Vol 7 (1) ◽  
pp. 29-34 ◽  
2010 ◽  
Vol 4 (2) ◽  
pp. 78-85
Author(s):  
Jasim M. Awdaa

Inulinase was produced from local isolate of Aspergillus niger J3. The inulinase was purified by two steps included precipitation by amonium sulphate at (30-80) % sa-turation and gel filtration on sephadex G100. The final purification folds and the yield of the enzyme were 3.15 times and 28.24%, respectively. The purified enzyme has the following characteristics: The optimum pH of the enzyme activity was 5.5. The enzyme was most stable at pH (4.5 - 6). The optimum temperature for its activity was 45c. The enzyme retained its original activity when incubates at (30-55) c for 20 minutes. Mercury chloride inhibited the enzyme completely at concentration of 10mM, cupper sulphate and calisium chloride inhibited the enzyme at concentrations of 85% and 7% respectively. It was revealed that the enzyme had the efficiency to hydrolyze 87% of 5% inulin solution when treated at 45c for 120 min.


2009 ◽  
Vol 25 (12) ◽  
pp. 2095-2102 ◽  
Author(s):  
Georgi Dobrev ◽  
Boriana Zhekova ◽  
Ginka Delcheva ◽  
Lidia Koleva ◽  
Nicola Tziporkov ◽  
...  

2020 ◽  
Vol 57 (7) ◽  
pp. 2669-2680
Author(s):  
Shuqi Xing ◽  
Ruonan Zhu ◽  
Cuiqin Li ◽  
Laping He ◽  
Xuefeng Zeng ◽  
...  

2011 ◽  
Vol 11 (1) ◽  
pp. 2 ◽  
Author(s):  
Ondřej Kaplan ◽  
Karel Bezouška ◽  
Ondřej Plíhal ◽  
Rüdiger Ettrich ◽  
Natallia Kulik ◽  
...  

2013 ◽  
Vol 13 (1) ◽  
Author(s):  
Ondrej Kaplan ◽  
Karel Bezouska ◽  
Ondrej Plihal ◽  
Rudiger Ettrich ◽  
Natallia Kulik ◽  
...  

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