Partial Purification and Characterization of Cellulase (Ec.3.2.1.4) from Aspergillus niger Using Saw Dust as Carbon Source

2017 ◽  
Vol 06 (01) ◽  
Author(s):  
Ekpa Emmanuel ◽  
Adebisi Queen
Keyword(s):  
Aspergillus Niger ◽  
Carbon Source ◽  
Partial Purification ◽  
Purification And Characterization ◽  
Saw Dust

scholarly journals Partial Purification and Characterization of Inulinase Produced from a local isolate of Aspergillus niger J3

2010 ◽  
Vol 4 (2) ◽  
pp. 78-85
Author(s):  
Jasim M. Awdaa
Keyword(s):  
Aspergillus Niger ◽  
Gel Filtration ◽  
Partial Purification ◽  
Mercury Chloride ◽  
Purification And Characterization ◽  
Original Activity ◽  
Local Isolate ◽  
Optimum Ph ◽  
Final Purification

Inulinase was produced from local isolate of Aspergillus niger J3. The inulinase was purified by two steps included precipitation by amonium sulphate at (30-80) % sa-turation and gel filtration on sephadex G100. The final purification folds and the yield of the enzyme were 3.15 times and 28.24%, respectively. The purified enzyme has the following characteristics: The optimum pH of the enzyme activity was 5.5. The enzyme was most stable at pH (4.5 - 6). The optimum temperature for its activity was 45c. The enzyme retained its original activity when incubates at (30-55) c for 20 minutes. Mercury chloride inhibited the enzyme completely at concentration of 10mM, cupper sulphate and calisium chloride inhibited the enzyme at concentrations of 85% and 7% respectively. It was revealed that the enzyme had the efficiency to hydrolyze 87% of 5% inulin solution when treated at 45c for 120 min.

Partial Purification and Characterization of Sulfhydryl Oxidase from Aspergillus niger

2002 ◽  
Vol 67 (6) ◽  
pp. 2016-2022 ◽  
Author(s):  
C. Vignaud ◽  
N. kaid ◽  
L. Rakotozafy ◽  
S. Davidou ◽  
J. Nicolas
Keyword(s):  
Aspergillus Niger ◽  
Partial Purification ◽  
Sulfhydryl Oxidase ◽  
Purification And Characterization

scholarly journals Purification and characterization of endoxylanase Xln-1 from Aspergillus niger B03

2009 ◽  
Vol 25 (12) ◽  
pp. 2095-2102 ◽  
Author(s):  
Georgi Dobrev ◽  
Boriana Zhekova ◽  
Ginka Delcheva ◽  
Lidia Koleva ◽  
Nicola Tziporkov ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Purification And Characterization
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