scholarly journals The long non-coding RNA Cerox1 is a post transcriptional regulator of mitochondrial complex I catalytic activity

eLife ◽  
2019 ◽  
Vol 8 ◽  
Author(s):  
Tamara M Sirey ◽  
Kenny Roberts ◽  
Wilfried Haerty ◽  
Oscar Bedoya-Reina ◽  
Sebastian Rogatti-Granados ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Complex I ◽  
Noncoding Rna ◽  
Mitochondrial Complex I ◽  
Mitochondrial Complex ◽  
Non Coding Rna ◽  
Transport Chain ◽  
Species Production ◽  
Complex I Activity ◽  
Long Non Coding Rna

To generate energy efficiently, the cell is uniquely challenged to co-ordinate the abundance of electron transport chain protein subunits expressed from both nuclear and mitochondrial genomes. How an effective stoichiometry of this many constituent subunits is co-ordinated post-transcriptionally remains poorly understood. Here we show that Cerox1, an unusually abundant cytoplasmic long noncoding RNA (lncRNA), modulates the levels of mitochondrial complex I subunit transcripts in a manner that requires binding to microRNA-488-3p. Increased abundance of Cerox1 cooperatively elevates complex I subunit protein abundance and enzymatic activity, decreases reactive oxygen species production, and protects against the complex I inhibitor rotenone. Cerox1 function is conserved across placental mammals: human and mouse orthologues effectively modulate complex I enzymatic activity in mouse and human cells, respectively. Cerox1 is the first lncRNA demonstrated, to our knowledge, to regulate mitochondrial oxidative phosphorylation and, with miR-488-3p, represent novel targets for the modulation of complex I activity.

scholarly journals Cerox1 and microRNA-488-3p noncoding RNAs jointly regulate mitochondrial complex I catalytic activity

2018 ◽  
Author(s):  
Tamara M Sirey ◽  
Kenny Roberts ◽  
Wilfried Haerty ◽  
Oscar Bedoya-Reina ◽  
Sebastian Rogatti-Granados ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Complex I ◽  
Noncoding Rna ◽  
Mitochondrial Complex I ◽  
Mitochondrial Complex ◽  
Protein Subunits ◽  
Transport Chain ◽  
Species Production ◽  
Complex I Activity ◽  
Human And Mouse

AbstractTo generate energy efficiently, the cell is uniquely challenged to co-ordinate the abundance of electron transport chain protein subunits expressed from both nuclear and mitochondrial genomes. How an effective stoichiometry of this many constituent subunits is co-ordinated post-transcriptionally remains poorly understood. Here we show that Cerox1, an unusually abundant cytoplasmic long noncoding RNA (lncRNA), modulates the levels of mitochondrial complex I subunit transcripts in a manner that requires binding to microRNA-488-3p. Increased abundance of Cerox1 cooperatively elevates complex I subunit protein abundance and enzymatic activity, decreases reactive oxygen species production, and protects against the complex I inhibitor rotenone. Cerox1 function is conserved across placental mammals: human and mouse orthologues effectively modulate complex I enzymatic activity in mouse and human cells, respectively. Cerox1 is the first lncRNA demonstrated, to our knowledge, to regulate mitochondrial oxidative phosphorylation (OXPHOS) and, with miR-488-3p, represent novel targets for the modulation of complex I activity.

scholarly journals Correction: The long non-coding RNA Cerox1 is a post transcriptional regulator of mitochondrial complex I catalytic activity

eLife ◽  
2019 ◽  
Vol 8 ◽  
Author(s):  
Tamara M Sirey ◽  
Kenny Roberts ◽  
Wilfried Haerty ◽  
Oscar Bedoya-Reina ◽  
Sebastian Rogatti-Granados ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Complex I ◽  
Transcriptional Regulator ◽  
Mitochondrial Complex I ◽  
Mitochondrial Complex ◽  
Non Coding Rna ◽  
Long Non Coding Rna

CFM6 is an Essential CRM Protein Required for the Splicing of nad5 Transcript in Arabidopsis Mitochondria

2021 ◽  
Author(s):  
Wei-Chih Lin ◽  
Ya-Huei Chen ◽  
Shin-Yuan Gu ◽  
Hwei-Ling Shen ◽  
Kai-Chau Huang ◽  
...  
Keyword(s):  
Complex I ◽  
Mitochondrial Complex I ◽  
Mitochondrial Complex ◽  
High Accumulation ◽  
Stunted Growth ◽  
Growth Defects ◽  
Group I ◽  
Or Genes ◽  
Complex I Activity ◽  
Intron 4

Abstract Plant CRM domain-containing proteins are capable of binding RNA to facilitate the splicing of group I or II introns in chloroplasts, but their functions in mitochondria are less clear. In the present study, Arabidopsis thaliana CFM6, a protein with a single CRM domain, was expressed in most plant tissues, particularly in flower tissues, and restricted to mitochondria. Mutation of CFM6 causes severe growth defects, including stunted growth, curled leaves, delayed embryogenesis, and pollen development. CFM6 functions specifically in the splicing of group II intron 4 of nad5, which encodes a subunit of mitochondrial complex I, as evidenced by the loss of nad5 intron 4 splicing and high accumulation of its pretranscripts in cfm6 mutants. The phenotypic and splicing defects of cfm6 were rescued in transgenic plants overexpressing 35S::CFM6-YFP. Splicing failure in cfm6 also led to the loss of complex I activity and to its improper assembly. Moreover, dysfunction of complex I induced the expression of proteins or genes involved in alternative respiratory pathways in cfm6. Collectively, CFM6, a previously uncharacterized CRM domain-containing protein, is specifically involved in the cis-splicing of nad5 intron 4 and plays a pivotal role in mitochondrial complex I biogenesis and normal plant growth.

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