scholarly journals METAGENOMIC NEXT-GENERATION SEQUENCING REVEALS MIAMIENSIS AVIDUS (CILIOPHORA: SCUTICOCILIATIDA) IN THE 2017 EPIZOOTIC OF LEOPARD SHARKS (TRIAKIS SEMIFASCIATA) IN SAN FRANCISCO BAY, CALIFORNIA, USA

2019 ◽  
Vol 55 (2) ◽  
pp. 375 ◽  
Author(s):  
Hanna Retallack ◽  
Mark S. Okihiro ◽  
Elliot Britton ◽  
Sean Van Sommeran ◽  
Joseph L. DeRisi
Keyword(s):  
Next Generation Sequencing ◽  
San Francisco ◽  
San Francisco Bay ◽  
Next Generation ◽  
Miamiensis Avidus ◽  
Triakis Semifasciata ◽  
Leopard Sharks ◽  
Generation Sequencing

scholarly journals Metagenomic next-generation sequencing reveals Miamiensis avidus (Ciliophora: Scuticociliatida) in the 2017 epizootic of leopard sharks (Triakis semifasciata) in San Francisco Bay, California

2018 ◽  
Author(s):  
Hanna Retallack ◽  
Mark S. Okihiro ◽  
Elliot Britton ◽  
Sean Van Sommeran ◽  
Joseph L. DeRisi
Keyword(s):  
Next Generation Sequencing ◽  
San Francisco ◽  
San Francisco Bay ◽  
Metagenomic Sequencing ◽  
Next Generation ◽  
Ciliated Protozoan ◽  
Miamiensis Avidus ◽  
Triakis Semifasciata ◽  
Leopard Sharks ◽  
Generation Sequencing

ABSTRACTDuring March to August of 2017, hundreds of leopard sharks (Triakis semifasciata) stranded and died on the shores of San Francisco Bay, California, USA. Similar mass stranding events occurred in 1967 and 2011, yet analysis of these epizootics was incomplete and no etiology was confirmed. Our investigation of the most recent epizootic revealed severe meningoencephalitis in stranded sharks, raising suspicion for infection. On this basis, we pursued a strategy for unbiased pathogen detection using metagenomic next-generation sequencing followed by orthogonal validation and further screening. We show that the ciliated protozoan pathogen, Miamiensis avidus, was present in the central nervous system of leopard (n=12) and other shark species (n=2) that stranded in San Francisco Bay, but absent in leopard sharks caught elsewhere. Whereas this protozoan has previously been implicated in devastating outbreaks in teleost marine fish, our findings represent the first report of a ciliated protozoan infection in wild elasmobranchs. This discovery highlights the benefits of adopting unbiased metagenomic sequencing in the study of wildlife health and disease.

Next-Generation Sequencing

2012 ◽  
Vol 42 (9) ◽  
pp. 8
Author(s):  
PETER HULICK
Keyword(s):  
Next Generation Sequencing ◽  
Next Generation ◽  
Generation Sequencing

Labordiagnostik bei gastrointestinalen Tumoren

2020 ◽  
Vol 11 (05) ◽  
pp. 232-238
Author(s):  
Marcus Kleber
Keyword(s):  
Next Generation Sequencing ◽  
Kolorektales Karzinom ◽  
Next Generation ◽  
Generation Sequencing

ZUSAMMENFASSUNGDas kolorektale Karzinom (KRK) ist einer der häufigsten malignen Tumoren in Deutschland. Einer frühzeitigen Diagnostik kommt große Bedeutung zu. Goldstandard ist hier die Koloskopie. Die aktuelle S3-Leitlinie Kolorektales Karzinom empfiehlt zum KRK-Screening den fäkalen okkulten Bluttest. Für das Monitoring von Patienten vor und nach Tumorresektion werden die Messung des Carcinoembryonalen Antigens (CEA) und der Mikrosatellitenstabilität empfohlen. Für die Auswahl der korrekten Chemotherapie scheint derzeit eine Überprüfung des Mutationsstatus, mindestens des KRAS-Gens und des BRAF-Gens, sinnvoll zu sein. Eine Reihe an neuartigen Tumormarkern befindet sich momentan in der Entwicklung, hat jedoch noch nicht die Reife für eine mögliche Anwendung in der Routinediagnostik erreicht. Den schnellsten Weg in die breite Anwendung können Next-Generation-Sequencing-basierte genetische Tests finden.

Darstellung einer verminderten T-Zellrezeptorvariabilität bei refraktärer Zöliakie Typ II mittels Next Generation Sequencing

2014 ◽  
Vol 52 (08) ◽  
Author(s):  
M Schumann ◽  
J Ritter ◽  
K Zimmermann ◽  
JD Schulzke ◽  
B Siegmund ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Next Generation ◽  
Generation Sequencing
Sign in / Sign up

Export Citation Format

Share Document