scholarly journals B04 Preliminary test of blood meal identification by enzyme-linked immunosorbent assay (ELISA) and gel diffusion on Culex tritaeniorhynchus

2003 ◽  
Vol 54 (supplement) ◽  
pp. 34
Author(s):  
M. Hasegawa ◽  
Y. Nguyen Thi ◽  
P. Tran Vu ◽  
T. Yanagi ◽  
M. Takagi
Keyword(s):  
Immunosorbent Assay ◽  
Blood Meal ◽  
Preliminary Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Culex Tritaeniorhynchus ◽  
Gel Diffusion ◽  
Blood Meal Identification

Comparison of indirect haemagglutination test, gel-diffusion precipitin test, and enzyme-linked immunosorbent assay for detection of serum antibodies to Pasteurella multocida in naturally and experimentally infected rabbits

1994 ◽  
Vol 28 (1) ◽  
pp. 19-25 ◽  
Author(s):  
Eiichi Kawamoto ◽  
Takuo Sawada ◽  
Toru Sato ◽  
Kiyoshi Suzuki ◽  
Tsutomu Maruyama
Keyword(s):  
Immunosorbent Assay ◽  
Pasteurella Multocida ◽  
Rabbit Serum ◽  
Enzyme Linked Immunosorbent Assay ◽  
Post Inoculation ◽  
Serum Samples ◽  
Serological Tests ◽  
Indirect Haemagglutination ◽  
Precipitin Test ◽  
Gel Diffusion

Enzyme-linked immunosorbent assay (ELISA), gel-diffusion precipitin test (GDPT), and indirect haem agglutination test (IHAT) were evaluated for the detection of antibodies to Pasteurella multocida in both naturally and experimentally infected rabbits. A total of 285 rabbit serum samples from 7 rabbit colonies were tested by ELISA, GDPT, and IHAT, and nasal cultures were taken coincidentally to use as the standard in the serological tests. There was better correlation (98.0%) between the results of ELISA and positive nasal culture than between the GDPT (86.3%) or IHAT (23.5%) and positive nasal culture. In addition, ELISA and GDPT were positive in 26 (11.1%) and 21 (9.0%) of 234 serum samples from nasal culture negative rabbits, respectively. In experimentally infected rabbits, antibodies detected by the ELISA and GDPT began to rise one to 3 weeks post-inoculation. IHAT did not detect antibodies. These results are discussed in terms of value to serodiagnosis of rabbit pasteurellosis.

Autoantibodies to topoisomerase I (Scl-70): Analysis by gel diffusion, immunoblot, and enzyme-linked immunosorbent assay

1990 ◽  
Vol 57 (3) ◽  
pp. 399-410 ◽  
Author(s):  
Sabine Hildebrandt ◽  
Ethan S. Weiner ◽  
Jean-Luc Senecal ◽  
G.Stephen Noell ◽  
William C. Earnshaw ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Topoisomerase I ◽  
Enzyme Linked Immunosorbent Assay ◽  
Gel Diffusion
Sign in / Sign up

Export Citation Format

Share Document