Analysis of Hepatitis C Virus NS5A Region in Patients with Cirrhosis Using an Ultra-Deep Pyrosequencing Method

2017 ◽  
Vol 63 (09/2017) ◽  
Author(s):  
Fahriye Keskin ◽  
Sevgi Ciftci ◽  
Filiz Akyuz ◽  
Neslihan Abaci ◽  
Aris Cakiris ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Ns5a Region ◽  
Pyrosequencing Method

Subtype 2c of hepatitis C virus is highly prevalent in Italy and is heterogeneous in the NS5A region.

1997 ◽  
Vol 35 (1) ◽  
pp. 161-164 ◽  
Author(s):  
F Maggi ◽  
M L Vatteroni ◽  
C Fornai ◽  
A Morrica ◽  
M Giorgi ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Ns5a Region

scholarly journals Analysis of genotypes and amino acid residues 2209 to 2248 of the NS5A region of hepatitis C virus in relation to the response to interferon-? therapy

Hepatology ◽  
1997 ◽  
Vol 25 (3) ◽  
pp. 750-753 ◽  
Author(s):  
M Kurosaki ◽  
N Enomoto ◽  
T Murakami ◽  
I Sakuma ◽  
Y Asahina ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Amino Acid ◽  
Interferon Therapy ◽  
Amino Acid Residues ◽  
Ns5a Region

Association of interleukin 28B and mutations in the core and NS5A region of hepatitis C virus with response to peg-interferon and ribavirin therapy

2011 ◽  
Vol 31 (9) ◽  
pp. 1359-1365 ◽  
Author(s):  
Kazuhiko Hayashi ◽  
Yoshiaki Katano ◽  
Takashi Honda ◽  
Masatoshi Ishigami ◽  
Akihiro Itoh ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
The Core ◽  
Interleukin 28B ◽  
Ns5a Region

scholarly journals Direct visualization of hepatitis C virus-infected Huh7.5 cells with a high titre of infectious chimeric JFH1-EGFP reporter virus in three-dimensional Matrigel cell cultures

2014 ◽  
Vol 95 (2) ◽  
pp. 423-433 ◽  
Author(s):  
Shuanghu Liu ◽  
Ren Chen ◽  
Curt H. Hagedorn
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Cell Cultures ◽  
Three Dimensional ◽  
Egfp Expression ◽  
Reporter Virus ◽  
Egfp Gene ◽  
3D Cultures ◽  
Egfp Reporter ◽  
Ns5a Region

Identification of the hepatitis C virus (HCV) JFH1 isolate enabled the development of infectious HCV cell culture systems. However, the relatively low virus titres and instability of some chimeric JFH1 reporter viruses restricts some uses of this system. We describe a higher-titre JFH1-EGFP reporter virus where the NS5A V3 region was replaced with the EGFP gene and adapted by serial passage in Huh7.5 cells. Six adaptive mutants were identified: one each in E2, P7 and NS4B, plus three in the NS5A region. These adaptive mutants increased the reporter virus titres to 1×106 immunofluorescent focus-forming units ml−1, which is the highest titre of JFH1-EGFP reporter virus reported to our knowledge. This chimeric virus did not lose EGFP expression following 40 days of passage and it can be used to test the activity of HCV antivirals by measuring EGFP fluorescence in 96-well plates. Moreover, this reporter virus allows living infected Huh7.5 cells in Matrigel three-dimensional (3D) cultures to be visualized and produces infectious viral particles in these 3D cultures. The chimeric NS5A-EGFP infectious JFH1 reporter virus described should enable new studies of the HCV life cycle in 3D cell cultures and will be useful in identifying antivirals that interfere with HCV release or entry.

scholarly journals Evolution of hepatitis C virus NS5A region in breakthrough patients during pegylated interferon and ribavirin therapy

2010 ◽  
Vol 17 (3) ◽  
pp. 208-216 ◽  
Author(s):  
H. J. Yuan ◽  
M. Jain ◽  
K. K. Snow ◽  
M. Gale Jr ◽  
W. M. Lee ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Pegylated Interferon ◽  
Ns5a Region
Sign in / Sign up

Export Citation Format

Share Document