scholarly journals An İn vitro Study on Anticancer Activity of Noscapine

2021 ◽  
pp. 72-80
Author(s):  
Funda Karabağ Çoban ◽  
İbrahim Bulduk ◽  
İzzet İslam ◽  
Hande Aytuğ
Keyword(s):  
Breast Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Cancer Cell Lines ◽  
Papaver Somniferum ◽  
Breast Cancer Cell Lines ◽  
Ld50 Value ◽  
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Aims: Breast cancer is the most common type of cancer among women and ranks second among the causes of female death in the world. In order to find a solution to breast cancer, different studies are being conducted for the treatment and the effects of different drugs and substances on this disease are intensively investigated. Boric acid has been shown to control the proliferation of certain types of cancer cells.Noscapine is one of the ingredients in Papaver somniferum (opium). It was first isolated from Papaver somniferum (opium) in 1817. It is one of the most abundant opioids found in the opium plant (up to 10% of the total composition) after morphine. It is also known as Narcotine, Nectodon, Nospen, Anarcotine, and (archaic) Opiane and occurs in the (-)α isomer which has S, R stereochemistry (S stereochemistry at phthalide-carbon and R at isoquinoline-carbon). Noscapine is structurally and chemically different from other opium alkaloids such as morphine, codeine, thebaine, papaverine, and narceine. Materials and Methods: Based on this information, this study was conducted in vitro to optimize the pure form of noscapine (obtained from the poppy capsule) by applying different concentrations on MCF-7 breast cancer cell lines. HPLC technique which is one of the most widely analytical techniques has been used in this study. Determination of the LD50 value and cell proliferation by viability test was also performed to investigate the predicted effects of noscapine on MCF-7 breast cancer cell lines using VEGF (Vascular Endothelial Growth Factor) and PARP (Poly (ADP-Ribose) Polymerase) Analysis. Discussion: According to the results, it was observed in proliferation experiment that the vitality values decreased in direct proportion to the concentration and time at concentrations of 5 ppm, 10 ppm, 25 ppm, 50 ppm, 75 ppm, and 100 ppm. The LD50 value was determined as 50 ppm. There was no significant difference in VEGF values. It was also observed that the PARP level was lower than control group. Conclusion: As a result of the vitality test performed with the CCK-8 kit, it was determined that noscapine has an antiproliferative effect in various concentrations. The low PARP data in the noscapine groups suggests that the cell goes to apoptotic death.

scholarly journals In vitro investigation of cytotoxic and antioxidative activities of Ardisia crispa against breast cancer cell lines, MCF-7 and MDA-MB-231

2018 ◽  
Vol 18 (1) ◽  
Author(s):  
Muhammad Luqman Nordin ◽  
Arifah Abdul Kadir ◽  
Zainul Amiruddin Zakaria ◽  
Rasedee Abdullah ◽  
Muhammad Nazrul Hakim Abdullah
Keyword(s):  
Breast Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines ◽  
In Vitro Investigation ◽  
Ardisia Crispa ◽  
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scholarly journals In vitro Cytotoxicity Activity of Chrysin, Morin and Resveratrol Against MCF-7 Breast Cancer Cell Lines

2016 ◽  
Vol 13 (3) ◽  
pp. 1633-1637 ◽  
Author(s):  
Arlene Thomas ◽  
Niraja Ranadive ◽  
Harisha Nayak ◽  
Sneha Surendran ◽  
Madhavan Nampoothiri ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Cancer Cell Lines ◽  
In Vitro Cytotoxicity ◽  
Breast Cancer Cell Lines ◽  
Cytotoxicity Activity ◽  
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scholarly journals Computational, Pharmacological and Toxicological Approach of Repurposed Lamotrigine Schiff Base Derivatives for Reduction of Hormone-Positive Breast Tumor

2021 ◽  
Author(s):  
Saima Najm ◽  
Humaira Naureen ◽  
Fareeha Anwar ◽  
Muhammad Mubbashir Khan ◽  
Rabia Ali
Keyword(s):  
Breast Cancer ◽  
Schiff Base ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines ◽  
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Abstract Background and objectives: Breast cancer presents high morbidity among women with various treatment challenges. This study aims to evaluate the repurposed lamotrigine schiff base metal (LTG-SB-M) coordinates against in-vitro MCF-7 breast cancer cell lines and in-vivo N-methylnitrosourea (NMU)-persuaded toxicity of rats’ mammary gland. Method: In-silico computational analysis and in vitro cytotoxic studies on MCF-7 breast cancer cell lines was executed to build up the assumptions. In-vivo NMU-induced anticancer potential was assessed in forty Wistar rats; assigned into five groups of 8 rats each. Group I served as normal control and received normal saline, Group II received NMU (50 mg/kg), Group III received tamoxifen, whereas; Group IV and V received LTG-SB-M derivative (LAC3, LBC3) at dose of 100 mg/kg body weight, for 15 consecutive days. Intraperitoneal injection of NMU (single dose) was given at the age of 5, 9 and 13 weeks to the rats with the three week interval. For all experimental animals; biochemical markers were assessed. DNA strand breakage alongside the hormonal profile of estrogen and progesterone was also estimated. Results: All tested compounds present significant activity against MCF-7 cell lines in vitro and NMU-induced mammary tumor in vivo. The in vivo results of tested compounds present a significant decrease in weight of organ; with reinstated renal and hepatic enzymes. Histological analysis revealed strong countenance of proteins, estrogen, and progesterone in NMU-treated rats. Conclusion: These results suggest that LTG-SB-M complex can be used as better anticancer agent against breast cancer.

In vitro Cytotoxic Activity of Vatica diospyroides Symington Type LS Root Extract on Breast Cancer Cell Lines MCF-7 and MDA-MB-468

2013 ◽  
Vol 13 (2) ◽  
pp. 130-135 ◽  
Author(s):  
Theera Srisawat ◽  
Parinuch Chumkaew ◽  
Warapond Maichum ◽  
Yaowapa Sukpondma ◽  
Potchanapond Graidist ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines ◽  
Root Extract ◽  
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Evaluation of nonlinear optical differences between breast cancer cell lines SK-BR-3 and MCF-7; an in vitro study

2018 ◽  
Vol 23 ◽  
pp. 171-175 ◽  
Author(s):  
Alireza Ghader ◽  
Ali Abbasian Ardakani ◽  
Habib Ghaznavi ◽  
Ali Shakeri-Zadeh ◽  
Soraya Emamgholizadeh Minaei ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Nonlinear Optical ◽  
In Vitro Study ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines ◽  
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Design, Synthesis and Cytotoxicity of Novel Coumarin-1,2,3-triazole-1,2,4- Oxadiazole Hybrids as Potent Anti-breast Cancer Agents

2019 ◽  
Vol 16 (7) ◽  
pp. 818-824 ◽  
Author(s):  
Maryam Mohammadi-Khanaposhtani ◽  
Kiana Fahimi ◽  
Elahe Karimpour-Razkenari ◽  
Maliheh Safavi ◽  
Mohammad Mahdavi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Click Reaction ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines ◽  
Design Synthesis ◽  
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Background: This work reports design, synthesis, and in vitro cytotoxicity of novel coumarin-1,2,3-triazole-1,2,4-oxadiazole hybrids against three breast cancer cell lines MCF-7, MDA-MB-231, and T-47D. Methods: Synthetic procedure for the preparation of desired compounds was started from the reaction of coumarins or with propargyl bromide to give O-propargylated coumarins or 5. Then, click reaction between the later compounds and 3-aryl-5-(chloromethyl)-1,2,4-oxadiazoles afforded the desired products in good yields. Results: Among the synthesized compounds, 4-((1-((3-(4-chlorophenyl)-1,2,4-oxadiazol-5- yl)methyl)-1H-1,2,3-triazol-4-yl)methoxy)-2H-chromen-2-one (9a) showed the best cytotoxicity against breast cancer cell lines. Conclusion: Compound 9a depicted the most activity toward MDA-MB-231 and T-47D cells while compounds 8a and 8c were the most potent compounds against MCF-7.

Cytotoxic activity and anti-cancer potential of Ontario grown onion extracts against breast cancer cell lines

2018 ◽  
Vol 8 (3) ◽  
pp. 159 ◽  
Author(s):  
Meghan Fragis ◽  
Abdulmonem I. Murayyan ◽  
Suresh Neethirajan
Keyword(s):  
Breast Cancer ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines ◽  
Cytotoxic Activities ◽  
Cancer Line ◽  
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Background: Breast cancer is the most commonly diagnosed cancer and the second leading cause of cancer deaths among Canadian women. Cancer management through changes in lifestyle, such as increased intake of foods rich in dietary flavonoids, have been shown to decrease the risk associated with breast, liver, colorectal, and upper-digestive cancers in epidemiologic studies. Onions are high in flavonoid content and one of the most common vegetables. Additionally, onions are used in most Canadian cuisines.Methods: We investigated the effect of five prominent Ontario grown onion (Stanley, Ruby Ring, LaSalle, Fortress, and Safrane) extracts on two subtypes of breast cancer cell lines: a triple negative breast cancer line MDA-MB-231 and an ER+ breast cancer line MCF-7.Results: These onion extracts elicited strong anti-proliferative, anti-migratory, and cytotoxic activities on both the cancer cell lines. Flavonoids present in these onion extracts induced apoptosis, cell cycle arrest in the G2/M phase, and a reduction in mitochondrial membrane potential at dose-dependent concentrations. Onion extracts were more effective against MDA-MB-231 compared to the MCF-7 cell line. Conclusion: In this study, we investigated the extracts synthesized from Ontario-grown onion varieties in inducing anti-migratory, cytostatic, and cytotoxic activities in two sub-types of human breast cancer cell lines. Anti-tumor activity of these extracts depends upon the varietal and can be formulated into nutraceuticals and functional foods for the wellbeing of cancer patients. Overall, the results suggest that onion extracts are a good source of flavonoids with anti-cancerous properties.Keywords: onion extracts; flavonoids; anti-proliferative; breast cancer; cytotoxic activity

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