Characterization of Spinal Sensorimotor Network Using Transcutaneous Spinal Stimulation during Voluntary Movement Preparation and Performance

Transcutaneous electrical spinal stimulation (TSS) can be used to selectively activate motor pools based on their anatomical arrangements in the lumbosacral enlargement. These spatial patterns of spinal motor activation may have important clinical implications, especially when there is a need to target specific muscle groups. However, our understanding of the net effects and interplay between the motor pools projecting to agonist and antagonist muscles during the preparation and performance of voluntary movements is still limited. The present study was designed to systematically investigate and differentiate the multi-segmental convergence of supraspinal inputs on the lumbosacral neural network before and during the execution of voluntary leg movements in neurologically intact participants. During the experiments, participants (N = 13) performed isometric (1) knee flexion and (2) extension, as well as (3) plantarflexion and (4) dorsiflexion. TSS consisting of a pair pulse with 50 ms interstimulus interval was delivered over the T12-L1 vertebrae during the muscle contractions, as well as within 50 to 250 ms following the auditory or tactile stimuli, to characterize the temporal profiles of net spinal motor output during movement preparation. Facilitation of evoked motor potentials in the ipsilateral agonists and contralateral antagonists emerged as early as 50 ms following the cue and increased prior to movement onset. These results suggest that the descending drive modulates the activity of the inter-neuronal circuitry within spinal sensorimotor networks in specific, functionally relevant spatiotemporal patterns, which has a direct implication for the characterization of the state of those networks in individuals with neurological conditions.

EXPRESS: Visually Guided Movement with Increasing Time-on-Task: Differential Effects on Movement Preparation and Movement Execution

Top-down cognitive control seems to be sensitive to the detrimental effects of fatigue induced by time-on-task (ToT). The planning and preparation of the motor responses may be especially vulnerable to ToT. Yet, effects of ToT specific to the different phases of movements have received little attention. Therefore, in three experiments, we assessed the effect of ToT on a mouse-pointing task. In Experiment 1, there were 16 possible target positions with variable movement directions. In Experiment 2, the layout of the targets was simplified. In Experiment 3, using cuing conditions we examined whether the effects of ToT on movement preparation and execution were caused by an increased orientation deficit or decreased phasic alertness. In each experiment, initiation of movement (preparatory phase) became slower, movement execution became faster and overall response time remained constant with increasing ToT. There was, however, no significant within-person association between the preparatory and execution phases. In Experiments 1 and 2, we found a decreasing movement time/movement error ratio, suggesting a more impulsive execution of the pointing movement. In addition, ToT was also accompanied with imprecise movement execution as indicated by the increased errors, mainly in Experiment 2. The results of Experiment 3 indicated that ToT did not induce orientation and phasic alerting deficits but rather was accompanied by decreased tonic alertness.

Disrupted basal ganglia output during movement preparation in hemi-parkinsonian mice is consistent with behavioral deficits

Parkinsonian motor deficits are associated with elevated inhibitory output from the basal ganglia (BG). However, several features of Parkinson's disease (PD) have not been accounted for by this simple "classical rate model" framework, including the observation in PD patients that movements guided by external stimuli are less impaired than otherwise-identical movements generated based on internal goals. Is this difference due to divergent processing within the BG itself, or to the recruitment of extra-BG pathways by sensory processing? In addition, surprisingly little is known about precisely when, in the sequence from selecting to executing movements, BG output is altered by PD. Here, we address these questions by recording activity in the SNr, a key BG output nucleus, in hemiparkinsonian mice performing a well-controlled behavioral task requiring stimulus-guided and internally-specified directional movements. We found that hemiparkinsonian mice exhibited a bias ipsilateral to the side of dopaminergic cell loss that was stronger when movements were internally specified rather than stimulus guided, consistent with clinical observations in parkinsonian patients. We further found that changes in parkinsonian SNr activity during movement preparation were consistent with the ipsilateral behavioral bias, as well as its greater magnitude for internally-specified movements. While these findings are inconsistent with some aspects of the classical rate model, they are accounted for by a related "directional rate model" positing that SNr output phasically over-inhibits motor output in a direction-specific manner. These results suggest that parkinsonian changes in BG output underlying movement preparation contribute to the greater deficit in internally-specified than stimulus-guided movements.

Response bias reveals the role of interhemispheric inhibitory networks in movement preparation and execution

Human movement is influenced by various cognitive processes, such as bias, that dynamically shape competing movement representations. However, the neurophysiological mechanisms underlying the effects of bias on movement selection across the lifespan remains poorly understood.Healthy young (n = 21) and older (n = 20) adults completed a choice reaction time task necessitating left- or right-hand responses to imperative stimuli (IS). Response bias was manipulated via a cue that informed participants a particular response was 70% likely (i.e., the IS was either congruent, or incongruent, with the cue); biasing was either fixed for blocks of trials (block-wise bias) or varied from trial-to-trial (trial-wise bias). As well as assessing the behavioural manifestations of bias, we used transcranial magnetic stimulation to determine changes in corticospinal excitability (CSE) and short- and long-interval interhemispheric inhibition (SIHI, LIHI) during movement preparation and execution. Participants responded more quickly, and accurately, in congruent compared to incongruent trials. CSE decreases occurred in both hands following the cue, consistent with the ‘inhibition for impulse control’ hypothesis of preparatory inhibition. In contrast, IHI modulations occurred in a hand-specific manner. Greater SIHI was observed during movement preparation in the hand biased away from, compared to the hand biased towards, the cue; furthermore, greater SIHI was observed during movement execution in the hand biased towards the cue when it was not required to respond (i.e., incongruent trial) compared to when it was required to respond (congruent trial). Additionally, during the movement preparation period, the LIHI ratio of the hand biased towards, compared to the hand biased away from, the cue was greatest when the cue varied trial-by-trial. Overall, the IHI results provide support for the ‘inhibition for competition resolution’ hypothesis, with hand specific modulation of inhibition during movement preparation and execution.