Sustainable, cost-efficient manufacturing of therapeutic peptides using chemo-enzymatic peptide synthesis (CEPS)

Exemplified by antidiabetic exenatide CEPS provides a highly efficient, easily scalable biocatalytic route to manufacture therapeutic peptides.

Design of a substrate-tailored peptiligase variant for the efficient synthesis of thymosin-α1

A substrate-tailored peptide ligase for the chemo-enzymatic peptide synthesis (CEPS) of thymosin-α1.

Thiol–ene photoimmobilization of chymotrypsin on polysiloxane gels for enzymatic peptide synthesis

Here we report a strategy to photoimmobilize chymotrypsin onto polysiloxane gels via thiol-ene click chemistry. The polysiloxane-immobilized chymotrypsin shows enhanced catalytic activity, thermal stability and recyclability.